Live Cell Analysis of Shear Stress on Pseudomonas aeruginosa Using an Automated Higher-Throughput Microfluidic System.

A higher-throughput microfluidic in vitro bioreactor coupled with fluorescence microscopy has been used to study bacterial biofilm growth and morphology, including Pseudomonas aeruginosa (P. aeruginosa). Here, we will describe how the system can be used to study the growth kinetics and the morphological properties such as the surface roughness and textural entropy of P.

The Quantitative Assessment of (PA)14 Biofilm Surface Coverage on Slippery Liquid Infused Polymer Surfaces (SLIPS).

Slippery, porous polymeric antimicrobial surfaces for biofilm attachment inhibition of the clinical strain (PA14) have been prepared. Porous BMA-EDMA, characterized for its hydrophobic properties, was infused with a slippery liquid creating a hydrophobic liquid interface and characterized by water contact angle and SEM. A low shear force bioreactor was used to prepare biofilms on these antimicrobial surfaces.

Dominant mutations in the late 40S biogenesis factor Ltv1 affect cytoplasmic maturation of the small ribosomal subunit in Saccharomyces cerevisiae.

In eukaryotes, 40S and 60S ribosomal subunits are assembled in the nucleus from rRNAs and ribosomal proteins, exported as premature complexes, and processed in final maturation steps in the cytoplasm. Ltv1 is a conserved 40S ribosome biogenesis factor that interacts with pre-40S complexes in vivo and is proposed to function in yeast in nuclear export. Cells lacking LTV1 grow slowly and are significantly impaired in mature 40S subunit production.