Publications by authors named "Brad A Haltli"

A Gram-negative, motile, rod-shaped marine bacterium, designated RKSG542, was isolated from the sea sponge collected at a depth of 20 m off the west coast of San Salvador, The Bahamas. Phylogenetic analyses based on 16S rRNA gene and genome sequences place RKSG542 in a monophyletic clade with members of the genus . Strain RKSG542 shared <96.

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A Gram-stain-negative, strictly aerobic, motile bacterium, designated strain RKSG073, was isolated from the sea sponge , collected off the west coast of San Salvador, The Bahamas. Cells were curved-to-spiral rods with single, bipolar (amphitrichous) flagella, oxidase- and catalase-positive, non-nitrate-reducing and required salt for growth. RKSG073 grew optimally at 30-37 °C, pH 6-7, and with 2-3 % (w/v) NaCl.

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Two Gram-stain-negative, strictly aerobic, marine bacteria, designated as strains RKSG066 and RKSG123, were isolated from a sponge collected at a depth of 15 m off the west coast of San Salvador, The Bahamas. Investigation of nearly full-length 16S rRNA gene and whole genome-based phylogenies revealed that both strains belong to the order within the class and phylum . Strain RKSG066 formed a monophyletic clade with described members of the genus , while strain RKSG123 formed a well-supported paraphyletic branch apart from this and other related genera within the family .

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A Gram-stain-negative, strictly aerobic, motile, rod-shaped bacterium, designated strain RKSG058, was isolated from the marine sponge Verongula gigantea, collected off the west coast of San Salvador, The Bahamas. Phylogenetic analyses based on 16S rRNA gene sequences revealed that RKSG058 formed a distinct lineage within the family Hahellaceae (order Oceanospirillales, class Gammaproteobacteria), and was most closely related to the genus Endozoicomonas, with sequence similarities to members of this genus ranging from 92.0 to 93.

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The lomaiviticins are a family of cytotoxic marine natural products that have captured the attention of both synthetic and biological chemists due to their intricate molecular scaffolds and potent biological activities. Here we describe the identification of the gene cluster responsible for lomaiviticin biosynthesis in strains DPJ-0016 and DPJ-0019 using a combination of molecular approaches and genome sequencing. The link between the gene cluster and lomaiviticin production was confirmed using bacterial genetics, and subsequent analysis and annotation of this cluster revealed the biosynthetic basis for the core polyketide scaffold.

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