Safety evaluation of a whey protein fraction containing a concentrated amount of naturally occurring TGF-β2.

TM0601p is a whey protein isolate derived from cow milk, containing a concentrated amount of transforming growth factor β2 (TGF-β2), and is intended for nutritional use in infants and adults. In vivo and in vitro studies have been performed to evaluate the safety of this product. In a 13-week toxicity study, treatment of adult Sprague-Dawley rats by gavage at up to 2000mg/kg/day did not result in any significant findings other than minor non-adverse changes in urinary parameters in females.

Effects of simultaneous active immunization against 17 beta-estradiol and testosterone on pituitary and ovarian activity in the rat.

Female rats were immunized with mixed 17 beta-estradiol-6-carboxymethyloxime-bovine serum albumin and testosterone-3-carboxymethyloxime-bovine serum albumin. All the animals produced antibodies against the 2 haptens and were better immunized against 17 beta-estradiol than against testosterone. Nevertheless the disappearance of cyclic ovarian function was only obtained in animals highly immunized against the two antigens.

[Effect of proteins from bovine milk serum on the multiplication of human cancerous cells].

The addition of bovine milk whey to the culture medium of human cancerous cells (MCF-7 and PC-3) results in a significant reduction of cells growth. Milk whey acts more efficiently on MCF-7 than on PC-3 growth. The inhibition could be due to a protein.

Stimulation by estradiol-17 beta of thymidine kinase activity in the rat uterus.

The action of estradiol-17 beta (E2) on thymidine kinase (TK) activity was studied in uteri from immature female rats. It was demonstrated that a single injection of E2 highly stimulated the enzyme activity which reached its maximum level 24 h after hormone administration. Physiological amounts of E2 were efficient and changes in TK activity were observed exclusively in uterus and liver.

[Inhibitory effects of Celiptium on thymidine kinase synthesis induced in the uterus by 17 beta-estradiol].

Inhibitory effects of Celiptium on the thymidine kinase synthesis induced by oestradiol-17 beta in the rat uterus. In the rat uterus, the synthesis of thymidine kinase specifically induced by oestradiol-17 beta was inhibited by Celiptium. The synthesis was totally inhibited when the drug was administered before the oestrogen and partially when it was administered after.

Active immunization of female rats against 17 beta-estradiol. Preliminary studies on 17 beta-estradiol binding in uterine and pituitary cytosols.

Female rats were immunized with 17 beta-estradiol-6-carboxymethyloxime-bovine serum albumin. They developed antibodies to estradiol and, to a very low extent, antibodies to BSA. Anti-estradiol antibodies possessed tight specificity to estradiol-17 beta, without cross-reactivities with other estrogens.

[Induction, by 17 beta-estradiol, of thymidine kinase activity in uteri of rats].

In uteri from adult female rats, Thymidine Kinase activity varied during ovarian cycle and was maximum at metestrus. Subcutaneous injections of 5, 10 or 25 ng of estradiol-17 beta to immature female rats, resulted in a 3, 5 or 10 fold increase of enzyme activity. Moreover, Thymidine Kinase activity was decreased by injection of medroxyprogesterone alone or associated with estradiol-17 beta.

[Effect of active immunization against 17 beta-estradiol on cytosolic estrogen receptors in the rat uterus].

Following active immunization of female rats against estradiol-17 beta, the amount of specific binding sites for estrogen decreased in uterine cytosol as a function of antiserum titres. They were undetected when antibodies titres were higher than 1/2000. Moreover, a binding protein specific for estradiol-17 beta appeared.