Dispersive solid-phase extraction based on zirconium metal-organic framework coupled with gas chromatography-mass spectrometry for determining sugar phosphates in biological samples.

Background: Sugar phosphates (SPx) play important role in the metabolism of the organism. SPx such as glycerate 3-phosphate, fructose 6-phosphate and glucose 6-phosphate in biological samples have the poor stability, similar structure and low abundance, which make their separation and detection more challenging.

Method: UiO-66-NH and ZrO coated SiO(SBA-15) hard-core-shell adsorbents (UiO-66-NH@SBA-15 and ZrO@SBA-15) were synthesized, which were further used for dispersive solid-phase extraction for enriching the SPx in biological samples.

[Comparison of the effects between maize germ oil and lard oil loading on blood lipids and fatty acid profile of mice].

Objective: To investigate the changes in blood lipids and fatty acids profile of mice after different oil loading, and explore the effects of different dietary fatty acids on postprandial blood lipids and fatty acid profile.

Methods: Ninety-six C57BL/6 mice were randomly divided into 2 groups by weight ,maize germ oil group and lard oil group. The mice were given maize germ oil or lard oil by gavage at a dose of 1 ml/100 mg BW, respectively, after over-night fasting.