Molecular analysis and chromosomal assignment of the canine CALC-I/alpha-CGRP gene.

We have isolated a recombinant phage harboring the canine CALC-I/alpha-CGRP gene. The gene spans a region of approx. 5.

Genomic structure of the 5' end of the porcine ryanodine receptor 3 gene (RYR3).

Ryanodine receptor 3 is a calcium channel located in the membrane of the endoplasmic reticulum. We isolated eight overlapping PAC clones from the porcine ryanodine receptor 3 gene (RYR3) and determined the DNA sequences of the first and second exon together with 5.8 kb of 5' flanking region and 10.

Genomic structures and sequences of two closely linked genes (AMT, TCTA) on dog chromosome 20q15.1-->q15.2.

Analysis of genomic sequence from canine chromosome 20q15.1-->q15.2 revealed the presence of two closely linked genes.

Cloning, structural organization, and chromosomal assignment of the porcine c-fos proto-oncogene, FOS.

The complete porcine c-fos proto-oncogene (FOS) with flanking regions was cloned and sequenced. FOS consists of four exons at amino acids 1-47, 48-131, 132-167, and 168-380 and includes all the typical motifs of the fos proto-oncogene. The promoter contains consensus sequences for CRE, SRE, CaRE, and the E-Box, as well as an AP-1 site.

Structural analysis and transcript processing of the bovine proteolipid protein (PLP) gene.

In this study we present the complete genomic structure of the bovine PLP gene and its assignment to the long arm of the X-chromosome (BTXq2.1). We determined a total of 18,767 bp of the bovine PLP gene and compared it to the human heterolog.

Genomic organization of the dog dystroglycan gene DAG1 locus on chromosome 20q15.1-q15.2.

Dystroglycan is a laminin binding protein, which provides a structural link between the subsarcolemmal cytoskeleton and the extracellular matrix. It is also involved in the organization of basement membranes. So far the genomic organization of the dystroglycan gene DAG1 has not been completely investigated.

Analysis of blood clotting factor activities in canine Legg-Calvé-Perthes' disease.

Legg-Calve-Perthes' (LCP) disease is a noninflammatory aseptic necrosis of the femoral head and neck in small-breed dogs. The etiology of the disease is not known, but ischemia resulting from vascular compression or occlusion has been proposed. A latent ischemic phase during development of the femoral epiphysis seems to be responsible for the onset of the typical clinical features of LCP disease.

Isolation and characterization of the porcine c-myc proto-oncogene and chromosomal assignment to SSC 4p13.

The proto-oncogene c-myc codes for a nuclear phosphoprotein, a transcription factor composed of the typical basic/helix-loop-helix/leucine zipper domains. Its expression is coupled to a multitude of physiological processes and regulated by a variety of hormones, growth factors, cytokines, lymphokines and the nutritional status, development and differentiation. Its key roles have been characterized, e.

Molecular analysis of the porcine proteolipid protein (PLP) gene.

The proteolipid protein (PLP) gene codes for the most abundant protein in the central nervous system (CNS) myelin of higher vertebrates. Its function in the myelin sheath is not clear however, a series of point mutations have been shown to have devastating effects on the myelin. The structure of the PLP genes is highly conserved, comprising seven exons that code for an open reading frame of 277 amino acids.

Conserved nucleotide differences and subfamily structure of porcine short interspersed elements.

Interspersed elements are ubiquitous in the genomes of higher eukaryotes and account for over a third of the genomic DNA (Smit 1996). In swine the short interspersed elements, SINEs or PREs (porcine repetitive elements), have been found in a number of introns and 3' untranslated regions of different genes. However, compared to human Alu repeats the number of available PRE DNA sequences is still limited.

Construction and characterization of a porcine P1-derived artificial chromosome (PAC) library covering 3.2 genome equivalents and cytogenetical assignment of six type I and type II loci.

A porcine P1-derived artificial chromosome (PAC) library of a male German Landrace pig was constructed in pCYPAC2. In total 90,240 clones were generated and individually transferred into microtiter plates. An average insert size of 119.

Zinc finger proteins: watchdogs in muscle development.

The specificity of highly differentiated tissues is largely achieved through the action of cell- and stage-restricted transcription factors. The basic events in skeletal muscle development are triggered by a unique family of myogenic basic helix-loop-helix proteins - MyoD, Myf-5, myogenin and MRF-4. Binding sites for these factors are found in the promoter regions of many genes whose expression is restricted to muscle cells, but the tight regulation of gene expression is dependent on the interaction of different factors.

Molecular cloning and chromosomal assignment of the porcine 54 and 56 kDa vacuolar H(+)-ATPase subunit gene (V-ATPase).

Vacuolar proton-translocating ATPases (V-ATPase) are multisubunit enzyme complexes located in the membranes of eukaryotic cells regulating cytoplasmic pH. So far, nothing is known about the genomic organization and chromosomal location of the various subunit genes in higher eukaryotes. Here we describe the isolation and analysis of a cDNA coding for the 54- and 56-kDa porcine V-ATPase subunit alpha and beta isoforms.

Molecular characterization and chromosomal assignment of the canine protein C gene.

Protein C is a precursor to a serine protease present in the plasma that plays an important physiological role in the regulation of blood coagulation. Mutations in the human protein C gene have been linked to some cases of Morbus Perthes disease, a thrombophilic condition that results in aseptic necrosis of the femur head and neck. We have cloned the canine protein C gene to investigate whether Morbus Perthes disease in dogs is also caused by mutations within this gene.