Development of macaque secondary follicles exposed to neutral red prior to 3-dimensional culture.

Purpose: Neutral red (NR) may assist identification of preantral follicles in pieces of cortical tissue prior to cryopreservation in cancer patients requesting fertility preservation. This study is the first to analyze this effect by follicle growth rate after long-term culture in primates.

Methods: Ovarian cortex was obtained from adult rhesus macaques, was cut into fragments, and was incubated with NR.