Publications by authors named "Brett D Abraham"

Article Synopsis
  • - Mesenchymal stromal cells (MSCs) are promising for cell therapy in various diseases due to their ability to secrete beneficial factors and modulate the immune system, but producing enough cells for clinical use is challenging.
  • - Suspension bioreactors combined with microcarriers allow for scalable MSC production, but traditional microcarriers often use animal proteins, raising regulatory concerns.
  • - A novel recombinant fusion protein was developed to attach growth factors to cellulose microcarriers without animal components, enhancing MSC growth and production in bioreactors while reducing costs and improving regulatory compliance.
View Article and Find Full Text PDF

Background: While pluripotent stem cell (PSC) therapies move toward clinical and commercial applications at a rapid rate, manufacturing reproducibility and robustness are notable bottlenecks in regulatory approval. Therapeutic applications of PSCs require large cell quantities to be generated under highly robust, well-defined, and economically viable conditions. Small-scale and short-term process optimization, however, is often performed in a linear fashion that does not account for time needed to verify the bioprocess protocols and analysis methods used.

View Article and Find Full Text PDF

Water ecosystem contamination from industrial pollutants is an emerging threat to both humans and native species, making it a point of global concern. In this work, fully biobased aerogels (FBAs) were developed by using low-cost cellulose filament (CF), chitosan (CS), citric acid (CA), and a simple and scalable approach, for water remediation applications. The FBAs displayed superior mechanical properties (up to ∼65 kPa m kg specific Young's modulus and ∼111 kJ/m energy absorption) due to CA acting as a covalent crosslinker in addition to the natural hydrogen bonding and electrostatic interactions between CF and CS.

View Article and Find Full Text PDF

A scalable method for the assembly of oriented bacterial cellulose (BC) films is presented based on using wrinkled thin silicone substrates of meter-square size as templates during biotechnological syntheses of BC. Control samples, including flat templated and template-free bacterial cellulose, along with the oriented BC, are morphologically characterized using scanning electron microscopy (SEM). Multiple functional properties including wettability, birefringence, mechanical strength, crystallinity, water retention, thermal stability, .

View Article and Find Full Text PDF