Discovery of the Lipopeptides Albubactins A-H from RKJM0023 via Chemical Elicitation with Rhamnolipids and Synthesis of Albubactin A.

The marine tunicate-derived RKJM0023 was cultured in the presence of a rhamnolipid mixture in an effort to elicit the production of silent natural products. MS/MS-based molecular networking analysis enhanced with nonparametric statistics highlighted the upregulation of a molecular cluster (Kruskal-Wallis = 1.6 e for ) in which no MS/MS features had library matches.

Discovery of Levesquamide B through Global Natural Product Social Molecular Networking.

Levesquamide A is an isothiazolinone-containing anti-tubercular natural product isolated from sp. RKND-216. Through the use of Global Natural Product Social Molecular Networking (GNPS), additional members of the levesquamide family were identified (B-G).

Microfabrication of a micron-scale microbial-domestication pod for cultivation of marine bacteria.

Through the hyphenation of microfabrication, microfluidics and microbiology, we report the development of a μMicrobial-Domestication Pod (μMD Pod). This cultivation device facilitates cell signaling from neighbouring species and interactions with environmental stimuli for marine bacterial growth to overcome current barriers faced by standard laboratory cultivation methods.

Draft Genome Sequence of sp. Strain RKCA744, Isolated from the Arauca River, Colombia.

sp. strain RKCA744 was isolated from sediment collected from the Arauca River, Colombia.

Draft Genome Sequences of Three Streptomycetes Isolated from Frobisher Bay Marine Sediments.

Three strains (RKAG290, RKAG293, and RKAG337) were isolated from intertidal marine sediments of Frobisher Bay (Canada).

Comparative Microbiome and Metabolome Analyses of the Marine Tunicate from Native and Invaded Habitats.

Massive fouling by the invasive ascidian in Prince Edward Island (PEI, Canada) has been causing devastating losses to the local blue mussel farms. In order to gain first insights into so far unexplored factors that may contribute to the invasiveness of in PEI, we undertook comparative microbiome and metabolome studies on specific tissues from populations collected in invaded (PEI) and native regions (Helgoland and Kiel, Germany). Microbial community analyses and untargeted metabolomics revealed clear location- and tissue-specific patterns showing that biogeography and the sampled tissue shape the microbiome and metabolome of .

Guanahanolide A, a Meroterpenoid with a Sesterterpene Skeleton from Coral-Derived sp.

A meroterpenoid, guanahanolide A (), was purified from a fermentation extract of sp. RKBH-B7. The planar structure of guanahanolide A () was elucidated by NMR spectroscopy, revealing a meroterpenoid comprised of an unprecedented sesterterpene skeleton.

An Ichip-Domesticated Sponge Bacterium Produces an -Acyltyrosine Bearing an α-Methyl Substituent.

The ichip (isolation chip) was employed for the first time in a marine sponge (), and a putatively new bacterial species, sp. RKMC-009, was isolated. Strain RKMC-009 produces a novel -acyltyrosine () that is appended with a rare α-methyl substituent within the aminoacyl moiety and also exhibits Gram-positive antibacterial activity.

Draft Genome Sequence of Streptomyces sp. Strain RKND-216, an Antibiotic Producer Isolated from Marine Sediment in Prince Edward Island, Canada.

sp. strain RKND-216 was isolated from marine sediment collected in Prince Edward Island, Canada, and produces a putatively novel bioactive natural product with antitubercular activity. The genome assembly consists of two contigs covering 5.

Draft Genome Sequence of sp. Strain RKMC-009, Isolated from via Culturing Using an Isolation Chip Diffusion Chamber.

We report the draft whole-genome sequence of sp. strain RKMC-009, which was isolated from the sponge in San Salvador, The Bahamas, using an isolation chip (ichip). Automated biosynthetic gene cluster analysis using antiSMASH 4.