Publications by authors named "Boualam Mahmoud Abdelwadoud"
Article Synopsis
- The methanogenic archaea have been part of human oral microbiota since the Palaeolithic era and became more prevalent from the 18th century onward.
- Initial isolation of this organism was achieved from dental plaque of healthy individuals, leading to studies focused on improving its laboratory culture methods.
- Current identification techniques, including a specialized RTQ-PCR system, along with next-generation sequencing, have revealed its presence in various oral and extra-oral sites, highlighting its potential role in health and disease.
Article Synopsis
- Researchers found it hard to study old malaria cases in Europe because there wasn't enough preserved material, but they discovered that dental pulp (inside teeth) can help detect ancient parasites.
- They collected teeth from 23 people buried between the 9th and 13th centuries in Corsica and used advanced tests to find DNA and proteins.
- The tests showed that nine teeth had signs of malaria parasites, supporting what was known from history and giving more info about how malaria affected people in the Mediterranean a long time ago.
The oral fluid microbiome comprises an important bacterial diversity, yet the presence of archaea has not been reported so far. In order to quest for the presence of methanogenic archaea (methanogens) in oral fluid, we used a polyphasic approach including PCR-sequencing detection, microscopic observation by fluorescence in-situ hybridization, isolation and culture, molecular identification and genotyping of methanogens in 200 oral fluid specimens. In the presence of negative controls, 64/200 (32%) prospectively analysed oral fluid specimens were PCR-positive for methanogens, all identified as Methanobrevibacter oralis by sequencing.
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