Publications by authors named "Brian C Lim"
Axon initial segment (AIS) cell surface proteins mediate key biological processes in neurons including action potential initiation and axo-axonic synapse formation. However, few AIS cell surface proteins have been identified. Here, we use antibody-directed proximity biotinylation to define the cell surface proteins in close proximity to the AIS cell adhesion molecule Neurofascin.
View Article and Find Full Text PDFAxon initial segment (AIS) cell surface proteins mediate key biological processes in neurons including action potential initiation and axo-axonic synapse formation. However, few AIS cell surface proteins have been identified. Here, we used antibody-directed proximity biotinylation to define the cell surface proteins in close proximity to the AIS cell adhesion molecule Neurofascin.
View Article and Find Full Text PDFRapid and efficient saltatory action potential conduction depends on the myelin sheath and clustered Na channels at nodes of Ranvier. A new study convincingly shows that the periaxonal space is a necessary conductive component to accurately model myelinated axon physiology and saltatory conduction.
View Article and Find Full Text PDFArticle Synopsis
- AISs are crucial for generating action potentials and maintaining protein, lipid, and organelle distribution in neurons, but their stabilization and mechanisms of neuronal polarity are not fully understood.
- Researchers used advanced techniques like proximity biotinylation and mass spectrometry to identify proteins associated with the AIS, revealing many previously unidentified biotinylated proteins.
- The study highlights the role of these proteins in interacting with known AIS components and how their absence can disrupt AIS structure and function, offering new insights into AIS organization and stability.