Changes in the Microbiome During Chronic Rhinosinusitis.

Chronic rhinosinusitis (CRS) is a common inflammatory disease of the paranasal sinuses with a yet unknown etiology. As studies continue to elucidate the disease's heterogeneity inflammatory profile and presentation, there is a growing interest in the influence of the nasal microbiome on disease pathogenesis and chronicity. The sinus microbiota appear dominated by the and genera; known upper airway pathogens, such as , are present in the upper airways of healthy individuals, though at relatively lower abundances than in CRS patients.

Caring for older parents in Norway - How does it affect labor market participation and absence from work?

As the population ages, younger generations will increasingly be called upon to provide informal care to their aging parents. To prepare for this development, it is essential to understand how employees combine the dual responsibilities of work and caring for aging parents. By analyzing data collected in Norway in 2022 from a nationally representative sample of 6049 respondents, aged 35 to 67, we investigated how caring for older parents affects labor market participation and work absence.

After Hospital: Should Older Care-Needing Patients Be Transferred to Their Homes or to an Intermediate Care Institution?

Background: In most European countries, communities need to provide health and social care services to an increasing number of severely ill patients discharged from hospitals. We investigated whether nurses in hospitals and in the communities' health and social care services experienced that the administration in the municipalities allocated older patients the right type of services after hospital discharge.

Methods: We used data from Norway, with a qualitative pilot study and quantitative analysis (structural equation modeling) of surveys involving 2431 nurses on inpatient wards in acute hospitals and 4312 nurses working in nursing homes or home nursing.

Nurses' experiences of transitions of older patients from hospitals to community care. A nation-wide survey in Norway'.

Moving older patients from hospitals to community services is a critical phase of integrated care. Yet there has been little large-scale research on the quality of these transitions. We investigated how Norwegian nurses working in community care services (N = 4312) and at in-patient wards at hospitals (N = 2421) experienced the quality of transitions of older patients from hospitals to community care.

Supporting the old but neglecting the young? The two faces of ageism.

Ageism is the most prevalent form of prejudice and is experienced by both older and younger people. Little is known about whether these experiences are interdependent or have common origins. We analyze data from 8,117 older (aged 70 and over) and 11,647 younger respondents (15-29 years) in representative samples from 29 countries in the European Social Survey.

Should I stay or should I go? Nurses' wishes to leave nursing homes and home nursing.

Aims: This study investigates the prevalence of nurses' wishes to leave work in elderly care services and aims to explain differences between younger and older nurses.

Background: Health-and-care services, and specifically elderly care services, experience problems recruiting and retaining nurses.

Method: A nationwide survey among nurses in Norway with 4,945 nurses aged 20-73 (mean age = 41.

Perceived age discrimination across age in Europe: From an ageing society to a society for all ages.

Ageism is recognized as a significant obstacle to older people's well-being, but age discrimination against younger people has attracted less attention. We investigate levels of perceived age discrimination across early to late adulthood, using data from the European Social Survey (ESS), collected in 29 countries (N = 56,272). We test for approximate measurement invariance across countries.

Caring too much? Lack of public services to older people reduces attendance at work among their children.

The need to provide care for older people can put a strain on their adult children, potentially interfering with their work attendance. We tested the hypothesis that public care for older people (nursing homes or home care services) would moderate the association between having an older parent in need of care and reduced work attendance among the adult children. The analysis used data from a survey of Norwegian employees aged 45-65 ( = 529).

What do older people think that others think of them, and does it matter? The role of meta-perceptions and social norms in the prediction of perceived age discrimination.

Psychological theories of aging highlight the importance of social context. However, very little research has distinguished empirically between older people's perception of how others in their social context perceive them (personal meta-perceptions) and the shared perceptions in society (societal meta-perceptions). Drawing on theories of intergroup relations and stereotyping and using a multilevel perspective, this article examines how well older people's perceptions of age discrimination (PAD) are predicted by (a) older people's personal meta-perceptions, (b) societal meta-perceptions, and (c) social norms of intolerance toward age prejudice.

Shaping the Development of Prejudice: Latent Growth Modeling of the Influence of Social Dominance Orientation on Outgroup Affect in Youth.

Social dominance orientation (SDO) has been theorized as a stable, early-emerging trait influencing outgroup evaluations, a view supported by evidence from cross-sectional and two-wave longitudinal research. Yet, the limitations of identifying causal paths with cross-sectional and two-wave designs are increasingly being acknowledged. This article presents the first use of multi-wave data to test the over-time relationship between SDO and outgroup affect among young people.

One of few or one of many: Social identification and psychological well-being among minority youth.

Feeling belongingness with small social groups such as the family or a group of friends predicts psychological well-being. Acculturation research has argued for similar effects of belongingness with large social groups. In particular, a strong ethnic identity is assumed to improve psychological well-being among members of minority groups, but this conclusion has been drawn based on cross-sectional data.

'Being old and ill' across different countries: social status, age identification and older people's subjective health.

Objective: It has been suggested that the extent to which older adults identify with 'old-age' is associated with greater subjective ill-health. Based on social identity theory, we hypothesise that the societal social status of older people should moderate this relationship, such that the effect of age-identification on subjective health should be stronger in countries in which older people have lower social status.

Design And Main Outcome Measures: Subjective health, age identification and the perceived status of people over 70 were assessed in a subsample of older respondents (N = 6185) of the 2008/2009 European Social Survey.

Subjective social status of older people across countries: the role of modernization and employment.

Objectives: To test and expand upon modernization theory's account of subjective social status (SSS) of older people in society. It was hypothesized that perceptions of older people's social status should be higher in more modernized countries and that the proportion of older people in employment should moderate the relationship between modernization and SSS of older people.

Methods: Data were from the "Experiences and Expressions of Ageism" module in the fourth round of the European Social Survey.

Perceived Age Discrimination as a Mediator of the Association Between Income Inequality and Older People's Self-Rated Health in the European Region.

Objectives: The relative income hypothesis predicts poorer health in societies with greater income inequality. This article examines whether the psychosocial factors of perceived age discrimination and (lack of) social capital may help explain the adverse effect of inequality on older people's health.

Methods: Self-rated health, perceived age discrimination, and social capital were assessed in the 2008/9 European Social Survey (European Social Survey Round 4 Data, 2008).

Revisiting the paradox of well-being: the importance of national context.

Objectives: Despite age-related changes or declines in circumstances, health or income, many older people are able to maintain subjective well-being (SWB) in later life. This is known as the paradox of well-being. To date, much research has focused on either individual- (e.

Why men (and women) do and don't rebel: effects of system justification on willingness to protest.

Three studies examined the hypothesis that system justification is negatively associated with collective protest against ingroup disadvantage. Effects of uncertainty salience, ingroup identification, and disruptive versus nondisruptive protest were also investigated. In Study 1, college students who were exposed to an uncertainty salience manipulation and who scored higher on system justification were less likely to protest against the governmental bailout of Wall Street.

Communication: Antimicrobial Activity of SMAP28 with a Targeting Domain for Porphyromonas gingivalis.

Antibiotic therapy is often used with mechanical therapy to treat periodontal disease. However, complications associated with antibiotic use can occur. A 'bacteria-specific' targeted approach would eliminate some of these complications and kill specific periodontopathogens without harming the commensal bacteria.

What will the others think? In-group norms as a mediator of the effects of intergroup contact.

The influence of social norms in the context of intergroup relations has long been recognized by social psychologists, yet research on intergroup contact and social norms have usually remained disconnected. We explored the influence of direct and indirect friendship on attitudes towards ethnic minorities in Norway, and in particular the role of in-group norms about the social approval of intergroup contact as a mechanism that distinguishes direct from indirect contact. Using a sample of school students from 89 classrooms (N=823), we tested this hypothesis with both one level and multi-level structural equation modelling (ML-SEM), where the amount of contact of other classroom members was considered as a form of indirect contact.

Differences in human macrophage receptor usage, lysosomal fusion kinetics and survival between logarithmic and metacyclic Leishmania infantum chagasi promastigotes.

The obligate intracellular protozoan, Leishmania infantum chagasi (Lic) undergoes receptor-mediated phagocytosis by macrophages followed by a transient delay in phagolysosome maturation. We found differences in the pathway through which virulent Lic metacyclic promastigotes or avirulent logarithmic promastigotes are phagocytosed by human monocyte-derived macrophages (MDMs). Both logarithmic and metacyclic promastigotes entered MDMs through a compartment lined by the third complement receptor (CR3).

Identification of proteins released by mammalian cells that mediate DNA internalization through proteoglycan-dependent macropinocytosis.

Naked DNA plasmid represents the simplest vehicle for gene therapy and DNA-based vaccination purposes; however, the molecular mechanisms of DNA uptake in mammalian cells are poorly understood. Here, we show that naked DNA uptake occurs via proteoglycan-dependent macropinocytosis, thus challenging the concept of a specific DNA-internalizing receptor. Cells genetically deficient in proteoglycans, which constitute a major source of cell-surface polyanions, exhibited substantially decreased uptake of likewise polyanionic DNA.

Regression analysis and modelling of data acquisition for SELDI-TOF mass spectrometry.

Motivation: Pre-processing of SELDI-TOF mass spectrometry data is currently performed on a largel y ad hoc basis. This makes comparison of results from independent analyses troublesome and does not provide a framework for distinguishing different sources of variation in data.

Results: In this article, we consider the task of pooling a large number of single-shot spectra, a task commonly performed automatically by the instrument software.

In-source decay causes artifacts in SELDI-TOF MS spectra.

SELDI-TOF MS is a mass spectrometric technique which has been extensively used for biomarker discovery. In this study, we show that in-source decay is an important source for the generation of additional spectral peaks with this technique, both for pure proteins and proteins in serum samples. Thus, SELDI-TOF MS could be used to gain sequence information from proteins, but the results also question the uncritical use of SELDI-TOF MS as a general method for the detection of biomarkers.

Transforming growth factor-beta 1 specifically induce proteins involved in the myofibroblast contractile apparatus.

Transforming growth factor-beta(1) (TGF-beta(1)) induces alpha-smooth muscle actin (alpha-SMA) and collagen synthesis in fibroblast both in vivo and in vitro and plays a significant role in tissue repair and the development of fibrosis. During these processes the fibroblasts differentiate into activated fibroblasts (so called myofibroblasts), characterized by increased alpha-SMA expression. Because TGF-beta(1) is considered the main inducer of the myofibroblast phenotype and cytoskeletal changes accompany this differentiation, the main objective of this investigation was to study how TGF-beta(1) alters protein expression of cytoskeletal-associated proteins.

Restricted access chromatographic sample preparation of low mass proteins expressed in human fibroblast cells for proteomics analysis.

Two-dimensional electrophoresis and modern image analysis systems have made it possible to study protein expression and regulation of proteins in biological systems. Proteins in the molecular mass region of 20-120 kDa are well investigated and described. However, proteins with masses below 20 kDa are the least investigated as they are rarely seen on 2D-PAGE due to fast migrations in the electric field and lack of staining efficiency.

Purification and identification of the violaxanthin deepoxidase as a 43 kDa protein.

Violaxanthin deepoxidase (VDE) has been purified from spinach (Spinacia oleracea) leaves. The purification included differential sonication of thylakoid membranes, differential (NH4)2SO4 fractionation, gel filtration chromatography and finally either hydrophobic interaction chromatography or anion exchange chromatography. A total purification of more than 5000-fold compared to the original thylakoids enabled the identification of a 43 kDa protein as the VDE, in contrast to earlier reported molecular weight of 54-60 kDa.