Publications by authors named "Brad Bennett"

Article Synopsis
  • * Neutron crystallography (NC) has emerged as a valuable tool in the 21st century for revealing hydrogen atom positions and important water molecules in enzymes, enhancing our understanding of catalytic mechanisms.
  • * This review discusses the advantages of using neutrons in studying enzymes, acknowledges the contributions of Dr. Chris G. Dealwis to the field, and presents examples of successful applications in neutron crystallography research.
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  • A 41-year-old male hiker experienced a serious medical emergency after consuming too much water while hiking in the Grand Canyon during hot weather, leading to multiple health issues.
  • He suffered from exercise-associated hyponatremic encephalopathy (EAHE), exertional rhabdomyolysis (ER), and acute kidney injury (AKI), which resulted in severe pain, disorientation, and a grand mal seizure during helicopter evacuation.
  • The case highlights the dangers of excessive water intake, potential complications from strenuous exercise, and the need for prompt treatment of life-threatening conditions like EAHE and compartment syndromes.
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  • Liposomal bupivacaine is a costly long-acting local anesthetic used in veterinary patients, and multiple uses from a single vial are common to save costs, raising concerns about contamination.
  • This study assessed the safety of multi-dose usage by analyzing samples from the first and last doses for potential bacterial and fungal contamination through various testing methods.
  • Results showed no bacterial growth in cultures, but about 50% of samples had DNA from bacteria or fungi detected; the research concludes that their aseptic handling protocol effectively prevents significant contamination up to 7 uses in 5 days.
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  • Connexins (Cxs) are integral membrane proteins that form hemichannels and gap junctions, enabling communication between cells and their environment, which is vital for development and response to diseases.
  • Abnormal functioning of these channels can lead to various health issues, including inflammation, skin diseases, deafness, and heart problems.
  • Recent studies using high-resolution imaging techniques have revealed detailed structures of Cxs, uncovering mechanisms of channel regulation and potential future research directions to explore their functions in cellular communication.
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  • Prophylactic acetazolamide (ACZ) is commonly used by hikers to prevent acute mountain sickness (AMS), but it carries a risk of acute kidney injury (AKI).
  • A case study presented a 56-year-old man who developed acute exertional rhabdomyolysis and AKI while taking ACZ during a moderate altitude hike in the Grand Canyon.
  • The AKI was linked to rhabdomyolysis and dehydration, highlighting the need for medical providers to carefully consider ACZ's risks and appropriate guidelines for its use in altitude-related activities.
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The Wilderness Medical Society reconvened an expert panel to update best practice guidelines for spinal cord protection during trauma management. This panel, with membership updated in 2023, was charged with the development of evidence-based guidelines for management of the injured or potentially injured spine in wilderness environments. Recommendations are made regarding several parameters related to spinal cord protection.

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  • Dihydrofolate reductase (DHFR) is a key enzyme targeted for antibacterial and antifungal drug development.
  • Eight compounds from the ZINC database were screened virtually to find inhibitors against a specific fungal crop pathogen, with compound Hit8 identified as the most effective DHFR inhibitor.
  • Hit8 demonstrated strong antifungal activity and selectivity, making it a promising candidate for a new fungicide, showcasing the potential of computer-aided drug discovery for agricultural applications.
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  • Connexins (Cx) form gap junction channels (GJCs) by creating hexameric hemichannels that connect adjacent cells, allowing for cell-to-cell communication and independent transport of substances in and out of the cells.
  • A mutation (N176Y) was introduced in Cx26, enabling researchers to analyze the structure of these hemichannels using electron cryomicroscopy, revealing an open pore design similar to GJCs.
  • The study highlights the importance of the conformational flexibility of extracellular loops in recognizing compatible connexin isoforms for effective intercellular docking, while also linking these findings to past research on the function and properties of connexin hemichannels.
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  • Acid-base catalysis is a key chemical mechanism used by enzymes involving proton transfer, but understanding it fully is challenging due to difficulties in observing protons directly.
  • Dihydrofolate reductase (DHFR) serves as a model for this type of catalysis, where it converts dihydrofolate (DHF) into tetrahydrofolate (THF) through hydride transfer and substrate protonation.
  • A recent study using a neutron structure at acidic pH successfully observed the catalytic proton's transfer and proposed a mechanism highlighting the importance of dynamics for solvent entry and substrate protonation via a hydronium ion.
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Course-based undergraduate research experiences (CUREs) often involve a component where the outcomes of student research are broadly relevant to outside stakeholders. We wanted to see if building courses around an environmental justice issue relevant to the local community would impact students' sense of civic engagement and appreciation of the relevance of scientific research to the community. In this quasi-experimental study, we assessed civic engagement and scientific identity gains ( = 98) using pre- and post-semester surveys and open-ended interview responses in three different CUREs taught simultaneously at three different universities.

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As an outcome of combat injury and hemorrhagic shock, trauma-induced hypothermia (TIH) and the associated coagulopathy and acidosis result in significantly increased risk for death. In an effort to manage TIH, the Hypothermia Prevention and Management Kit™ (HPMK) was implemented in 2006 for battlefield casualties. Recent feedback from operational forces indicates that limitations exist in the HPMK to maintain thermal balance in cold environments, due to the lack of insulation.

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Exercise-associated hyponatremia (EAH) is defined by a serum or plasma sodium concentration below the normal reference range of 135 mmol·L that occurs during or up to 24 h after prolonged physical activity. It is reported to occur in individual physical activities or during organized endurance events conducted in environments in which medical care is limited and often not available, and patient evacuation to definitive care is often greatly delayed. Rapid recognition and appropriate treatment are essential in the severe form to increase the likelihood of a positive outcome.

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The Wilderness Medical Society reconvened an expert panel to update best practice guidelines for spinal cord protection during trauma management. This panel, with membership updated in 2018, was charged with the development of evidence-based guidelines for management of the injured or potentially injured spine in wilderness environments. Recommendations are made regarding several parameters related to spinal cord protection.

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To provide guidance to clinicians, the Wilderness Medical Society convened an expert panel to develop evidence-based guidelines for the out-of-hospital evaluation and treatment of victims of accidental hypothermia. The guidelines present the main diagnostic and therapeutic modalities and provide recommendations for the management of hypothermic patients. The panel graded the recommendations based on the quality of supporting evidence and a balance between benefits and risks/burdens according to the criteria published by the American College of Chest Physicians.

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The 2012 study Death on the battlefield (2001-2011) by Eastridge et al.1 demonstrated that 7.5% of the prehospital deaths caused by potentially survivable injuries were due to external hemorrhage from the cervical region.

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Background: Craniomaxillofacial (CMF) injuries are very common in both civilian and military settings. Nearly half of all civilian trauma incidents include a scalp laceration and historical rates of CMF battle injuries increased from 16%-21% to 42.2%.

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Background: Historically, hemorrhage control strategies consisted of manual pressure, pressure dressings, gauze with or without hemostatic ingredients for wound packing, or the use of tourniquets. The iTClamp is a relatively new alternative to stop external bleeding.

Methods: An anonymous survey was used to evaluate the outcomes of the iTClamp in worldwide cases of external bleeding.

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Airway obstruction on the battlefield is most often due to maxillofacial trauma, which may include bleeding and disrupted airway anatomy. In many of these cases, surgical cricothyrotomy (SC) is the preferred airway management procedure. SC is an emergency airway procedure performed when attempts to open an airway using nasal devices, oral devices, or tracheal intubation have failed, or when the risks from intubation are unacceptably high.

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For centuries, cold and wet weather has affected military combat operations leading to tremendous loss of manpower caused by cold-weather-related injuries including trench foot, frostbite, and hypothermia. The initial battlefield management of hypothermia in military personnel had not advanced significantly following many wars and conflicts until 2006. The aim of this review is to: 1) provide an overview of trauma-induced hypothermia (TIH); 2) highlight the Department of Defense strategy for the implementation of a hypothermia clinical management program for battlefield (prehospital) casualties; 3) highlight the research and development of the Hypothermia Prevention and Management Kit (HPMK) as the preferred field rewarming system for battlefield TIH; and 4) emphasize how the HPMK can be easily transitioned to the civilian sector for active rewarming of both accidental and TIH patients.

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Tactical Combat Casualty Care (TCCC) is a set of evidence-based, best-practice prehospital trauma care guidelines customized for use on the battlefield. Military units that have trained all of their unit members in TCCC have now documented the lowest incidence of preventable deaths in the history of modern warfare and TCCC is now the standard for battlefield trauma care in the US Military. TCCC and wilderness medicine share the goal of optimizing care for patients with trauma in austere environments that impose significant challenges in both equipment and evacuation capability.

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Based on lessons learned, many military battlefield trauma advances ultimately transition to enhance civilian trauma care. However, even with major strides to enhance battlefield hemorrhage control, it is unclear how effectively these techniques and products are being translated to civilian trauma. The purpose of this brief review is to present the evidence of current hemostatic product effectiveness, determine the evidence for transitioning of this technology to prehospital civilian application, and provide recommendations about potential use in the wilderness/austere setting.

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