Peritoneal dialysis is an established method of treatment of chronic renal failure. In that paper morphological and functional changes of peritoneum due to the process of long-term dialysis are presented. Morphological changes are observed in mesothelial cells, intercellular junctions, interstitial tissue and blood vessels.
View Article and Find Full Text PDFJ Physiol Pharmacol
September 1996
Because of the evidence that peritoneal macrophages are activated during peritoneal dialysis, we hypothesised that the injury of the peritoneum is, at least in part, dependent on the intraperitoneal generation of free radicals. The aim of the study was to evaluate the effect of vitamin E on the peroxidation and permeability of the peritoneum during chronic peritoneal dialysis in rats. Supplementation of the intraperitoneally infused saline with vitamin E decreased the peroxidation of peritoneum estimated as the malondialdehyde (MDA) level in rats' omentum.
View Article and Find Full Text PDFObjective: To assess effects of the inflammatory cytokines (IL-1-beta, TNF-alpha, TGF-beta 1) and dialysate effluent on synthesis of hyaluronic acid by human peritoneal mesothelial cells (HMC) in in vitro culture.
Methods: Dialysate effluent was collected after the overnight dwell of Dianeal 1.5% from patients during CAPD training.
Effect of peritoneal dialysis fluids on glutathione (GSH/GSSG) level in human peritoneal mesothelial cells was tested in in vitro experiments. To mimic in vivo conditions, cells were initially exposed to dialysis fluids (Dianeal 1.36%, Dianeal 2.
View Article and Find Full Text PDFThe interest in structure and function of peritoneum increased when it turned out that it may be used as biologic dialysis membrane. The structure of peritoneum is of significant value in the course of peritoneal dialysis. The role of the mesothelium, the interstitium, blood and lymphatic capillaries, major constituents of peritoneum, have been deeply studied.
View Article and Find Full Text PDFThe prevalence of sensitization to common inhalant allergens was assessed among schoolchildren using skin prick tests with standard allergen extracts. The results were analysed in relation to asthma and allergy suggestive symptoms. A positive reaction to at least one allergen was observed in 13.
View Article and Find Full Text PDFHydrogen peroxide can be indirectly detected in mesothelial cells by measuring the rate of inhibition of the intracellular catalase activity by 3-amino-1, 2,3-triazole (AT). AT binds and inhibits catalase only in the presence of hydrogen peroxide, and the effect is proportional to the amount of hydrogen peroxide. The effect of AT on catalase activity is prevented in the presence of ethanol.
View Article and Find Full Text PDFPlanimetric studies of peritoneal surface area were performed in 10 humans, 12 rabbits, and 15 rats. It was found that the total peritoneal surface area (TPSA) correlated in humans with body surface area (BSA) (r = 0.98, p < 0.
View Article and Find Full Text PDFL-2-Oxothiazolidine-4-carboxylate and N-acetylcysteine as substrates for intracellular glutathione in human peritoneal mesothelial cells were tested. Both substances at concentrations of 0.01 mM and higher augmented the level of glutathione in mesothelial cells.
View Article and Find Full Text PDFRecent studies have indicated that atopic sensitisation is uncommon while respiratory symptoms are common among schoolchildren in Eastern Europe. Risk factors for respiratory symptoms and atopic sensitisation were evaluated in a cross sectional study involving 2594 schoolchildren (10-12 years) from Sweden (n = 665), Poland (n = 410), and Estonia (n = 1519). The measurements included parental questionnaires and skin prick tests with eight standardised allergens.
View Article and Find Full Text PDFThe authors tested in vitro the effect of glucose-based and amino acid-based dialysate effluent on the function of human peritoneal mesothelial cells. After 9 days of exposure to the tested effluents with medium (1:1 v/v) or to a medium supplemented with 10% fetal calf serum (FCS) (control), several functional properties of the cells were studied. The synthesis of DNA measured by incorporation of 3H-methyl-thymidine was higher in mesothelial-cell monolayers exposed to the dialysates than in the controls.
View Article and Find Full Text PDFRecent studies have demonstrated that intraperitoneal instillation of dialysis solutions and drug additives may adversely affect the function of peritoneal cell populations. Therefore the aim of the present investigation was to examine the short-term effects of antineoplastic agents on human peritoneal mesothelial cells (HPMC). We have assessed the integrity of HPMC membrane and mechanisms of intracellular potassium transport.
View Article and Find Full Text PDFPneumonol Alergol Pol
September 1995
Many reports describe an increase in the incidence of allergies in recent years. Thus the epidemiological studies are necessary for efficacious prophylaxis. The aim of our study was to estimate the prevalence of allergic diseases in schoolchildren.
View Article and Find Full Text PDFObjective: To evaluate the changes in biocompatibility of peritoneal dialysis solutions during intraperitoneal dwell.
Design: We studied the effect of the drained dialysates at time 0 and after 30, 60, 120, 240, and 360 minutes of intraperitoneal dwell on the growth of peritoneal mesothelial cells and fibroblasts and the synthesis of proteins by these cells. On one day the patients were dialyzed with glucose-based Dianeal and on alternate days with an amino acid-containing solution based on Travasol.
Clin Exp Allergy
September 1994
Allergic sensitization and symptoms from the airways in relation to air pollution were compared in 10-12-year-old school children (n = 1113) from urban Konin in central Poland and both urban and rural parts of Sundsvall in northern Sweden. The measurements included parental questionnaires, skin-prick tests and serial peak flow measurements during 2 weeks with simultaneous monitoring of outdoor air pollutants. The skin-prick test technique was validated by IgE antibody determinations.
View Article and Find Full Text PDFThere is a growing need for more biocompatible peritoneal dialysis fluid formulations. Using a method of in vitro cell culture we have examined the effect of osmotic agents, antiseptics and drug additives on various functions of human peritoneal mesothelial cells (HPMC). We have demonstrated that glucose and amino acids reduce the HPMC proliferation rate in a dose-dependent manner.
View Article and Find Full Text PDFObjective: To study how the presence of osmotic solutes in medium affects growth of the peritoneal mesothelial cells and fibroblasts and how osmotic solutes influence the production of factors regulating growth of these cells.
Design: The proliferation of mesothelial cells and fibroblasts was evaluated by measuring the incorporation of 3H-thymidine into the cells. Cells were exposed to osmotic solutes; the concentration of the latter in the medium was continuously lowered over the time of the experiment to simulate changes of their concentration in the dialysate.