Effect of Prestrain on Payne Effect and Hysteresis Loss of Carbon-Black-Filled Rubber Vulcanizates: Measurements and Modeling.

The performance of a viscoelastic damper is governed by the mechanical properties of the viscoelastic material, which are sensitive to prestrain. Among viscoelastic materials, carbon black (CB)-filled rubber vulcanizate is commonly used in structural applications. In this paper, the prestrain-dependent Payne effect and hysteresis loss of CB-filled rubber vulcanizates are investigated through experimental and theoretical analysis.

A Nonlinear Fractional Viscoelastic-Plastic Creep Model of Asphalt Mixture.

The mechanical behavior of asphalt mixture under high stresses presents nonlinear viscoelasticity and permanent deformation. In this paper, a nonlinear fractional viscoelastic plastic (NFVEP) creep model for asphalt mixture is proposed based on the Nishihara model, with a Koeller spring-pot replacing the Newton dashpot. The NFVEP model considers the instantaneous elasticity, viscoelasticity with damage and time-hardening viscoplasticity with damage concurrently, and the viscoelastic response is modeled by fractional derivative viscoelasticity.

Fatigue Life Assessment of Filled Rubber by Hysteresis Induced Self-Heating Temperature.

As a viscohyperelastic material, filled rubber is widely used as a damping element in mechanical engineering and vehicle engineering. Academic and industrial researchers commonly need to evaluate the fatigue life of these rubber components under cyclic load, quickly and efficiently. The currently used method for fatigue life evaluation is based on the S-N curve, which requires very long and costly fatigue tests.

Effect of Temperature on the Tear Fracture and Fatigue Life of Carbon-Black-Filled Rubber.

The mechanical behaviour of carbon-black (CB)-filled rubber is temperature-dependent. It is assumed that temperature affects the fatigue life of rubber products by changing the tear energy of the material. The static tearing behaviour and fatigue crack propagation behavior of CB-filled rubber at different temperatures were investigated in this study.

Developing a genetically encoded green fluorescent protein mutant for sensitive light-up fluorescent sensing and cellular imaging of Hg(II).

Hg(II) is well-known for quenching fluorescence in a distance dependent manner. Nevertheless, when we exposed the fluorophore of a green fluorescent protein (GFP) toward Hg(II), through H148C mutation, the GFP fluorescence could be "lighted up" by Hg(II) down to sub-nM level. The detection linear range is 0.

Mimotope ELISA for detection of broad spectrum antibody against avian H5N1 influenza virus.

Background: We have raised a panel of broad spectrum neutralizing monoclonal antibodies against the highly pathogenic H5N1 avian influenza virus, which neutralize the infectivity of, and afford protection against infection by, most of the major genetic groups of the virus evolved since 1997. Peptide mimics reactive with one of these broad spectrum H5N1 neutralizing antibodies, 8H5, were identified from random phage display libraries.

Method: The amino acid residues of the most reactive 12mer peptide, p125 (DTPLTTAALRLV), were randomly substituted to improve its mimicry of the natural 8H5 epitope.

Induction of cross-reactive antibodies against mimotopes of H5N1 hemagglutinin.

A sub-library based on peptide mimic 125 was designed and constructed, and 18 phagotopes specifically binding 8H5mAb were isolated. Antisera against three phagotopes, containing peptide 12MH-1, 12MH-5 and 12MH-8 reacted with 3 different H5N1 virus strains, but not with 2 H1N1 and 2 H3N2 viruses by Dot blots. The affinity of 12MH-8 was approximately eight times more than 12MH-1 or 12MH-5 or parent peptide 125.