Development of efficient modules for recombinant protein expression and periplasmic localisation in Pseudomonas bharatica CSV86.

Escherichia coli has been widely employed as a host for heterologous protein expression. However, due to certain limitations, alternative hosts like Pseudomonas, Lactococcus and Bacillus are being explored. Pseudomonas bharatica CSV86, a novel soil isolate, preferentially degrades wide range of aromatics over simple carbon sources like glucose and glycerol.

A unique global metabolic trait of CSV86: metabolism of aromatics over simple carbon sources and co-metabolism with organic acids.

Hierarchical utilization of substrate by microbes (utilization of simple carbon sources prior to complex ones) poses a major limitation to the efficient remediation of aromatic pollutants. Aromatic compounds, being complex and reduced in nature, appear to be a deferred choice as the carbon source in the presence of a plethora of simple organic compounds in the environment. The soil bacterium CSV86 displays a unique carbon source utilization hierarchy.