Publications by authors named "Brian Glenn St Hilaire"
Article Synopsis
- * The type of genome structure present in an organism is linked to the presence of condensin II, a protein complex involved in chromosome organization.
- * Depleting condensin II in humans leads to a genome architecture similar to simpler organisms, suggesting a conserved mechanism from our common ancestor that impacts how genomes are structured during cell division.
Article Synopsis
- Eukaryotic genomes are structured into loops formed by cohesin complexes, which can be stabilized by CTCF or removed by WAPL.
- Some loops remain stable for hours despite cohesin typically having a short residence time due to WAPL's actions.
- In G1-phase, acetylated cohesin can bind chromatin for extended periods, and both CTCF and the acetyltransferase ESCO1 play key roles in maintaining these long-lived loops and enhancing chromatin organization.
Cohesin extrusion is thought to play a central role in establishing the architecture of mammalian genomes. However, extrusion has not been visualized in vivo, and thus, its functional impact and energetics are unknown. Using ultra-deep Hi-C, we show that loop domains form by a process that requires cohesin ATPases.
View Article and Find Full Text PDFThe human genome folds to create thousands of intervals, called "contact domains," that exhibit enhanced contact frequency within themselves. "Loop domains" form because of tethering between two loci-almost always bound by CTCF and cohesin-lying on the same chromosome. "Compartment domains" form when genomic intervals with similar histone marks co-segregate.
View Article and Find Full Text PDF50 years ago, Vincent Allfrey and colleagues discovered that lymphocyte activation triggers massive acetylation of chromatin. However, the molecular mechanisms driving epigenetic accessibility are still unknown. We here show that stimulated lymphocytes decondense chromatin by three differentially regulated steps.
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