Unraveling the multiple facilitative effects of consumers on marine primary producers.

The loss of consumers threatens the integrity of ecological systems, but the mechanisms underlying the effects on communities and ecosystems remain difficult to predict. This is, in part, due to the complex roles that consumers play in those systems. Here, we highlight this complexity by quantifying two mechanisms by which molluscan grazers-typically thought of as consumers of their algal resources-facilitate algae on rocky shores.

Field Collection and Laboratory Maintenance of Canopy-Forming Giant Kelp to Facilitate Restoration.

Canopy-forming kelps are essential foundation species, supporting biodiversity and providing ecosystem services valued at more than USD$500 billion annually. The global decline of giant kelp forests due to climate-driven ecological stressors underscores the need for innovative restoration strategies. An emerging restoration technique known as 'green gravel' aims to seed young kelps over large areas without extensive underwater labor and represents a promising restoration tool due to cost-effectiveness and scalability.

Spectroscopic and Computational pH Study of Ni and Pd Pyrrole-Imine Chelates with Human Serum Albumin.

Human serum albumin (HSA) efficiently transports drugs in vivo: most are organic. Therefore, it is important to delineate the binding of small molecules to HSA. Here, for the first time, we show that HSA binding depends not only on the identity of the d metal ion, Ni or Pd, of their complexes with bis(pyrrole-imine), HPrPyrr, but on the pH level as well.

The functional effects of a dominant consumer are altered following the loss of a dominant producer.

Human impacts on ecosystems are resulting in unprecedented rates of biodiversity loss worldwide. The loss of species results in the loss of the multiple roles that each species plays or functions (i.e.

Biological modification of coastal pH depends on community composition and time.

Biological processes play important roles in determining how global changes manifest at local scales. Primary producers can absorb increased CO via daytime photosynthesis, modifying pH in aquatic ecosystems. Yet producers and consumers also increase CO via respiration.

Climate Change Amelioration by Marine Producers: Does Dominance Predict Impact?

AbstractClimate change threatens biodiversity worldwide, and assessing how those changes will impact communities will be critical for conservation. Dominant primary producers can alter local-scale environmental conditions, reducing temperature shading and mitigating ocean acidification photosynthesis, which could buffer communities from the impacts of climate change. We conducted two experiments on the coast of southeastern Alaska to assess the effects of a common seaweed species, , on temperature and pH in field tide pools and tide pool mesocosms.

Childhood asthma and household exposures to nitrogen dioxide and fine particles: a triple-crossover randomized intervention trial.

Objective: Triple-crossover randomized controlled intervention trial to test whether reduced exposure to household NO or fine particles results in reduced symptoms among children with persistent asthma.

Methods: Children ( = 126) aged 5-11 years with persistent asthma living in homes with gas stoves and levels of NO 15 ppb or greater recruited in Connecticut and Massachusetts (2015-2019) participated in an intervention involving three air cleaners configured for: (1) NO reduction: particle filtration and NO scrubbing; (2) particle filtration: HEPA filter and NO scrubbing; (3) control: particle filtration and NO scrubbing. Air cleaners were randomly assigned for 5-week treatment periods using a three-arm crossover design.

Accounting for variation in temperature and oxygen availability when quantifying marine ecosystem metabolism.

It is critical to understand how human modifications of Earth's ecosystems are influencing ecosystem functioning, including net and gross community production (NCP and GCP, respectively) and community respiration (CR). These responses are often estimated by measuring oxygen production in the light (NCP) and consumption in the dark (CR), which can then be combined to estimate GCP. However, the method used to create "dark" conditions-either experimental darkening during the day or taking measurements at night-could result in different estimates of respiration and production, potentially affecting our ability to make integrative predictions.

Training behavioural therapists in presession pairing skills to evaluate the impact on children's life skill acquisition rates.

Presession pairing is a well-documented behaviour analytic practice that supports the development of a positive instructional environment and is associated with reduced rates of challenging behaviour among children with developmental disabilities. However, there is limited research evaluating the impact of presession pairing on children's skill acquisition rates. Therefore, in the current study, four behavioural therapists were systematically trained in an established presession pairing protocol and using a multiple baseline across participants design, the impact of this training on life skill acquisition rates among a group of four children with Autism Spectrum Disorder was evaluated.

ZBTB2 represses HIV-1 transcription and is regulated by HIV-1 Vpr and cellular DNA damage responses.

Previously, we reported that cellular transcription factor ZASC1 facilitates DNA-dependent/RNA-independent recruitment of HIV-1 TAT and the cellular elongation factor P-TEFb to the HIV-1 promoter and is a critical factor in regulating HIV-1 transcriptional elongation (PLoS Path e1003712). Here we report that cellular transcription factor ZBTB2 is a novel repressor of HIV-1 gene expression. ZBTB2 strongly co-immunoprecipitated with ZASC1 and was dramatically relocalized by ZASC1 from the cytoplasm to the nucleus.

Intertidal Canopy-forming Seaweeds Modulate Understory Seaweed Photoprotective Compounds.

Foundation species provide physical structure that enhances the diversity and abundance of associated organisms. Canopy-forming seaweeds are known to act as foundation species on rocky shores by lowering temperature and desiccation stress. Direct solar radiation, including ultraviolet (UV) light, can also reduce photosynthetic rates in algae, cause oxidative stress and DNA damage.

Flexibility of nutritional strategies within a mutualism: food availability affects algal symbiont productivity in two congeneric sea anemone species.

Mutualistic symbioses are common, especially in nutrient-poor environments where an association between hosts and symbionts can allow the symbiotic partners to persist and collectively out-compete non-symbiotic species. Usually these mutualisms are built on an intimate transfer of energy and nutrients (e.g.

Interest in Digital Dietary Support Among Adults With Kidney Failure Receiving Hemodialysis.

Objective: This study assessed interest in digital dietary support among adults with kidney failure receiving hemodialysis.

Methods: We recruited English-speaking adults with kidney failure receiving hemodialysis 2+ times per week online and in dialysis clinics to complete a brief survey. Participants were asked if they were interested in participating in an online patient community with access to a registered dietitian (RD) (not at all, slightly, somewhat, very, or extremely interested).

Self-Efficacy and Nutrition-Related Goal Achievement of MyFitnessPal Users.

Mobile health applications (apps) are increasing in popularity to assist in health behavior change and maintenance. Drawing from constructs outlined within the social cognitive theory by Bandura, we explored how frequently users of a popular health app achieved their health-related goals. Participants reportedly used the MyFitnessPal (MFP) app within the past year completed an online survey on how often they used MFP, their self-efficacy for healthy eating (SE-HE), and achievement of nutrition-related goals.

Complementarity in spatial subsidies of carbon associated with resource partitioning along multiple niche axes.

The co-occurrence of several species, all of which share similar resource requirements, remains a paradox in ecology. Here, I evaluated resource use along multiple environmental axes to understand the potential for niche partitioning and complementarity in a guild of suspension-feeding rocky shore invertebrates. I focused on the mussels Perna canaliculus, Mytilus galloprovincialis, Aulacomya maoriana, and Xenostrobus pulex, all of which coexist along the coastline of the South Island of New Zealand.

Interactive effects of large- and local-scale environmental gradients on phenotypic differentiation.

Intraspecific differentiation across a steep environmental gradient depends on the relative influences of evolutionary, organismal, and environmental processes. But steep environmental gradients may be nested within larger-scale, regional conditions that could influence these processes at the local scale. Therefore, we hypothesized that phenotypic differentiation along a steep environmental gradient would vary among regions.

Pulmonary and cardiovascular safety of inhaled insulin in routine practice: The Exubera Large Simple Trial (VOLUME).

Objective: VOLUME is a randomized, open-label, post-approval pragmatic trial aiming to evaluate long-term pulmonary and cardiovascular safety of Exubera® (EXU; insulin human [rDNA origin] Inhalation Powder) in routine clinical practice. The primary study objective is to compare risk of persistent decline in forced expiratory volume in 1 second (FEV) among patients treated with and without EXU.

Research Design And Methods: Patients eligible to take EXU per approved local label were randomized to EXU or routine care and followed per usual care, with scheduled FEV tests at baseline, 6 months, and yearly.

Updating insights into rosiglitazone and cardiovascular risk through shared data: individual patient and summary level meta-analyses.

Objectives: To conduct a systematic review and meta-analysis of the effects of rosiglitazone treatment on cardiovascular risk and mortality using multiple data sources and varying analytical approaches with three aims in mind: to clarify uncertainties about the cardiovascular risk of rosiglitazone; to determine whether different analytical approaches are likely to alter the conclusions of adverse event meta-analyses; and to inform efforts to promote clinical trial transparency and data sharing.

Design: Systematic review and meta-analysis of randomized controlled trials.

Data Sources: GlaxoSmithKline's (GSK's) ClinicalStudyDataRequest.

Implications of product withdrawal on a post-approval pragmatic trial: The VOLUME study experience.

Introduction: Many clinical trials terminate early due to safety and efficacy concerns, and less often due to unexpected "positive" findings. However, early termination of post-approval (Phase IV) pragmatic randomized trials for commercial reasons is less frequent, may be more complex, and may require added flexibility in closure methods, including short term follow-up. VOLUME was a randomized, open-label, post-approval pragmatic clinical trial (PCT) or large simple trial that terminated early due to product withdrawal.

Delivering a Post-Partum Weight Loss Intervention via Facebook or In-Person Groups: Protocol for a Randomized Feasibility Pilot Trial.

Background: Postpartum weight retention contributes to long-term weight gain and obesity for many women. Lifestyle interventions with numerous visits are logistically challenging for many postpartum women. Delivering a lifestyle intervention via social media may overcome logistic challenges to participation in in-person weight loss programs.

Lung Cancer-Related Mortality With Inhaled Insulin or a Comparator: Follow-Up Study of patients previously enrolled in Exubera Controlled Clinical Trials (FUSE) Final Results.

Objective: The Follow-Up Study of patients previously enrolled in Exubera controlled clinical trials (FUSE) was designed to evaluate whether patients previously treated with Exubera (EXU; insulin human [rDNA origin], inhaled powder) in controlled clinical trials died because of incident primary lung cancer at a substantially higher rate than patients treated with a comparator.

Research Design And Methods: FUSE is a hybrid, randomized, controlled trial/cohort study including participants of 17 prior EXU clinical trials. Pooled patient data from these trials were used, and the subset of patients enrolled in the follow-up cohort study was followed prospectively for 2 years in order to evaluate the incidence of fatal and nonfatal primary lung cancers and all-cause mortality.