Small RNA CjNC110 regulates the activated methyl cycle to enable optimal chicken colonization by .

Post-transcriptional gene regulation by non-coding small RNAs (sRNAs) is critical for colonization and survival of enteric pathogens, including the zoonotic pathogen . In this study, we utilized IA3902 (a representative isolate of the sheep abortion clone) and W7 (a highly motile variant of NCTC 11168, a human gastroenteritis strain) to further investigate regulation by sRNA CjNC110. Both motility and autoagglutination ability were confirmed to be phenotypes of conserved regulation by CjNC110.

Direct interaction of small non-coding RNAs CjNC140 and CjNC110 optimizes expression of key pathogenic phenotypes of .

Small non-coding RNAs (sRNAs) are important players in modulating gene expression in bacterial pathogens, but their functions are largely undetermined in , an important cause of foodborne gastroenteritis in humans. In this study, we elucidated the functions of sRNA CjNC140 and its interaction with CjNC110, a previously characterized sRNA involved in the regulation of several virulence phenotypes of . Inactivation of CjNC140 increased motility, autoagglutination, L-methionine concentration, autoinducer-2 production, hydrogen peroxide resistance, and early chicken colonization, indicating a primarily inhibitory role of CjNC140 for these phenotypes.

Enrofloxacin Alters Fecal Microbiota and Resistome Irrespective of Its Dose in Calves.

Enrofloxacin is a fluoroquinolone drug used to prevent and control bovine respiratory disease (BRD) complex in multiple or single doses, ranging from 7.5 to 12.5 mg/kg body weight.

Role of in Methionine Metabolism and Optimal Chicken Colonization in Campylobacter jejuni.

is a zoonotic pathogen and is one of the leading causes of human gastroenteritis worldwide. IA3902 (representative of the sheep abortion clone) is genetically similar to W7 (representative of strain type NCTC 11168); however, there are significant differences in the ability of mutants of these strains to colonize chickens. LuxS is essential for the activated methyl cycle and generates homocysteine for conversion to l-methionine.

Small Noncoding RNA CjNC110 Influences Motility, Autoagglutination, AI-2 Localization, Hydrogen Peroxide Sensitivity, and Chicken Colonization in Campylobacter jejuni.

Small noncoding RNAs (ncRNAs) are involved in many important physiological functions in pathogenic microorganisms. Previous studies have identified the presence of noncoding RNAs in the major zoonotic pathogen ; however, few have been functionally characterized to date. CjNC110 is a conserved ncRNA in , located downstream of the gene, which is responsible for the production of the quorum sensing molecule autoinducer-2 (AI-2).

High Prevalence of Fluoroquinolone-Resistant Bacteria in Sheep and Increased Counts in the Bile and Gallbladders of Sheep Medicated with Tetracycline in Feed.

is a major foodborne pathogen in humans and a significant cause of abortion in sheep. Although ruminants are increasingly recognized as important reservoirs for species, limited information is available about the molecular epidemiology and antimicrobial resistance (AMR) profiles of sheep Here, we describe a two-trial study that examined profiles in sheep and determined whether in-feed tetracycline (TET) influenced the distribution and AMR profiles of Each trial involved 80 commercial sheep naturally infected with : 40 of these sheep were medicated with tetracycline in feed, while the other 40 received feed without antibiotics. Fecal and bile samples were collected for the isolation of The bacterial isolates were analyzed for antimicrobial susceptibility and genotypes.