Output-Based Decentralized Adaptive Event-Triggered Control of Interconnected Systems With Sensor/Actuator Failures.

This article presents a double-channel (sensor-to-controller channel and controller-to-actuator channel) event triggered control method for nonlinear interconnected systems subject to sensor and actuator faults via the backstepping technique. It should be emphasized that the utilization of triggering mechanism at the sensor side poses a challenge to the design of backstepping control, as it leads to nondifferentiable virtual control signals due to the discontinuous nature of the state/output signals received at the controller side. In contrast to existing methods, the proposed event triggering mechanism eliminates the need for computing virtual control signals at the sensor side before transmitting them to the controller side.

Fruit wines classification enabled by combing machine learning with comprehensive volatiles profiles of GC-TOF/MS and GC-IMS.

Fruit wines, produced through the fermentation of various fruits, are well-documented for their distinct flavor profiles. Intelligent sensory analysis, GC-TOF/MS and GC-IMS were used for the analysis of the volatile profile of eight types of fruit wines including 5 grape wine (SJ, LS, HY, TJ, FT), 1 fermented plum wine (FZ), 1 blueberry wine (HZ), as well as 1 configured plum wine (LM). A total of 281 compounds were identified through GC-TOF/MS, with esters and acids constituting over 80% of all samples.

Tailored Lead-Free KNN/BFO Perovskite Ferroelectric Film Heterostructures for Enhanced Electrofunctional Properties via Interface-Modulated Bilayer Design.

In this work, a bilayer lead-free perovskite ferroelectric structure was fabricated comprising a highly polar BiFeO (BFO) bottom layer and a less polar (KNa)NbO (KNN) top layer. The BFO sublayer, deposited via radio frequency magnetron sputtering without postgrowth annealing, not only exhibited enhanced crystallinity but also promoted superior microstructural properties in the sol-gel derived KNN overlayer, thereby ensuring excellent intrinsic electrical properties. Compared to the poorly crystallized single-layer KNN films directly synthesized on LNO-buffered (100)-Si substrate, the KNN layer in the bilayer structure demonstrated a strong (100) texture, along with a dense, homogeneous, fine-grained morphology.

A high-efficient protoplast transient system for screening gene editing elements in Salvia miltiorrhiza.

A high-efficiency protoplast transient system was devised to screen genome editing elements in Salvia miltiorrhiza. Medicinal plants with high-value pharmaceutical ingredients have attracted research attention due to their beneficial effects on human health. Cell wall-free protoplasts of plants can be used to evaluate the efficiency of genome editing mutagenesis.

Initial IL-10 production dominates the therapy of mesenchymal stem cell scaffold in spinal cord injury.

Spinal cord injury (SCI) is an acute damage to the central nervous system that results in severe morbidity and permanent disability. Locally implanted scaffold systems with immobilized mesenchymal stem cells (MSCs) have been widely proven to promote locomotor function recovery in SCI rats; however, the underlying mechanism remains elusive. In this study, we constructed a hyaluronic acid scaffold system (HA-MSC) to accelerate the adhesive growth of human MSCs and prolong their survival time in SCI rat lesions.

Application of serum Raman spectroscopy combined with classification model for rapid breast cancer screening.

Introduction: This study aimed to evaluate the feasibility of using general Raman spectroscopy as a method to screen for breast cancer. The objective was to develop a machine learning model that utilizes Raman spectroscopy to detect serum samples from breast cancer patients, benign cases, and healthy subjects, with puncture biopsy as the gold standard for comparison. The goal was to explore the value of Raman spectroscopy in the differential diagnosis of breast cancer, benign lesions, and healthy individuals.

Research of integrated shimming Halbach magnet for High strength, compact Benchtop NMR device.

Due to production and assembly errors of magnets resulting in reduced magnetic field homogeneity, passive shimming (PS) is necessary when Halbach magnets are used in high-resolution Benchtop Nuclear Magnetic Resonance (BNMR) spectrometer. The conventional PS technique, which places independent PS devices inside the magnet aperture, is no longer applicable to small-aperture compact high-field-strength Halbach magnet studies. In this paper, based on spherical harmonic function expansion, we improve the magnetic field homogeneity by optimizing the moving step of the magnet moving arrays composed of Halbach magnets to generate the corresponding harmonic terms to compensate for the magnetic field.

The Light- and Jasmonic Acid-Induced Positive Regulates the Initiation of Glandular Secretory Trichome in L.

The plant L. is famous for producing "artemisinin", which is an essential component in the treatment of malaria. The glandular secretory trichomes (GSTs) on the leaves of secrete and store artemisinin.

Piriformospora indica alter root-associated microbiome structure to enhance Artemisia annua L. tolerance to arsenic.

Microorganisms in the rhizosphere are crucial allies for plant stress tolerance. Recent research suggests that by interacting with the rhizosphere microbiome, microorganisms can aid in the revegetation of soils contaminated with heavy metal(loid)s (HMs). However, it is unknown that how Piriformospora indica influences the rhizosphere microbiome to mitigate arsenic-toxicity in arsenic-enriched environments.

JA-Regulated AaGSW1-AaYABBY5/AaWRKY9 Complex Regulates Artemisinin Biosynthesis in Artemisia annua.

Artemisinin, a sesquiterpene lactone obtained from Artemisia annua, is an essential therapeutic against malaria. YABBY family transcription factor AaYABBY5 is an activator of AaCYP71AV1 (cytochrome P450-dependent hydroxylase) and AaDBR2 (double-bond reductase 2); however, the protein-protein interactions of AaYABBY5, as well as the mechanism of its regulation, have not yet been elucidated. AaWRKY9 protein is a positive regulator of artemisinin biosynthesis that activates AaGSW1 (glandular trichome-specific WRKY1) and AaDBR2 (double-bond reductase 2).

AaSEPALLATA1 integrates jasmonate and light-regulated glandular secretory trichome initiation in Artemisia annua.

Glandular secretory trichomes (GSTs) can secrete and store a variety of specific metabolites. By increasing GST density, valuable metabolites can be enhanced in terms of productivity. However, the comprehensive and detailed regulatory network of GST initiation still needs further investigation.

AaWIN1, an AP2/ERF protein, positively regulates glandular secretory trichome initiation in Artemisia annua.

Exploring the genetic network of glandular trichomes and manipulating genes relevant to secondary metabolite biosynthesis are of great importance and value. Artemisinin, a key antimalarial drug ingredient, is synthesized and stored in glandular secretory trichomes (GSTs) in Artemisia annua. WIN/SHN proteins, a clade of AP2/ERF family, are known as regulators for cuticle biosynthesis.

Theoretical foundation for designing multilayer Halbach array magnets for benchtop NMR and MRI.

Multilayer Halbach array magnets support portable NMR and MRI, but optimizing their design to maximize performance and minimize the use of expensive magnet materials is challenging. This is partly because our theoretical understanding of such arrays is incomplete and computationally intensive. Here we provide a theoretical description of the magnetic field distribution and we demonstrate that inhomogeneity is greatest along the z axis in multilayer Halbach array magnets.

Engineering the expression of plant secondary metabolites-genistein and scutellarin through an efficient transient production platform in L.

Plant natural products (PNPs) are active substances indispensable to human health with a wide range of medical and commercial applications. However, excessive population growth, overexploitation of natural resources, and expensive total chemical synthesis have led to recurrent supply shortages. Despite the fact that the microbial production platform solved these challenges, the platform still has drawbacks such as environmental pollution, high costs, and non-green production.

MADS-box gene promotes artemisinin biosynthesis in .

The plant is well known for its production of artemisinin, a sesquiterpene lactone that is an effective antimalarial compound. Although remarkable progress has been made toward understanding artemisinin biosynthesis, the effect of MADS-box family transcription factors on artemisinin biosynthesis is still poorly understood. In this study, we identified a MADS transcription factor, AaSEP4, that was predominantly expressed in trichome.

A high-efficiency trichome collection system by laser capture microdissection.

Trichomes, which are classified as glandular or non-glandular, are hair-like epidermal structures that are present on aerial parts of most plant species. Glandular secretory trichomes (GSTs) have the capacity to secrete and store specialized metabolites, which are widely used as natural pesticides, food additives, fragrance ingredients or pharmaceuticals. Isolating individual trichomes is an essential way for identifying trichome-specific gene functions and discovering novel metabolites.

Application of Surface-Enhanced Raman Spectroscopy in the Screening of Pulmonary Adenocarcinoma Nodules.

This study is aimed at evaluating the feasibility of a screening method for the pulmonary adenocarcinoma nodules through surface-enhanced Raman spectroscopy (SERS). . Using SERS to measure serum from pulmonary nodules and healthy subjects, intraoperative biopsy pathological diagnosis was regarded as the gold standard for labeling serum samples.

The truncated AaActin1 promoter is a candidate tool for metabolic engineering of artemisinin biosynthesis in Artemisia annua L.

Malaria is a devastating parasitic disease with high levels of morbidity and mortality worldwide. Artemisinin, the active substance against malaria, is a sesquiterpenoid produced by Artemisia annua. To improve artemisinin content in the native A.

A passive shimming method for Halbach magnet based on magnetic sheet arrays.

Halbach magnet has great potential in nuclear magnetic resonance device, where the field homogeneity requirement puts heavy demands on high efficiencypassive shimming (PS) technique. Conventional PS involves a tedious iteration process of the magnetic block/sheet number and positions optimization. In this paper, we propose a PS method based on magnetic sheet arrays (MSAs) targeting at spherical harmonic basis up to the 3rd order including dedicated composition of Y(4Z2-X2-Y2), Z3 and X(4Z2-X2-Y2) (n = 3, m = -1,0,1) with cross terms to implement structural field compensation.

Switching-behavior improvement in HfO/ZnO bilayer memory devices by tailoring of interfacial and microstructural characteristics.

We investigated the effect of top contact interface and microstructural characteristics of the insulating layers on resistive switching behaviors by fabricating and characterizing the HfO/ZnO bilayer heterostructures. Different thickness of ZnO underlying layer and different deposition temperatures of the upper HfOlayer were designed to analyze the intrinsic contribution of the crystalline microstructure of the insulating bilayer. Pt and Ti top electrodes were used to demonstrate the extrinsic contribution of the interface configuration.

A high-efficiency Agrobacterium-mediated transient expression system in the leaves of Artemisia annua L.

Background: The Agrobacterium-mediated transient transformation, which proved effective in diverse plant species, has been widely applied for high-throughput gene function studies due to its simplicity, rapidity, and high efficiency. Despite the efforts have made on Artemisia annua transient expression, achieving high-throughput gene functional characterization basing on a fast and easy-manipulated transient transformation system in A. annua remains challenging.

AaWRKY17, a positive regulator of artemisinin biosynthesis, is involved in resistance to Pseudomonas syringae in Artemisia annua.

Artemisia annua, a traditional Chinese medicinal plant, remains the only plant source for artemisinin production, yet few genes have been identified to be involved in both the response to biotic stresses, such as pathogens, and artemisinin biosynthesis. Here, we isolated and identified the WRKY transcription factor (TF) AaWRKY17, which could significantly increase the artemisinin content and resistance to Pseudomonas syringae in A. annua.

AaWRKY9 contributes to light- and jasmonate-mediated to regulate the biosynthesis of artemisinin in Artemisia annua.

Artemisinin, isolated from Artemisia annua, is recommended as the preferred drug to fight malaria. Previous research showed that jasmonate (JA)-mediated promotion of artemisinin accumulation depended on light. However, the mechanism underlying the interaction of light and JA in regulating artemisinin accumulation is still unknown.

Transcriptomic analysis reveals the parallel transcriptional regulation of UV-B-induced artemisinin and flavonoid accumulation in Artemisia annua L.

UV-B radiation is a pivotal photomorphogenic signal and positively regulates plant growth and metabolite biosynthesis. In order to elucidate the transcriptional regulation mechanism underlying UV-B-induced artemisinin and flavonoid biosynthesis in Artemisia annua, the transcriptional responses of A. annua L.