Publications by authors named "Brad Steven Coates"
Article Synopsis
- Diapause is a survival strategy in arthropods that allows them to withstand harsh environmental conditions, with the timing and duration of this process potentially affecting mating cycles across different regions.
- Genetic analysis reveals significant differences in mitochondrial haplotypes between populations that exhibit one or two generations per season, but this difference is closely linked to geographic distance.
- While most genetic variation is low, some specific SNP loci show signs of selection, indicating that distinct mating ecotypes can persist in separate or overlapping areas despite the overall genetic mixing, yet the role of mating generation on this genetic divergence is still unclear.
Microsatellite markers are difficult to apply within lepidopteran studies due to the lack of locus-specific PCR amplification and the high proportion of "null" alleles, such that erroneous estimations of population genetic parameters often result. Herein single nucleotide polymorphism (SNP) markers are developed from Ostrinia nubilalis (Lepidoptera: Crambidae) using next generation expressed sequence tag (EST) data. A total of 2742 SNPs were predicted within a reference assembly of 7414 EST contigs, and a subset of 763 were incorporated into 24 multiplex PCR reactions.
View Article and Find Full Text PDF