Retinal safety of the irradiation delivered to light-adjustable intraocular lenses evaluated in a rabbit model.

Purpose: To evaluate the safety to the retina of a light-delivery device used to irradiate a light-adjustable intraocular lens (IOL) after implantation in a rabbit model.

Setting: John A. Moran Eye Center, University of Utah, Salt Lake City, Utah, USA.

Uropathogenic Escherichia coli CFT073 is adapted to acetatogenic growth but does not require acetate during murine urinary tract infection.

In vivo accumulation of D-serine by Escherichia coli CFT073 leads to elevated expression of PAP fimbriae and hemolysin by an unknown mechanism. Loss of D-serine catabolism by CFT073 leads to a competitive advantage during murine urinary tract infection (UTI), but loss of both D- and L-serine catabolism results in attenuation. Serine is the first amino acid to be consumed in closed tryptone broth cultures and precedes the production of acetyl phosphate, a high-energy molecule involved in intracellular signaling, and the eventual secretion of acetate.

Prevention of endophthalmitis by collagen shields presoaked in fourth-generation fluoroquinolones versus by topical prophylaxis.

Purpose: To compare the prophylaxis of collagen shields presoaked in antibiotics versus antibiotic drops after bacterial anterior chamber challenge.

Setting: John A. Moran Eye Center, University of Utah, Salt Lake City, Utah, USA.

Roles of serine accumulation and catabolism in the colonization of the murine urinary tract by Escherichia coli CFT073.

A D-serine deaminase (DsdA) mutant of uropathogenic Escherichia coli strain CFT073 has a hypercolonization phenotype in a murine model of urinary tract infection (UTI) due to increased virulence gene expression by an unknown mechanism (B. J. Haugen et al.

Corneal endothelial safety with the irradiation system for light-adjustable intraocular lenses.

Purpose: To assess the safety to the corneal endothelium of the irradiation delivered through a system developed for noninvasive postoperative power adjustments of the Light Adjustable Lens (LAL) (Calhoun Vision).

Setting: John A. Moran Eye Center, University of Utah, Salt Lake City, Utah, USA.

In vivo gene expression analysis identifies genes required for enhanced colonization of the mouse urinary tract by uropathogenic Escherichia coli strain CFT073 dsdA.

Deletional inactivation of the gene encoding d-serine deaminase, dsdA, in uropathogenic Escherichia coli strain CFT073 results in a hypermotile strain with a hypercolonization phenotype in the bladder and kidneys of mice in a model of urinary tract infection (UTI). The in vivo gene expression profiles of CFT073 and CFT073 dsdA were compared by isolating RNA directly from the urine of mice challenged with each strain individually. Hybridization of cDNAs derived from these samples to CFT073-specific microarrays allowed identification of genes that were up- or down-regulated in the dsdA deletion strain during UTI.

New photochromic foldable intraocular lens: preliminary study of feasibility and biocompatibility.

Purpose: To evaluate a new hydrophobic acrylic intraocular lens (IOL) with photochromic properties in vitro and in vivo in a rabbit model.

Setting: John A. Moran Eye Center, University of Utah, Salt Lake City, Utah, USA.

A light adjustable lens with injectable optics.

The light adjustable lens (LAL) is an innovative intraocular lens optic composed of partially polymerized macromers with an appropriately bonded photosensitizer. The injectable technology and multifocality associated with the LAL can produce precise refractive correction and, it is hoped, the type of accommodative range that is taken for granted during youth. Combining these technologies with a lens material that behaves like the crystalline lens of 25-year-old could precisely return near and distance vision to older adults.

A light adjustable lens with injectable optics.

Purpose Of Review: Technology exists today that could permit refractive precision unheard of at the present time plus the correction of higher-order aberrations and restoration of accommodation in presbyopic adults.

Recent Findings: With the light adjustable lens, after surgery, the shape of the lens can be customized to treat spherical, cylindrical and other higher-order aberrations. The posterior surface of the lens can be adjusted to create both refractive multifocality and diffractive bifocality.

Coordinate expression of fimbriae in uropathogenic Escherichia coli.

Uropathogenic Escherichia coli is the most common etiological agent of urinary tract infections. Bacteria can often express multiple adhesins during infection in order to favor attachment to specific niches within the urinary tract. We have recently demonstrated that type 1 fimbria, a phase-variable virulence factor involved in adherence, was the most highly expressed adhesin during urinary tract infection.

Transcriptome of uropathogenic Escherichia coli during urinary tract infection.

A uropathogenic Escherichia coli strain CFT073-specific DNA microarray that includes each open reading frame was used to analyze the transcriptome of CFT073 bacteria isolated directly from the urine of infected CBA/J mice. The in vivo expression profiles were compared to that of E. coli CFT073 grown statically to exponential phase in rich medium, revealing the strategies this pathogen uses in vivo for colonization, growth, and survival in the urinary tract environment.

Uropathogenic Escherichia coli use d-serine deaminase to modulate infection of the murine urinary tract.

Although once thought to be unique to bacteria, d-amino acids are also produced by mammals. For example, d-serine is excreted in human urine at concentrations ranging from 3.0 to 40 micro g ml-1.