Melatonin synthesis and clock gene regulation in the pineal organ of teleost fish compared to mammals: Similarities and differences.

The pineal organ of all vertebrates synthesizes and secretes melatonin in a rhythmic manner due to the circadian rhythm in the activity of arylalkylamine N-acetyltransferase (AANAT) - the rate-limiting enzyme in melatonin synthesis pathway. Nighttime increase in AANAT activity and melatonin synthesis depends on increased expression of aanat gene (a clock-controlled gene) and/or post-translation modification of AANAT protein. In mammalian and avian species, only one aanat gene is expressed.

Season-dependent effects of photoperiod and temperature on circadian rhythm of arylalkylamine N-acetyltransferase2 gene expression in pineal organ of an air-breathing catfish, Clarias gariepinus.

Arylalkylamine N-acetyltransferase (AANAT) activity, aanat gene expression and melatonin production have been reported to exhibit prominent circadian rhythm in the pineal organ of most species of fish. Three types of aanat genes are expressed in fish, but the fish pineal organ predominantly expresses aanat2 gene. Increase and decrease in daylength is invariably associated with increase and decrease in temperature, respectively.