[HIV virus detection in ejaculates collected at different times in seropositive patients].

Ejaculates from 22 seropositive males were collected at various times 1 to 11 ejaculates for each in one year period of time. After fractionation, no virus is detected in mobile and living spermatozoa. Moreover, HIV is not always found in all ejaculates from the same patient.

[Study of HIV localization in sperm].

The aim of the study was to investigate the presence and the localization of HIV in human ejaculate and its different components. Sixty-three ejaculates from 19 HIV-positive patients have been studied. By using cellular as well as molecular biology methods, we never detected HIV in living and mobile spermatozoa although we sometime found the virus in seminal liquid and in nuclear fractions.