Flotation-Based T Cell Isolation, Activation, and Expansion from Human Peripheral Blood Mononuclear Cell Samples Using Microbubbles.

The process of isolating T cells from peripheral blood mononuclear cells (PBMCs) to establish ex vivo cultures is crucial for research, clinical testing, and cell-based therapies. In this study, a simple, novel protocol to isolate, activate, and expand T cells from PBMCs ex vivo is presented. This study utilizes functionalized buoyancy-activated cell sorting (BACS) technology to isolate and activate T cells.

Nickel foil microcantilevers for magnetic manipulation and localized heating.

Cellular manipulation has been investigated by a number of techniques. In this manuscript nickel foil microcantilevers were used for magnetophoresis and manipulation of microparticles and magnetically labeled HeLa cells. The cantilevers were also used for localized heating in liquid, reaching biologically relevant temperatures.

Self-assembled magnetic bead biosensor for measuring bacterial growth and antimicrobial susceptibility testing.

Bacterial antibiotic resistance is one of the major concerns of modern healthcare worldwide, and the development of rapid, growth-based, antimicrobial susceptibility tests is key for addressing it. The cover image shows a self-assembled asynchronous magnetic bead rotation (AMBR) biosensor developed for rapid detection of bacterial growth. Using the biosensors, the minimum inhibitory concentration of a clinical E.

Asynchronous magnetic bead rotation microviscometer for rapid, sensitive, and label-free studies of bacterial growth and drug sensitivity.

The long turnaround time in antimicrobial susceptibility testing (AST) endangers patients and encourages the administration of wide spectrum antibiotics, thus resulting in alarming increases of multidrug resistant pathogens. A method for faster detection of bacterial proliferation presents one avenue toward addressing this global concern. We report on a label-free asynchronous magnetic bead rotation (AMBR) based viscometry method that rapidly detects bacterial growth and determines drug sensitivity by measuring changes in the suspension's viscosity.

Nanoparticle induced cell magneto-rotation: monitoring morphology, stress and drug sensitivity of a suspended single cancer cell.

Single cell analysis has allowed critical discoveries in drug testing, immunobiology and stem cell research. In addition, a change from two to three dimensional growth conditions radically affects cell behavior. This already resulted in new observations on gene expression and communication networks and in better predictions of cell responses to their environment.

Asynchronous magnetic bead rotation (AMBR) biosensor in microfluidic droplets for rapid bacterial growth and susceptibility measurements.

Inappropriate antibiotic use is a major factor contributing to the emergence and spread of antimicrobial resistance. The long turnaround time (over 24 hours) required for clinical antimicrobial susceptibility testing (AST) often results in patients being prescribed empiric therapies, which may be inadequate, inappropriate, or overly broad-spectrum. A reduction in the AST time may enable more appropriate therapies to be prescribed earlier.

Experimental system for one-dimensional rotational brownian motion.

We present here an experimental, strictly one-dimensional rotational system, made by using single magnetic Janus particles in a static magnetic field. These particles were half-coated with a thin metallic film, and by turning on a properly oriented external static magnetic field, we monitor the rotational brownian motion of single particles, in solution, around the desired axis. Bright-field microscopy imaging provides information on the particle orientation as a function of time.

Label-acquired magnetorotation for biosensing: An asynchronous rotation assay.

This paper presents a novel application of magnetic particles for biosensing, called label-acquired magnetorotation (LAM). This method is based on a combination of the traditional sandwich assay format with the asynchronous magnetic bead rotation (AMBR) method. In label-acquired magnetorotation, an analyte facilitates the binding of a magnetic label bead to a nonmagnetic solid phase sphere, forming a sandwich complex.

High frequency asynchronous magnetic bead rotation for improved biosensors.

Biosensors with increasingly high sensitivity are crucial for probing small scale properties. The asynchronous magnetic bead rotation (AMBR) sensor is an emerging sensor platform, based on magnetically actuated rotation. Here the frequency dependence of the AMBR sensor's sensitivity is investigated.

Monitoring the growth and drug susceptibility of individual bacteria using asynchronous magnetic bead rotation sensors.

Continuous growth of individual bacteria has been previously studied by direct observation using optical imaging. However, optical microscopy studies are inherently diffraction limited and limited in the number of individual cells that can be continuously monitored. Here we report on the use of the asynchronous magnetic bead rotation (AMBR) sensor, which is not diffraction limited.

Sudden breakdown in linear response of a rotationally driven magnetic microparticle and application to physical and chemical microsensing.

In this work, sensing magnetic microparticles were used to probe both the local pH and the viscosity-dependent nonlinear rotational behavior of the particles. The latter resulted from a critical transition marking a driven particle's crossover from phase-locking to phase-slipping with an externally rotating magnetic field, i.e.