Light-Emitting-Diode-Induced Fluorescence from Organic Dyes for Application in Excitation-Emission Fluorescence Spectroscopy for Food System Analysis.

An experimental study is presented on the possibility of using the fluorescence from organic dyes as a broadband light source together with a monochromator for applications in excitation-emission matrix (EEM) fluorescence spectroscopy. A high-power single-chip light-emitting diode (LED) was chosen as an excitation source with a central output wavelength at 365 nm to excite a fluorescent solution of Coumarin 1 dye dissolved in ethanol. Two excitation configurations were investigated: direct excitation from the LED and excitation through an optical-fiber-coupled LED.

A Magnetoelectric Automotive Crankshaft Position Sensor.

The paper is devoted to the possibility of using magnetoelectric materials for the production of a crankshaft position sensor for automobiles. The composite structure, consisting of a PZT or LiNbO piezoelectric with a size of 20 mm × 5 mm × 0.5 mm, and plates of the magnetostrictive material Metglas of the appropriate size were used as a sensitive element.

Pathogenicity of avian myelocytomatosis virus MC29 in two lines of chickens.

The pathogenicity of avian myelocytomatosis virus MC29 was studied in F1 hybrids of two Prague inbred lines of chickens-CC xIA (B4/B7 genotype) and CB x IA (B12/B7 genotype). A shorter latent period and a higher mortality was found in the CC x IA chickens. In most chickens of this group, subperiosteal accumulations of primitive cells on the ribs, sternum and pelvic bones were observed, which were never detected in the CB x IA chickens.

Protection against bovine leukemia virus infection in two breeds of cattle.

Calves of two breeds--Brown Latvian and Black and White, were immunized against bovine leukemia virus with an immunogen produced from short-term cultured blood lymphocytes of BLV-positive Brown Latvian cows. The immunogen contained the viral proteins gp51 and p24 as well as some cellular proteins. As determined by bioassay in sheep, it did not contain infective bovine leukemia virus.

Electron- and immunoelectron-microscopic investigation on the rabbit haemorrhagic disease virus.

Electron- and immunoelectron-microscopic methods were used for detection and investigation of rabbit haemorrhagic disease virus (RHDV). The observed virus particles were uncoated, with an icosahedral symmetry, with a 35-40 nm dia and capsids built up by 32 capsomers with a central hollow part and a 3.5-5 nm dia.

Immunohistochemical localization of the rabbit haemorrhagic disease viral antigen.

Immunohistochemical investigations were carried out to determine organ and cellular localization of the rabbit haemorrhagic disease viral antigen (RHDVA). It was found in certain parenchymal liver cells near the interlobular septs and in some macrophages and pseudoeosinophils of all studied organs and blood. Whereas in morphologically preserved hepatocytes and macrophages the RHDVA accumulated in the nuclei, in cells with disintegrated nuclei it was distributed throughout the cytoplasm.

Haematological and pathomorphological studies on chickens inoculated with transformation-defective sarcoma virus.

A transformation-defective mutant of Rous sarcoma virus (td PR-RSV-C) inoculated into chicken embryos caused a number of pathological changes in chickens after hatching. Disturbances in haematopoiesis were manifested by anaemia of different degree and by the presence of immature blood elements in the peripheral blood. The haematological picture of erythroblastosis was confirmed histologically.

[Electron microscopy research on cytopathic rotaviruses in cell cultures].

Studied was the virus reproduction in cell cultures of MA-104 of the referent rotavirus strain Lincoln, and of the isolated from calves rotavirus Trebich 248/82 and Dolno-sakhrane 39/82. Observed were the following main morphologic structures: electron-dense oval fractions, fractions of with single and double membrane, fractions with supercapsular membrane and electronic-densely granulated zones--viroplasms. There were not established considerable differences between the referent and the isolated from calves rotavirus strains in our country.

[Parallel morphological, enzyme and immunological research on the jejunal mucosa in patients with gluten enteropathy, chronic nonspecific enteritis and ulcerative colitis].

The results are reported from the study on 120 patients, 30 of them with gluten enteropathy, 50--with chronic unspecified enteritis and 40--with chronic ulcerous colitis. Small intestinal aspiration biopsy was performed to all patients with histomorphological, electron microscopic, enzymatic and immune-fluorescent study. The secretory immunoglobulins in the jejunal juice were studied in 40 patients.

[Electron microscopic research on an experimental infection in chicks caused by the infectious bursal virus].

A total of 18 birds at the age of 25 days were infected intraocularly with the pathogenic strain 52/70 of the infectious bursitis virus. The bursa of Fabricius and the thymus were sampled for electron-microscopic investigations. The ultrastructural changes in the infected cells consisted in pycnosis of the lymphocyte and macrophage nuclei, vacuolization of the cytoplasm, and the production of lipid droplets as well as of inclusion bodies of varying density, myelin figures, multivericular bodies, and other irregular structures in the cytoplasm.

[Changes in the cAMP content in the intestinal and liver tissue and in 35S-thiamine transport in chickens fed a low-protein diet].

An experiment was carried out with 45 male broiler birds divided into three groups: I (controls), given a full-diet ration with 22.16 per cent protein; II, given a ration with 18.12 per cent protein; and III, fed a ration with 14.

[Whipple disease: clinical and immunohistologic findings in 2 cases].

Two cases with typical clinical and histological manifestations of Whipple's disease were reported. Peroral jejunal biopsies were studied in the florid stage and during remission achieved by tetracycline therapy. A complete absence of immunoglobulin-containing cells (Ig-CC) and an increased number of mast cells in the mucosa were established in one of the cases.

[Ultrastructural changes in the resorptive epithelial cells of the small intestine in rats during increased motor activity and immobilization].

Investigated were the structural changes in the epithelial resorptive cells of the duodenum and the jejunum of rats in the conditions of continuous and varying locomotor activity--intense locomotion, control, and immobilization. It was established that the microvilli in the immobilized animals were of a lower size, were irregularly distributed and lower in number. The inward bending of the lateral cell boundary was more slightly manifested.

Hepatoprotective effect of silymarin (carsil) on liver of D-galactosamine treated rats. Biochemical and morphological investigations.

The hepatoprotective effect of carsil (generic name silymarin) on a model of liver intoxication with D-galactosamine in rats is studied. The changes in the activity of the serum enzymes GOT, GPT, MDH, SDH, ICDH, AP. AhE and the total protein as well as the UDP-sugars content in the liver is investigated.

[Ultrastructural changes in the red bone marrow in hyper- and hypokinesia].

Changes were looked for in the bone marrow of rats, put for a long period of ontogenesis under different degrees of muscular activity. The experiment was carried out on three groups of animals of the same age--those for the purpose of control, those loaded with movements, and those immobilized. The ultrastructural picture of the bone marrow with the rats loaded with movements showed an active hematopoiesis which was especially pronounced in the erythrocyte row.