A thermodynamic study of capillary electrochromatographic retention of aromatic hydrocarbons on a lauryl acrylate porous polymer monolithic column with measured phase ratio.

The phase ratio of a chromatographic system is an important measurement that has long been estimated or calculated, but rarely directly measured. This study utilized a nanoflow liquid chromatography instrument to more accurately measure the phase ratio for a lauryl acrylate porous polymer monolith. Direct measurement of the phase ratio, and its dependence on temperature, allows for a better understanding of the thermodynamics of retention of small analytes.