Study of interaction in dual-species biofilm of Candida glabrata and Klebsiella pneumoniae co-isolated from peripheral venous catheter using Raman characterization mapping and machine learning algorithms.

Polymicrobial biofilm infections, especially associated with medical devices such as peripheral venous catheters, are challenging in clinical settings for treatment and management. In this study, we examined the mixed biofilm formed by Candida glabrata and Klebsiella pneumoniae, which were co-isolated from the same peripheral venous catheter. Our results revealed that C.

In vitro activity of amphotericin B in combination with imipenem/colistin against mixed cultures of Candida spp. and Gram-negative bacteria.

A new direction of research was established due to biofilms' infections caused by a mixture of fungal and bacterial species. Diagnosis of these infections becomes more difficult and high doses of drugs are used in treatment, especially in critically ill patients. The aim of the current study was to examine the effects of amphotericin B, in combination with imipenem or colistin against Candida albicans - Acinetobacter baumannii- Proteus mirabilis and Candida tropicalis - Acinetobacter baumannii -Proteus mirabilis polymicrobial biofilms.

Antioxidant, anti-inflammatory, antiulcerogenic, and haemolytic properties of hydroethanolic and aqueous extracts from Benth. fruit.

Anti-inflammatory, antioxidant, antiulcer, and cytotoxic properties of hydroethanolic and aqueous extracts from the fruits of Tabernaemontana crassa were investigated. Four phenolic compounds, including three phenolic acids (gallic, caffeic, and chlorogenic acids) and one flavonol (rutin), were detected by HPLC-PDA. The hydroethanol extract (HE) exhibited high antioxidant activity and inhibition of haemolysis against red blood cells.

Exploring the dynamics of mixed-species biofilms involving Candida spp. and bacteria in cystic fibrosis.

Cystic fibrosis (CF) is an inherited disease that results from mutations in the gene responsible for the cystic fibrosis transmembrane conductance regulator (CFTR). The airways become clogged with thick, viscous mucus that traps microbes in respiratory tracts, facilitating colonization, inflammation and infection. CF is recognized as a biofilm-associated disease, it is commonly polymicrobial and can develop in biofilms.

Evaluation of mixed biofilm production by Candida spp. and Staphylococcus aureus strains co-isolated from cystic fibrosis patients in northwest Algeria.

Cystic fibrosis patients' lungs are chronically colonized by multiple microbial species capable of forming biofilms. This study aimed to characterize the polymicrobial biofilm formed by Candida spp. and S.

Initial characteristics of cystic fibrosis in Algeria: Description of 34 pediatric cases.

Background: Cystic fibrosis (CF) is a rare disease in Algeria, and its prognosis is poor in developing countries. The clinical and demographic knowledge of Algerian pediatric patients diagnosed with CF is incomplete due to the nonexistence of a national medical registry. Hence, the present study is the first Algerian multicentre study on CF.

In vitro evaluation of biofilm formation by Candida parapsilosis and Enterobacter cloacae. Scanning electron microscopy and efficacy of antimicrobial combinations study.

Fungal-bacterial infections are being increasingly recognized in clinical settings, and the interaction between these species in polymicrobial biofilms often lead to infections that are highly resistant to treatment. In this in vitro study, we analyzed the formation of mixed biofilms using clinically isolated Candida parapsilosis and Enterobacter cloacae. Additionally, we assessed the potential of conventional antimicrobials, both alone and in combination, for treating polymicrobial biofilms built by these human pathogens.

Influence of ascorbic acid and α-tocopherol on the autoxidation and in vitro antifungal activity of amphotericin B.

Background And Purpose: Amphotericin B (AmB) is the standard treatment for systemic fungal infections; however, the formation of reactive oxygen species reduces the efficacy and stability of this molecule. The present study aimed to evaluate the effect of the combination of AmB with ascorbic acid and α-tocopherol on its autoxidation and antifungal activity.

Materials And Methods: The antifungal activity against was evaluated by the viable cell counting method and checking their morphological changes with a scanning electron microscope.

Interaction in a dual-species biofilm of Candida albicans and Candida glabrata co-isolated from intravascular catheter.

The use of catheters for vascular access may be associated with colonization by Candida species and their biofilm-forming ability. The latter can harbor two or more species of Candida yeast. In the sense, we conducted our study at the University Hospital of Tlemcen in west Algeria at the neuro-surgery unit, that aims (or which aims) to evaluate the ability to form mixed biofilm by dual-species Candida albicans/Candida glabrata co-isolated from intravascular catheters and their interaction in biofilm.

Erratum: Touil, H.F.Z. et al. Optimum Inhibition of Amphotericin-B-Resistant Strain in Single- and Mixed-Species Biofilms by and Non- Terpenoids. Biomolecules 2020, 10, 342.

The authors wish to make the following correction to this paper [1] [...

Optimum Inhibition of Amphotericin-B-Resistant Strain in Single- and Mixed-Species Biofilms by and Non- Terpenoids.

is one of the most common human fungal pathogens and represents the most important cause of opportunistic mycoses worldwide. Surgical devices including catheters are easily contaminated with via its formation of drug-resistant biofilms. In this study, amphotericin-B-resistant strains were isolated from surgical devices at an intensive care center.

Effects of tyrosol and farnesol on Candida albicans biofilm.

The present in vitro study examined the effects of the quorum‑sensing molecules farnesol and tyrosol on the development of Candida albicans biofilm in order to elucidate their role as novel adjuvants in oral hygiene. The investigation was conducted in C. albicans ATCC 10231 and C.

Ex vivo decontamination of yeast-colonized dentures by iodine-thiocyanate complexes.

Introduction: Under well-defined experimental conditions, and in the presence of hydrogen peroxide, lactoperoxidase produces stable iodine-thiocyanate complexes that have antimicrobial properties. A novel process was developed to short circuit the consumption of hydrogen peroxide by microbial catalases by producing iodine-thiocyanate complexes prior to contact with microorganisms, with the aim of being able to decontaminate the ex vivo dentures colonized by yeasts.

Materials And Methods: Teabags containing lactoperoxidase adsorbed on inert clay beads were immersed for 1 minute in phosphate buffer solution (0.

In vitro activity of antifungal combinations against planktonic and sessile cells of Candida albicans isolated from medical devices in an intensive care department.

Background: Invasive fungal infections are an emerging health problem worldwide. They are responsible for a significant rate of morbidity and mortality. Infections caused by Candida albicans involve proliferation of biofilms on biotic or abiotic surface.

Cosmetics Preservation: A Review on Present Strategies.

Cosmetics, like any product containing water and organic/inorganic compounds, require preservation against microbial contamination to guarantee consumer’s safety and to increase their shelf-life. The microbiological safety has as main goal of consumer protection against potentially pathogenic microorganisms, together with the product’s preservation resulting from biological and physicochemical deterioration. This is ensured by chemical, physical, or physicochemical strategies.

Risk assessment for the spread of Candida sp. in dental chair unit waterlines using molecular techniques.

Aims: The aim of this study was to evaluate the presence of yeasts in dental chair unit waterlines (DCUWLs) and to test their ability to form biofilms.

Materials And Methods: Eighteen dental waterlines were analysed by culture in liquid Sabouraud in order to allow the quantification and the purification of isolated yeasts from their internal surfaces. All isolates were identified by standard laboratory procedures, including CHROMagar Candida medium for orientation, commercial yeast identification system Api Candida, MALDI-TOF MS and DNA sequencing.

[Study of the infectivity of Candida on peripheral vascular catheters collected from the University Hospital of Tlemcen].

Yeasts can adhere to medical implants and cause infections responsible for high morbidity and mortality among hospitalized patients. The objective of this study is to investigate the infectivity on peripheral vascular catheters collected from general surgery and cardiology in University Hospital of Tlemcen. The results showed that from 29 samples altered by yeast, 35 Candida sp.

Retrospective study of Candida sp. contaminations of endoscopes at the University Hospital of Tlemcen (Algeria).

Background: Improper cleaning and disinfection of endoscopes has been responsible for multiple nosocomial outbreaks and sometimes serious life-threatening infections.

Objective: The aim of our study is, at first, to identify Candida species responsible for the contamination of endoscopes, and to determine the minimal inhibitory concentrations of planktonic (MIC) and sessile cells (SMIC) of amphotericin B (AmB) against our isolated strains.

Materials And Methods: The present study was performed on four endoscopes in the department of gastroenterology at the University Hospital of Tlemcen (Algeria).

Dose‑dependent effect of lysozyme upon Candida albicans biofilm.

The present study investigated the in vitro effect of lysozyme (0-1,000 µg/ml) on Candida albicans (C. albicans) biofilm development. Investigations were conducted on C.

[Infectious risk related to the formation of multi-species biofilms (Candida - bacteria) on peripheral vascular catheters].

The Candida yeasts are the fourth leading cause of death from systemic infections, the risk may increase when the infection also involves bacteria. Yeasts and bacteria can adhere to medical implants, such as peripheral vascular catheters, and form a multicellular structures called "mixed biofilms" more resistant to antimicrobials agents. However, the formation of mixed biofilms on implants leads to long-term persistent infections because they can act as reservoirs of pathogens that have poorly understood interactions.

Study of strains of Candida spp. Isolated from catheters in UHC of Oran (Algeria): Identification and antifungal susceptibility.

Objectives: The increasing incidence of Candida spp., and the vital prognosis often compromise for patients with Candida species make urgent the exact knowledge of their distribution worldwide and exhaust action antifungals currently used in clinical. That why we carry out an epidemiological study of Candida species and testing their susceptibility against two antifungals: amphotericin B and caspofungin.

[Evaluation of mixed biofilm formation between Candida albicans and a variety of bacterial species isolated from peripheral catheters at Tlemcen CHU. First study in Algeria].

Introduction: Mixed-species biofilms constitute a reservoir of infection for a group of bacteria and yeasts that coexist on the same support. Peripheral venous catheters make up a good surface for the attachment of microorganisms that promote biofilm formation and this requires complex strategies for antimicrobial treatments.

Objective: No such studies on formation mixed biofilms have ever been conducted in Algeria.

[Fungal infectivities of implanted catheters due to Candida sp. Biofilms formation and resistance].

Background: Candidemia are the most common fungal infections in hospitals. However, the catheters are subject to be altered by Candida biofilms which increase the risk of invasive nosocomial infections due to the high resistance to antifungal agents. Therefore, the minimum inhibitory concentrations of planktonic (MIC) and sessile cells (CIMS) were evaluated.

In vitro susceptibility of amphotericin-B, voriconazole and caspofungin against Candida guilliermondii biofilms, isolated from dentals units water pipes, under different growth phases.

Objective: The dental units water pipes are a favorable medium for biofilms formation because of the small diameter of the pipe and the duration of water stagnation, but the question which arises is the nature of the biofilms which are formed inside? This article gives a progress report on the nature of this microbial contamination and precisely the fungal biofilms formation by examining their susceptibility to antifungal agents under different growth phases.

Methods: Sixteen samples of dental units water pipes were taken from public dental clinic and from stomatology unit at the university hospital of Tlemcen (Algeria). The isolated strains were identified by the conventional mycological methods and were analyzed to determine their minimal concentrations inhibiting their growth (planktonic and sessile forms) using three antifungal agents.