TFC-1326 Compound Reduces Clinical Signs of Skin Aging. Evidence From In Vitro Human Adipose and Skin Models and Pilot Clinical Trial.

Background: Anti-freeze Glycoproteins (AFGPs) were described to preserve biological materials and protect them from different stresses.

Aims: The effects of a synthetic anti-freeze glycoprotein-based compound, TFC-1326, on human skin quality and its biological actions were studied.

Methods: The effects of various concentrations of TFC-1326 on the biology of human preadipocytes, differentiated in the proinflammatory microenvironment, and on human fibroblasts grown in coculture with human mature adipocytes or monocultured in stress conditions were investigated in, in vitro studies.

Anti-Inflammatory and Antioxidant Effects of Diosmetin-3--β-d-Glucuronide, the Main Metabolite of Diosmin: Evidence from Ex Vivo Human Skin Models.

Diosmin is used to relieve chronic venous disease (CVD) symptoms. This study aimed to investigate the anti-inflammatory and antioxidant effects of diosmetin-3--β-d-glucuronide, the major metabolite of diosmin, using human skin explants. The explants were exposed to substance P (inflammation model) or UVB irradiation (oxidative model) and to five diosmetin-3--β-d-glucuronide concentrations.

A Harungana madagascariensis extract with retinol-like properties: Gene upregulations and protein expressions in human fibroblasts and skin explants.

Objective: Because they limit, even reverse, age-induced skin alterations, retinoids became a staple in cosmetology. However, their use can result in undesired secondary effects and there is a demand for natural sources of compounds with retinoid-like effects. A preliminary screening identified a Harungana madagascariensis plant extract (HME) as possibly inducing genes stimulated by retinol.

Tasmannia lanceolata leaf extract alleviates stretch mark appearance in a randomized, placebo-controlled clinical trial in women and stimulates extracellular matrix synthesis in ex vivo human skin explants.

Background: The leaves of Tasmannia lanceolata mainly contain polygodial that is known to exhibit a range of biological functions including anti-inflammatory effects.

Aims: These studies aimed to assess the effects of Tasmannia lanceolata extract (TLE) on skin and more particularly on stretch marks in women.

Patients/methods: A double-blind, randomized, placebo-controlled clinical study was carried out on 29 women, aged from 25 to 60 years, to investigate the effects of TLE on stabilized stretch marks.

Purple tulip extract improves signs of skin aging through dermal structural modulation as shown by genomic, protein expression and skin appearance of volunteers studied.

Background: Purple tulip extract is a rich source of flavonoids which are powerful antioxidants and can hence be considered as an ideal candidate for use in skin care products.

Aims: We aimed to evaluate the effects of purple tulip extract on skin quality and to determine its molecular modes of interaction.

Methods: A pangenomic study on human skin fibroblasts was carried out to analyze multiple changes in gene expression.

Polar lipids from wheat extract oil improve skin damages induced by aging: Evidence from a randomized, placebo-controlled clinical trial in women and an ex vivo study on human skin explant.

Background: Polar lipids from wheat (Triticum vulgare/aestivum) extract oil (WEO) are known to improve skin hydration.

Aims: These studies aimed to assess WEO benefits on the skin appearance of middle-aged women.

Methods: A double-blind, randomized, placebo-controlled clinical study was carried out on 64 healthy women, aged from 45 to 60 years, to investigate antiaging effects and benefits for the skin.

Anti-inflammatory and antiradical effects of a 2% diosmin cream in a human skin organ culture as model.

Background: Diosmin, a naturally occurring flavonoid, is considered as a vascular-protective agent and is used orally to treat chronic venous insufficiency. It exhibits anti-inflammatory and free radical scavenging properties, but, like many other flavonoids, it is poorly absorbed in the small intestine.

Objective: Our aim was to investigate the skin protective effects of a diosmin-based cream, using skin organ culture as model.

Split-face histological and biochemical evaluation of tightening efficacy using temperature- and impedance-controlled continuous non-invasive radiofrequency energy.

Background: Bipolar radiofrequency (RF) is capable of heating dermal collagen fibers and inducing skin tightening by collagen remodeling.

Objective: To substantiate safety and improvement of skin laxity following skin heating with a novel temperature- and impedance-controlled non-invasive radiofrequency (RF) device by histological and biochemical evaluations.

Methods: A split-face study was performed on 4 subjects who underwent 8 weekly RF sessions on one side of their face, leaving the other side an untreated control and then underwent facelift procedure.

Ex-vivo study of hybrid energy technology using a human skin model.

Background: More aging adults and the social acceptance of aesthetic treatments have increased the demand for minimally invasive aesthetic treatments. Skin resurfacing is very effective at improving aging symptoms, including wrinkles and skin imperfections. Following the negative effects of full skin resurfacing, in addition to a very long downtime, fractional lasers and fractional radiofrequency (RF) technologies were introduced, since gaining popularity.

Anti-inflammatory and draining effect of the Deep Oscillation® device tested clinically and on a model of human skin maintained in survival condition.

The objective of this study was to evaluate the anti-inflammatory, toning and draining effect of the Deep Oscillation® device. The Deep Oscillation® device uses the forces of pulsed electrostatic attraction and friction to provoke oscillations that act on the epidermis, dermis, and sub-cutaneous layers of tissue. An ex-vivo study was first completed by using a model of skin maintained in survival condition.

Ex vivo study of the home-use TriPollar RF device using an experimental human skin model.

Background: A wide variety of professional radio frequency (RF) aesthetic treatments for anti-aging are available aiming at skin tightening. A new home-use RF device for facial treatments has recently been developed based on TriPollar technology.

Objective: To evaluate the mechanism of the new home-use device, in the process of collagen remodeling, using an ex vivo skin model.

Clinical and histopathological study of the TriPollar home-use device for body treatments.

Professional non invasive treatments for body contouring based on radiofrequency (RF) became popular in aesthetic clinics due to proven efficacy and safety. A new home-use RF device for body treatments has been developed based on TriPollar technology. Our objective was to evaluate the TriPollar home-use device for circumference reduction, cellulite improvement and skin tightening using objective and subjective methods.

Ex vivo human skin evaluation of localized fat reduction and anti-aging effect by TriPollar radio frequency treatments.

Background: A wide variety of radio frequency (RF) treatments for localized fat and cellulite reduction as well as anti-aging are available nowadays, but only a few have shown the biological mechanism responsible for the clinical results.

Objective: To determine the biological mechanism of the TriPollar RF device for localized fat and cellulite reduction as well as the collagen remodeling effect.

Methods: Human skin samples were collected from abdominoplasty surgery and facial lifts, in order to evaluate the lipolytic and anti-aging effects of the apollo device powered by TriPollar RF technology using an ex vivo human skin model.

Healing effect of a spray containing Rhealba oat colloidal extract in an in vitro reconstitution model of skin.

By using a reconstituted skin model, we aimed to evaluate the efficacy of a spray containing Rhealba oat extract on the rapidity of healing and the extension of the newly formed epithelium. A dermal equivalent was first made in a petri dish by combining skin fibroblasts with collagen type I. Then a punch biopsy as a source of epidermal cells was implanted on this dermal equivalent, where a multilayered epidermis developed.

Identification of clustered cells in human hair follicle responsible for MMP-9 gelatinolytic activity: consequences for the regulation of hair growth.

Background: The control of human hair follicle growth and differentiation is dependent upon several well-identified factors, including androgens, cytokines, and growth factors. In humans, alopecia androgenetica is a common aging process thought to be regulated through complex genetic imbalances, which also involve several of these crucial identified factors (and probably others not yet characterized), alone or in combination. Among these factors, epidermal growth factor (EGF), as well as pro-inflammatory cytokines, play a pivotal role, as evidenced by their direct inhibitory effects on hair growth both in vitro and in vivo.

Alteration of cytokeratin expression in oral lichen planus.

The purpose of this investigation is to examine the possible biochemical and topographic cytokeratin alterations in lichen planus of oral mucosa. Biopsy samples of clinically normal buccal mucosa (n = 5), normal gingiva (n = 5), lichen planus from buccal mucosa (n = 5), and lichen planus from gingiva (n = 5) were obtained from patients of both sexes. Cytokeratin expression was determined by means of immunohistochemical labeling with use of a battery of monoclonal antibodies against cytokeratins and filaggrin and two-dimensional gel electrophoresis.

[Topical tretinoin in the treatment of lichen planus and leukoplakia of the mouth mucosa. A clinical evaluation].

A randomized study was conducted to evaluate the effect of tretinoin and patient tolerance to treatment with topical applications in series of 20 cases of smoking-related or traumatic oral keratoses leukoplakia and of 20 cases of lichen planus. In each group, patients applied the topical ointment containing tretinoin (10 patients) or placebo (10 patients) twice daily. Clinical outcome was evaluated on the basis of the surface area of the lesion, measured monthly during treatment, as compared with the area observed at treatment onset.

[Topical tretinoin in the treatment of lichen planus and leukoplakia of the oral mucosa. A biochemical evaluation of the keratinization].

In earlier work, we demonstrated that 0.1 p. 100 topical tretinoin is clinically effective and well tolerated compared with placebo for the treatment of oral leukoplakia and oral keratosic or erythematous lichen planus.

Expression of HLA class II antigens on skin fibroblasts in scleroderma.

Skin biopsies were taken from 11 patients with morphoea, nine with acrosclerosis and 10 with diffuse systemic sclerosis and processed for immunohistochemical studies using a panel of monoclonal antibodies including antibodies to MHC class II antigens. A significantly higher percentage of HLA-DR positive dermal cells were observed in the reticular dermis in biopsies from patients with morphoea (44.1 +/- 16.

Morphometric analysis of dermal collagen fibers in normal human skin as a function of age.

A quantitative study of dermal collagen as a function of age was carried out by computerized digital image analysis. Fast Green-Syrius Red stained sections were obtained of skin biopsies taken from the upper inner arm of 33 healthy women and 38 healthy men. The Leitz texture Analysis System (Leitz-TAS) and mathematical morphology (Serra, 1982) were used for the evaluation of the data.

Changes in the elastic tissue of the non-sun-exposed skin of cigarette smokers.

Biopsies were taken from the upper and inner arm of 10 60-year-old male cigarette smokers and compared with 10 age-matched controls who were non-smokers. The mean relative area, number and thickness of the elastic fibres were significantly increased in the cigarette smokers compared to the controls. These results were confirmed using antibodies to elastin or the microfibrillar component of elastic tissue.

Immunohistochemical study of oral lesions of lichen planus: diagnostic and pathophysiologic aspects.

The immunophenotype of lymphoid cells in the epithelium and lamina propria of the oral mucosa were examined in patients with lichen planus, nondysplastic leukoplakia, leukoplakia with lichen planus, and other unrelated lesions. In all groups T lymphocytes were predominant; however, the T4/T8 lymphocyte ratio was higher with lichen planus than with other groups. This may be of diagnostic value in the histologic evaluation of oral lesions not typical of lichen planus.

Elastic fibers in normal human skin. Variations with age: a morphometric analysis.

A quantitative study of dermal elastic fibers was carried out by computerized digital image analysis. Sections of skin biopsies taken from the upper inner arm of 33 healthy women and 38 healthy men were stained by a selective procedure for elastic fibers. The Leitz texture analysis system (Leitz-Tas) and mathematical morphology were used for the evaluation of the data.

Skin thickness changes in normal aging skin.

The age-dependent decrease of skin thickness was studied with a morphometric procedure on upper inner arm skin biopsies. Epidermal thickness decreased somewhat faster in men (7.2% of the original value/decade) than in women (5.