Behavioral Responses of Larvae as Proxies for Cyanobacterial Metabolite Interactions: Insights from Ternary Combinations.

This study aimed to assess the behavioral responses (immobilization, horizontal and vertical motility, and response to light) of larvae exposed to individual cyanobacterial metabolites aeruginosin 98B (AER-B), anabaenopeptin-B (ANA-B), and cylindrospermopsin (CYL), and their binary and ternary mixtures. The investigation revealed that single metabolites ANA-B and CYL exhibited the highest potency in immobilizing the larvae. Notably, the binary mixture AER-B+CYL induced a remarkably strong synergistic interaction, while other tested binary and ternary mixtures demonstrated antagonistic effects.

Responses of RTgill-W1 cells to cyanobacterial metabolites microcystin-LR, anabaenopeptin-A, cylindrospermopsin, their binary and ternary mixtures.

The aim of our study was to investigate the effects of cyanobacterial metabolites: microcystin-LR (MC-LR) anabaenopeptin-A (ANA-A), cylindrospermopsin (CYL), their binary and ternary mixtures on rainbow trout (Oncorhynchus mykiss) gill (RTgill-W1) cell line. We determined the following cell parameters: Hoechst and propidium iodide (PI) double staining, intracellular ATP level with luminometric assay, glutathione level with ThiolTracker Violet®- glutathione detection reagent and cytoskeletal F-actin fluorescence. The results showed that although reduction of Hoechst fluorescence was observed in both binary and ternary combinations of cyanobacterial metabolites, the mixture of MC-LR + ANA-A + CYL was the most potent inhibitor (EC50 = 148 nM).

First report on adverse effects of cyanobacterial anabaenopeptins, aeruginosins, microginin and their mixtures with microcystin and cylindrospermopsin on aquatic plant physiology: An experimental approach.

Cyanobacteria produce a variety of oligopeptides beyond microcystins and other metabolites. Their biological activities are not fully recognized especially to aquatic plants. Acute toxicity tests on Spirodela polyrhiza and Lemna minor exposed to a range of concentrations of cyanobacterial metabolites: anabaenopeptins (ANA-A, ANA-B), aeruginosins 98 (Aer-A, Aer-B), microginin-FR1 (MG-FR1), microcystin-LR (MC-LR) and cylindrospermopsin (Cyl) were carried out to compare their influence on plant physiology.

Cyanobacterial metabolites: Aeruginosin 98A, microginin-FR1, anabaenopeptin-A, cylindrospermopsin and their mixtures affect behavioral and physiological responses of Daphnia magna.

We determined the effects influence of cyanobacterial products metabolites: aeruginosin-A (AER-A), microginin-FR1 (MG-FR1), anabaenopeptin-A (ANA-A), cylindrospermopsin (CYL) and their binary and quadruple mixtures on swimming behavior, heart rate, thoracic limb activity, oxygen consumption and in vivo cell health of Daphnia magna. The study showed that CYL induced mortality of daphnids at the highest concentrations, however three oligopeptides had no lethal effect. All the tested Each single metabolites inhibited swimming speed.

Effects of cyanobacterial metabolites: Aeruginosin 98A, microginin-FR1, anabaenopeptin-A, cylindrospermopsin in binary and quadruple mixtures on the survival and oxidative stress biomarkers of Daphnia magna.

The aim of our study was to determine the effects of aeruginosin 98 A (ARE-A), microginin-FR1 (MG-FR1), anabaenopeptin-A (ANA-A) cylindrospermopsin (CYL) and their binary and quadruple mixtures on the survival and the levels of oxidative stress biomarkers in Daphnia magna: total glutathione (GSH), catalase (CAT), dismutase (SOD) and malondialdehyde (MDA). The biochemical indicators were measured with ELISA kits and the interactive effects were determined by isobole and polygonal analysis with Compusyn® computer software. The study revealed that oligopeptides did not decrease daphnid survival, only CYL inhibited this parameter, with synergistic effects when it was used as a component.

Behavioral disturbances induced by cyanobacterial oligopeptides microginin-FR1, anabaenopeptin-A and microcystin-LR are associated with neuromotoric and cytotoxic changes in Brachionus calyciflorus.

Aquatic animals are exposed to various cyanobacterial products released concomitantly to the environment by decaying blooms. Although there exist results on the toxicity of cyanobacterial extracts little is known on the influence of pure oligopeptides or their mixtures and elucidated mechanisms of behavioral toxicity in zooplanktonic organisms. Therefore, the aim of the present study was to assess the effects of single and mixed pure cyanobacterial oligopeptides: microginin FR-1 (MG-FR1), anabaenopeptin-A (ANA-A) and microcystin-LR (MC-LR) at various concentrations on the swimming behavior and catecholamine neurotransmitter activity, muscular F-actin structure, DNA nuclear content and cell viability of a model rotifer Brachionus calyciflorus.

Beyond the behavioural phenotype: Uncovering mechanistic foundations in aquatic eco-neurotoxicology.

During the last decade, there has been an increase in awareness of how anthropogenic pollution can alter behavioural traits of diverse aquatic organisms. Apart from understanding profound ecological implications, alterations in neuro-behavioural indices have emerged as sensitive and physiologically integrative endpoints in chemical risk assessment. Accordingly, behavioural ecotoxicology and broader eco-neurotoxicology are becoming increasingly popular fields of research that span a plethora of fundamental laboratory experimentations as well as applied field-based studies.

Synergistic toxicity of some cyanobacterial oligopeptides to physiological activities of Daphnia magna (Crustacea).

Anabaenopeptins and microcystins are oligopeptides produced by bloom-forming cyanobacteria. We determined in vivo effects of anabaenopeptin-B (AN-B) and two variants of microcystins of different hydrophobicity (MC-LR and MC-LF) on the physiology of Daphnia magna. Heart rate, thoracic limb activity and post-abdominal claw activity were determined by digital video analysis and oxygen consumption by Oxygraph + system.

Advances in real-time monitoring of water quality using automated analysis of animal behaviour.

Monitoring of freshwater quality and its potential sudden contamination is integral to human health, sustainable economic development and prediction of pollutant impact on aquatic ecosystems. Although there have been significant advances in technologies for automated sampling and continuous analysis of water physicochemical parameters, the current capabilities for real-time warning against rapidly developing unknown mixtures of chemical hazards are still limited. Conventional chemical analysis systems are not suitable for assessing unknown mixtures of chemicals as well as additive and/or synergetic effects on biological systems.

Cyanobacterial anabaenopeptin-B, microcystins and their mixture cause toxic effects on the behavior of the freshwater crustacean Daphnia magna (Cladocera).

Comparison of the toxic effects caused by the pure cyanobacterial cyclic hexapeptide anabaenopeptin-B (AN-B), the heptapeptides: microcystin-LR (MC-LR) and MC-LF as well as a binary mixture of AN-B with MC-LR on the swimming speed and hopping frequency - essential activities of Daphnia, was experimentally determined. Till now, no information on behavioral effects of AN-B and its mixture with microcystins, commonly produced by cyanobacteria, was available. Also MC-LF effect on aquatic crustaceans was determined for the first time.

Applications of advanced neuro-behavioral analysis strategies in aquatic ecotoxicology.

Despite mounting evidence of pleiotropic ecological risks, the understanding of the eco-neurotoxic impact of most industrially relevant chemicals is still very limited. In particularly the acute and chronic exposures to industrial pollutants on nervous systems and thus potential alterations in ecological fitness remain profoundly understudied. Since the behavioral phenotype is the highest-level and functional manifestation of integrated neurological functions, the alterations in neuro-behavioral traits have been postulated as very sensitive and physiologically integrative endpoints to assess eco-neurotoxicological risks associated with industrial pollutants.

Short-term effects of pesticide fipronil on behavioral and physiological endpoints of Daphnia magna.

Fipronil (FIP) is an organic pesticide with many practical uses. Although some results indicated toxic effects in some terrestrial and aquatic animal species, little is known on its influence on behavioral and physiological endpoints of cladocerans. The aim of our study was to determine the short-term effects of FIP at concentrations of 0.

Effects of L-proline on cellular responses of hen erythrocytes subjected to thermal stress.

Little is known on the protective effects of L-proline on hen erythrocytes. The aim of the study was to determine the protective effects of this amino acid at concentrations of 50 μg/mL, 100 μg/mL, 200 μg/mL in hen erythrocytes subjected to temperatures 41 °C, 43 °C and 45 °C for 1 h and 4 h. The following cellular parameters were determined: viability, morphological alterations, caspase 3/7 activity, heat shock protein HSP70 1A activity and glutathione level.

Behavioral and physiological responses of Daphnia magna to salicylic acid.

Salicylic acid (SA), a metabolite of acetylsalicylic acid is a monohydroxybenzoic acid a common non-steroidal analgesic and anti-inflammatory drug (NSAID) frequently detected in various aquatic ecosystems at concentrations up to 19.50 μg L in surface waters near livestock farms and 59.6 μg L in wastewaters.

Identification of Mitochondrial DNA (NUMTs) in the Nuclear Genome of .

This is the first study in which the () nuclear genome (nDNA) obtained from the GenBank database was analyzed for pseudogene sequences of mitochondrial origin. To date, there is no information about pseudogenes localized in genome. This study aimed to identify NUMTs, their length, homology, and location for potential use in evolutionary studies and to check whether their occurrence causes co-amplification during mitochondrial genome (mtDNA) analyses.

Daphnia magna model in the toxicity assessment of pharmaceuticals: A review.

Daphnia magna is one of the most commonly used model organism to assess toxicity of wide range of pharmaceuticals such as antibiotics, anticancer drugs, antidepressants, anti-inflammatory drugs, beta-blockers and lipid-regulating agents. Currently, daphnia toxicity tests based on immobilisation and lethality standardised by OECD, acute immobilisation test and reproduction test, are mainly used in toxicological studies. Detailed analysis of Daphnia biology allows distinguishing the swimming behaviour and physiological endpoints such as swimming speed, distance travelled, hopping frequency, heart rate, ingestion rate, feeding rate, oxygen consumption, thoracic limb activity which could be also useful in assessment of toxic effects.

Early morphological and apoptotic responses of bird erythrocytes to thermal stress.

Little is known about early indicators of heat stress in bird erythrocytes. We investigated the effects of elevated temperatures on the morphology and cellular responses of avian erythrocytes. Hen red blood cells were subjected to 22-45 °C temperatures for 1 h and 4 h, then stained and examined by light microscopy to assess morphological alterations.

Ketoprofen affects swimming behavior and impairs physiological endpoints of Daphnia magna.

Ketoprofen (KET) is a nonsteroidal anti-inflammatory and analgesic drug commonly used in human and veterinary medicine. This compound is detected in aquatic reservoirs however, little is known about its influence on cladocerans. Therefore, the aim of our study was to determine the influence of KET at concentrations of 0.

Early indicators of behavioral and physiological disturbances in Daphnia magna (Cladocera) induced by cyanobacterial neurotoxin anatoxin-a.

The majority of reports on the toxic effect of cyanobacterial metabolites on the freshwater invertebrates is based on determination of two endpoints: mortality or immobilization. However, detection of sub-lethal effects requires more sensitive indicators The aim of the present study was to evaluate the applicability of digital-video analysis for determination of early behavioral and physiological responses in the assessment of effects caused by the cyanobacterial neurotoxin, anatoxin-a (ANTX) at a broad range of its concentration (0.5-50 μg/mL).

Cisplatin affects locomotor activity and physiological endpoints of Daphnia magna.

Cisplatin (CPL) is a common antineoplastic drug used in human medicine for treatment of various cancer types. Since the knowledge about its effects on crustacean behavioral and physiological parameters is very scarce, the aim of our study was to determine the influence of CPL at concentrations of 125 μg/L, 200 μg/L, 500 μg/L and 1000 μg/L on swimming behavior (swimming speed, distance travelled, hopping frequency, propelling efficiency index - a novel parameter) and physiological parameters (heart rate, thoracic limb activity) of Daphnia magna with the use of video digital analysis. The results showed that distance travelled, swimming speed, hopping frequency and propelling efficiency were inhibited as early as after 24 h in concentration- and time-dependent manner.

Physiological endpoints in daphnid acute toxicity tests.

Daphnids are freshwater crustaceans used in toxicity tests. Although lethality and immobilisation are the most commonly used endpoints in those tests, more sensitive parameters are required for determination of sublethal acute effects of toxicants. The use of various physiological endpoints in daphnids is considered as a low-cost and simple alternative that meets the 3R's rule (Replacement, Reduction, Refinement) criteria.

Effects of L-proline on swimming parameters of Daphnia magna subjected to heat stress.

L-proline (L-PROL) is an essential amino acid, a constituent of many proteins and the osmoprotective molecule produced and accumulated in higher plants and some freshwater microalgae in response to various environmental stressors. Knowledge on thermoprotective effects of this amino acid on freshwater invertebrates is very scarce. Therefore the aim of our study was to determine the effect of L-PROL at concentrations: 10 mg/L, 20 mg/L and 50 mg/L on swimming behavior (immobilization, swimming track density, swimming speed, turning ability) of Daphnia magna subjected to temperatures: 22 °C, 35 °C and 38 °C.

Effects of ectoine on behavioral, physiological and biochemical parameters of Daphnia magna exposed to dimethyl sulfoxide.

DMSO is a very common solvent for hydrophobic chemicals that may pose a threat to aquatic organisms. Ectoine (ECT) is a protective amino acid produced by various strains of halophilic bacteria with high potential to alleviate detrimental effects induced by environmental stressors. This amino acid is used in many cosmetics and pharmaceuticals may enter aquatic ecosystems interacting with ions and macromolecules.

Procaine penicillin alters swimming behaviour and physiological parameters of Daphnia magna.

Procaine penicillin (PP) is a β-lactam antibiotic widely used in human and veterinary medicine. Although PP is detected in surface water, little is known on its effects on aquatic invertebrates. Our aim was to determine the influence of PP on swimming behaviour (track density, swimming speed, turning angle, hopping frequency) and physiological activity (oxygen consumption, heart rate, thoracic limb movement) of a freshwater invertebrate Daphnia magna exposed to PP at concentrations of 11.