Publications by authors named "Bozidar Sarler"

The results of an experimental study of micro-jets produced with a gas dynamic virtual nozzle (GDVN) under the influence of an electric field are provided and discussed for the first time. The experimental study is performed with a 50% volume mixture of water and ethanol, and nitrogen focusing gas. The liquid sample and gas Reynolds numbers range from 0.

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The purpose of the present paper is to predict the grain size of steel during the hot-rolling process. The basis represents a macroscopic simulation system that can cope with temperatures, stresses and strains of steel in a complete continuous rolling mill, including reversible pre-rolling and finishing rolling with several tenths of rolling passes. The grain size models, newly introduced in the present paper, are one-way coupled to the macro-scale calculations performed with the slice model assumption.

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The purpose of this work is to determine, based on the computational model, whether a mixture of a binary liquid is capable of producing longer, thinner and faster gas-focused micro-jets, compared to the mono-constituent liquids of its components. Mixtures of water with two different alcohols, water + ethanol and water + 2-propanol, are considered. The numerical study of pre-mixed liquids is performed in the double flow focusing nozzle geometry used in sample delivery in serial femtosecond crystallography experiments.

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Liquid micro-jets are crucial for sample delivery of protein crystals and other macromolecular samples in serial femtosecond crystallography. When combined with MHz repetition rate sources, such as the European X-ray free-electron laser (EuXFEL) facility, it is important that the diffraction patterns are collected before the samples are damaged. This requires extremely thin and very fast jets.

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Two binary biodegradable Mg-alloys and one ternary biodegradable Mg-alloy (Mg-0.3Ca, Mg-5Zn and Mg-5Zn-0.3Ca, all in wt%) were investigated.

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Article Synopsis
  • Serial femtosecond crystallography relies on efficient delivery of fresh crystals to an X-ray free-electron laser during experiments.
  • A new double-flow focusing nozzle has been developed that reduces sample consumption and enhances jet stability compared to earlier models.
  • This nozzle successfully determined the first room-temperature structure of RNA polymerase II and was tested on other protein samples, including an extradiol ring-cleaving dioxygenase.
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