Indirect evidence of glomerular/tubular mixed-type postexercise proteinuria in healthy humans.

Hypothetical mechanisms have been postulated to explain the presence of proteins in urine after severe exercise. Recently, it has been shown that several amino acids inhibit tubular protein reabsorption. Seven healthy men, hyperhydrated, were studied during a 2-min bicycle exercise at supramaximal load.

[Comparative study of the diagnostic contribution of C-terminal and medio-regional determination of parathyroid hormone in man].

In this study, we compared serum parathyrin radioimmunoassay values obtained with three commercially available kits in a series of normal subjects, patients on dialysis, patients with primary hyperparathyroidism and with hypercalcemia due to malignancy. The calcium of these subjects was simultaneously evaluated. Two of these three kits measure two different C-terminal portions of the molecule and the third the mid region of PTH.

Relative contribution of various expressions of cAMP excretion to other indices of parathyroid function, as tested by discriminant multivariate linear regression analysis.

We evaluated the relative contribution to the diagnosis of hyperparathyroid disease from current laboratory indices of parathyroid function--plasma calcium (I), phosphate (II), carboxy-terminal (III) and predominantly amino-terminal (IV) radioimmunoassays of parathyrin, the urinary excretion ratios of cyclic adenosine monophosphate (cAMP) to creatinine (V) or to glomerular filtrate (VI), and the ratio of the nephrogenous fraction of cAMP to glomerular filtrate (VII)--in 224 subjects: 40 with surgically proven hyperparathyroid disease, the others normoparathyroid. The decreasing order of sensitivity was I greater than VI greater than VII greater than V greater than III greater than IV greater than II; all these indices differed significantly between normoparathyroid and hyperparathyroid patients. The decreasing order of specificity was VII, III greater than I greater than IV greater than V, II greater than VI.

Magnesium administration reverses the hypocalcaemia secondary to hypomagnesaemia despite low circulating levels of 25-hydroxyvitamin D and 1,25-dihydroxy vitamin D.

The effect of parenteral administration of magnesium was studied in five patients with hypomagnesaemic hypocalcaemia. The initial metabolic state was characterized by a normal level of serum immunoreactive parathyroid hormone (iPTH), and by low or undetectable serum 25-hydroxyvitamin D (25OHD) and 1,25-dihydroxyvitamin D (1,25 (OH)2D). A parathyroid response was elicited by the acute intravenous injection of magnesium chloride.

Prolactin and growth hormone response to levodopa in affective illness.

Prolactin (PRL) and growth hormone (GH) response to L-Dopa have been studied in 51 affectively ill women (26 unipolar and 25 bipolar) before and after amitriptyline treatment and in 14 normal female controls. There was no difference in GH response to L-dopa in all groups studied except for bipolar postmenopausal women, who showed a blunted GH response to L-Dopa compared to bipolar premenopausal women. After amitriptyline treatment, no difference in GH response was found in all groups studied.

Measure of blood ionized calcium versus total calcium in normal man, in renal insufficiency and in hypercalcemia of various origins.

Ca++ and Ca tot were measured and compared at 2 years interval (S1 and S2) in 45 normal volunteers: 26 females and 19 males age 20/65 years. For Ca++ the mean difference between S1 and S2 was small (0.009 mmol/l) but significant (p less than 0.

Thyrotrophin response to thyrotrophin-releasing hormone in unipolar and bipolar affective illness.

The plasma levels of thyrotrophin (TSH) and the response of this hormone to 200 microgram of thyrotrophin-releasing hormone (TRH) were studied in 27 unipolar and 24 bipolar depressive patients before and after amitriptyline treatment as well as in 42 normal controls. There was no significant difference in basal TSH levels between any of the groups studied according to diagnosis, menopausal status and antidepressant treatment. Before treatment, the TSH response to TRH was significantly lower in the unipolar and bipolar depressive patients than in normal controls.

Thyroid status in elderly sick patients.

Thyroid investigations were performed on 55 euthyroid patients hospitalized for chronic disease or recovering from acute illness. Three age groups were considered. Abnormalities were found only in patients over 75 years: 1) in the TRH test the maximum increase in serum TSH (delta TSHmax) was less marked and frequently delayed to 60 min, whereas the increase in T3 in 120 min (delta T3) was maintained; basal TSH was normal; 2) in 15 out of 28 cases, the pattern of circulating thyronines was abnormal showing an increase in reverse T3 a normal or decreased T3, and a rise in free T4 index beyond the normal range in 3 cases.

Prolactin secretion in women with unipolar and bipolar depression.

Plasma prolactin levels and prolactin response to thyrotropin releasing hormone (TRH) were studied in 27 unipolar and 24 bipolar depressive female patients before and after tricyclic antidepressant treatment, as well as in 38 normal controls matched for age, sex, and menopausal status. Before antidepressant treatment, basal prolactin levels were significantly lower in both premenopausal and postmenopausal bipolar patients but only in postmenopausal unipolar patients when compared to controls. The prolactin response to TRH was significantly blunted in both unipolar and bipolar postmenopausal subjects but remained normal in all premenopausal (unipolar and bipolar) patients.

Growth hormone after TRH in women with depressive illness.

Plasma growth hormone (GH) release after TRH injection was investigated in 27 unipolar and 23 bipolar depressive women, and in 39 normal female controls matched for age, sex and menopausal status. Peaks of GH secretion after TRH were observed in some depressive patients as well as in some normal controls, but there were no significant differences in GH response after TRH administration between patients and controls. The implications of these findings are discussed in reference to such factors as non-specific stress responses and abnormal neuroendocrine circadian rhythms in affectively ill patients.

[Endocrinological abnormalities in the neurotic states and in depression (author's transl)].

Endocrinological investigation of depressed patients shows a wide variety of abnormalities, most of them reflecting disturbed hypothalamo-hypophyseal control mechanisms. Reported results however vary widely among investigators reflecting the heterogeneity of the clinical cases and the difficulties of quantifying depression. The authors report data on 1(0) TSH, PRL and HGH secretion after TRH in a group of 19 endogenous depressed patients; 2(0) insulin secretions after a glucose load in endogenous (10 cases) and neurotic (13 cases) depression; 3(0) LH and FSH secretion after LHRH in 10 neurotic depressed men.

Synovial fluid beta 2 microglobulin and hydroxyproline fractions in rheumatoid arthritis and nonautoimmune arthropathies.

Fourteen patients with classical and definite seropositive rheumatoid arthritis (RA), 5 patients with microcrystalline arthritis, and 7 patients with osteoarthrosis were studied with respect to markers of newly synthesised collagen (synovial NDOH pro levels); markers of connective tissue resorption (synovial DOH pro and NBH levels); markers of lymphoid tissue activity (synovial and plasma beta 2m levels). Higher amounts of NDOH pro in RA synovial fluid are compatible with the hypothesis of a local connective tissue production as suggested by Uitto et al. on basis of a higher protocollagen proline hydroxylase activity in RA synovial tissue.

Plasma beta 2 microglobulin as a means of predicting gentamicin serum concentrations.

A novel and accurate method for predicting gentamicin peak serum concentration is described. The method considers body weight and renal function as determined by the concentration of beta 2 microglobulin in plasma. In 32 subjects whose renal function ranged from normal to severely impaired, the peak serum concentration of gentamicin was more closely correlated with beta 2 microglobulin (r = 0.

Assessment of a plasma ADH radioimmunoassay in experimental and physiologic or pathologic conditions.

A radioimmunoassay of ADH has been applied to the study of plasma ADH levels in various conditions. The validity of the assay has been evaluated by the usual quality control parameters of RIA and by the measure of plasma levels in 12 upright water deprived normal volunteers (mean 9.5 pg/ml, SEM +/- 1.