Effect of rumen development and pre-exposure to chemicals on the activity of the mixed function oxidase system in goats.

1. The activity of the mixed function oxidase (MFO) system of the lung and liver of goats were measured in three age groups. The induction of the MFO system by sodium phenobarbital (PB) and the inhibition of piperonyl butoxide (BT) in each age group were also determined.

The effect of dietary protein quantity on the activity of UDP-glucuronyltransferase and its physiological significance in drug metabolism.

Low dietary protein has been shown to induce the activity of rat hepatic UDP-glucuronyltransferase (UDPGTase) as measured in vitro. The assay of UDPGTase in vitro is hampered by the need to solubilize the microsomal membrane, without destroying the physiological significance of the measurements. The present work was to determine the effect of dietary protein on the activity of UDPGTase and on the activity of UDP-glucose dehydrogenase.

Correlation among the changes in the levels of thyroid hormones, thyrotropin and thyrotropin-releasing hormone during the development of Xenopus laevis.

The influence of thyroid hormones on neurogenesis suggested that these hormones might affect the maturation and development of neural centers which ultimately regulate thyroid function. Changes in the levels of the thyroid hormones, thyrotropin and the thyrotropin-releasing hormone in 'brain' and 'thyroid' fractions were, therefore, correlated at several developmental stages. All hormones showed increases from their initial detection through metamorphosis.

Glycogen accumulation in alveolar type II cells in 3-methylindole--induced pulmonary edema in goats.

The present study shows that intravenous infusion of 3-methylindole (3MI) induced acute pulmonary edema in goats. Edematous changes were seen in the alveoli and the interalveolar interstitium. At 72 hours after treatment, an accumulation of glycogen that had a pathognomonic appearance of alpha particles was observed in the alveolar Type II cells.

Tissue and subcellular distribution and excretion of 3-[14C]methylindole in rabbits after intratracheal infusion.

The tissue and subcellular distribution and urinary excretion pattern of radioactivity in rabbits after intratracheal administration of a 14C-labeled pulmonary toxin, 3-methylindole (3MI), was studied. Approximately 40% of the administered radioactivity appeared in the urine within 1 h but none of the radioactivity in the urine was 3MI. The lung had a higher concentration of radioactivity than the liver.

Weight gain of rats fed medium-chain triglycerides is less than rats fed long-chain triglycerides.

Weight gain was measured in rats fed test diets with two types of fat: corn oil and medium-chain triglycerides (MCT). These diets were selected because fatty acids from corn oil enter the circulation as chylomicrons through the lacteals. Fatty acids from medium-chain triglycerides reach the liver directly through the portal circulation.

Role of mixed-function oxidase in 3-methylindole-induced acute pulmonary edema in goats.

The relationship between the pulmonary toxicity of 3- methylindole (3MI, skatole) and the mixed-function oxidase (MFO) system was investigated. Nine goats assigned to three groups were given a jugular infusion of [14C]3MI (0.02 to 0.

3-methylindole-induced pulmonary injury in goats.

Ruminal administration of 3-methylindole in goats severe pulmonary edema and respiratory distress. Electron microscopic studies of lungs reveal extensive degeneration and necrosis of alveolar membranous pneumocytes and bronchiolar epithelium. The necrosis of the pneumocytes is followed by proliferation of granular pneumocytes, which repopulate the alveolar basal lamina scaffold.

Replacement of condylar process.

Three cases of replacement of the condylar process have been presented with their courses and the type of prosthesis that was chosen. Each case has a unique cause with different needs for the use of a prosthesis.

An EPR study of structural perturbations induced by methylindole in the protein and lipid regions of erythrocyte membranes.

3-Methylindole has been shown in previous work to cause pulmonary edema and emphysema in cattle and goats. In this paper, evidence is presented to show that 3-methylindole induces structural perturbations in bovine erythrocyte membranes. The structural perturbations which were induced as a function of 3-methylindole concentration in the membranes were measured by EPR using the attachment of maleimide spin label to the sulfhydryl groups of membrane proteins and by intercalation of methyl-5- doxylstearate, methyl-12-doxylstearate, and methyl-16-doxylstearate into the lipid region.

The effects of 3-methylindole on hemolysis, transport of Na+, and ATPase activities of bovine erythrocytes.

Biochemical effects of 3MI on cellular membranes were investigated. This study was conducted to examine the effects of 3MI on the hemolysis of erythrocytes, the transport of 22Na+ in resealed erythrocyte ghosts, and on the ATPase activities of erythrocyte membranes. The percent of hemolysis as a function of 3MI incubation time was sigmoidal.