Publications by authors named "Bosilkovski Mile"
Immune evasion strategies of Brucella, the etiologic agent of brucellosis, a global zoonosis, remain partially understood. The omentum, a tertiary lymphoid organ part of visceral adipose tissue, has never been explored as a Brucella reservoir. We report that B.
View Article and Find Full Text PDFBackground: Brucellosis is a zoonotic infection that presents a major challenge to public health worldwide.
Aim: This research compares demographic characteristics, clinical features, and outcomes across different age groups in patients with human brucellosis.
Material And Methods: Between 2003 and 2022 a total of 508 patients with brucellosis were subjected to diagnostics and treatment at the Clinic for Infectious Diseases in Skopje.
Objectives: The Balkan Peninsula, acting as a crossroad between central Europe and the Middle East, presents diverse ecosystems supporting various tick species capable of transmitting TBDs. This study focuses on Serbia and North Macedonia, both endemic for TBDs, aiming to investigate human-biting ticks' prevalence, TBD prevalence, and major TBPs in blood samples.
Patients And Methods: This prospective observational study was conducted in 2022 at two medical centers, involving 45 patients from Novi Sad, Serbia, and 17 patients from Skopje, North Macedonia.
Background: Maternal, fetal, and neonatal complications of brucellosis in pregnant women are probably higher than those in the general population. This comparative study aimed to survey the mentioned complications in pregnant women with positive and negative serologic tests.
Study Design: This is a prospective cohort study.
Lyme borreliosis (LB) and tick-borne encephalitis (TBE) are important tick-borne diseases in Europe. This study aimed to investigate the seroreactivity against and TBE virus (TBEV) in tick-infested individuals in North Macedonia and Serbia. Serum samples were collected from tick-infested individuals and from healthy individuals in the same regions.
View Article and Find Full Text PDFArticle Synopsis
- Bacteria from the genus Brucella cause brucellosis, a serious disease affecting both animals and humans, and have been controversially merged with other unrelated bacterial species based on genomic findings.
- Researchers argue this merger is inappropriate due to lack of thorough phylogenetic analysis and exclusion of expert opinions in brucellosis.
- They warn that combining these groups could lead to confusion and risks in public health, particularly impacting those dealing with brucellosis in under-resourced regions, and call for keeping the Brucella genus distinct.
Article Synopsis
- Hematopoietic stem cells (HSC) are primarily known for maturing into immune cells that respond to infections, but recent findings indicate they can also directly detect cytokines during infections and maintain memory of them.
- HSC's direct sensing of danger signals leads to early myeloid commitment, enhancing the production of myeloid cells that are crucial for a robust immune response.
- The study reveals that HSC can recognize the pathogenic bacteria B. abortus in the bone marrow through the interaction of the CD150 protein with a bacterial protein (Omp25), marking the first time this direct pathogen recognition by HSC has been observed.
Background: Plasmodium falciparum is the leading cause of imported malaria and the most common cause of death in returning travellers.
Aim: To identify the main epidemiological and clinical characteristics of patients with imported falciparum malaria in the Republic of North Macedonia.
Material And Methods: Retrospectively analyzed were the epidemiological and clinical features of 34 patients with imported falciparum malaria who were diagnosed and treated at the university clinic for infectious diseases and febrile conditions in Skopje from 2010 to 2022.
Article Synopsis
- The study focuses on the prevalence of antibiotic resistance in Brucella melitensis strains isolated from patients in Bosnia and Herzegovina, highlighting significant human health concerns due to brucellosis.
- Among the 108 strains analyzed, a notable 84.3% showed resistance to trimethoprim-sulfamethoxazole in standard broth, while over 90% were resistant to azithromycin in multiple testing environments.
- The findings suggest that alternative testing methods, like enriched broth, may improve understanding of antimicrobial resistance, indicating a need for further genomic studies to investigate the underlying mechanisms of resistance in these bacteria.
[Not Available].
Sante Publique
September 2021
Introduction: Brucellosis, the most common zoonosis globally, is considered a major public health problem.
Objective: A retrospective study was carried out over 21 years (1998-2018) to determine the epidemiological features of human brucellosis in the province of El-Oued (south-eastern Algeria).
Results: 1,832 confirmed cases of human brucellosis were reported during the study period, with an average incidence rate of around 12.
The aim of this study was to evaluate the ability of severity scoring systems to predict 30-day mortality in patients with severe community-acquired pneumonia. The study included 98 patients aged ≥18 years with community acquired pneumonia hospitalized at the Intensive Care Unit of the University Clinic for Infectious Diseases in Skopje, Republic of North Macedonia, during a 3-year period. We recorded demographic, clinical and common biochemical parameters.
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- Prompt and adequate treatment is crucial for managing human brucellosis since eradication of the animal source and effective human vaccines are currently unavailable.
- The goal of treatment is to reduce symptoms, complications, relapses, and chronic cases, but relapses are common due to the bacteria's ability to survive inside host cells.
- Future treatment strategies may include new antimicrobial agents, immunomodulation, and targeting specific molecular bacterial functions to enhance effectiveness.
Cat Scratch Disease: The First Case Report in Republic of North Macedonia.
Pril (Makedon Akad Nauk Umet Odd Med Nauki)
December 2020
Article Synopsis
- Cat scratch disease (CSD), caused by the Bartonella henselae bacterium, primarily affects immuno-competent individuals and is transmitted from cats through scratches or bites.
- A case report details the first confirmed incidence of CSD in a specific country, involving a healthy adult female who experienced fever and lymphadenopathy over a month, ultimately diagnosed via serological testing.
- Treatment with azithromycin led to her complete recovery, underscoring the need for awareness among healthcare professionals to consider CSD in patients with unexplained fever and lymphadenopathy, particularly those with possible cat exposure.
Prevalence of Rotaviruses in the Etiology of Acute Diarrhea in Young Children, Clinical forms, Extraintestinal Manifestations and Complications.
Pril (Makedon Akad Nauk Umet Odd Med Nauki)
December 2020
Rotavirus is highly contagious factor with dominant feces-oral transmission. Because it is stable in external environment, transmission clusters are possible by close contact, ingestion of contaminated water or food or contact with contaminated surfaces. It survives within hours and days on hands and contaminated surfaces.
View Article and Find Full Text PDFDifferent outcomes of brucellosis in pregnancy regarding the fetus/neonate and the mother are described. Medical records of five pregnant women with brucellosis were retrospectively analyzed. Patients were treated in several departments of infectious diseases in the Republic of Macedonia between 1995 and 2009.
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- Brucellosis is prevalent in Iran, especially in western regions like Hamadan province, and this study focused on its seroprevalence and risk factors in the Famenin population.
- A total of 2,367 participants were surveyed, yielding a seroprevalence of 6.6% based on the Wright test, with significant links to occupational exposure and contact with livestock.
- The findings indicate a notable public health concern, highlighting occupations related to animal husbandry and farming as key risk factors for brucellosis infection.
Article Synopsis
- Human brucellosis during pregnancy leads to more adverse outcomes compared to healthy pregnancies, but these outcomes are less severe than those observed in animals.
- The prevalence of brucellosis in pregnant women in endemic areas ranges from 1.5% to 16.9%, with serious complications like abortions (2.5-54.5%) and preterm deliveries (1.2-28.6%).
- Prompt treatment with a combination of rifampicin and trimethoprim-sulfamethoxazole is crucial to mitigate risks, and preventive measures like screening and education for women of childbearing age are essential in affected regions.
Acute Thyroiditis Associated with Brucellosis: A Case Report.
Pril (Makedon Akad Nauk Umet Odd Med Nauki)
October 2019
Brucella thyroiditis represents an extremely rare focal form of brucellosis. In this case report we describe a 55 years old male, diagnosed with brucellosis and peripheral arthritis with subsequent development of acute thyroiditis. The symptoms duration consistent with brucellosis started two weeks before establishing the diagnosis.
View Article and Find Full Text PDFCharacteristics of an Outpatient Cohort with HBeAg-Negative Chronic Hepatitis B.
Open Access Maced J Med Sci
July 2019
Background: Patients with hepatitis Be antigen-negative chronic hepatitis B (HBeAg-negative CHB), and patients' inactive carriers (IC) have similar laboratory and serologic characteristics and are not always easy to distinguish.
Aim: To characterise hepatitis Be antigen (HBeAg) negative chronic hepatitis B cohort based on their laboratory and virology evaluations at one point of time.
Methods: A prospective non-randomized study was conducted on 109 patients with HBeAg negative chronic hepatitis B treated as outpatients at the Clinic for Infectious Diseases and Febrile Conditions.
Our study assesses the influence of illness duration before establishing the diagnosis of brucellosis and initiating therapy on patients' main clinical characteristics and outcome in an endemic area. The medical files of 297 patients with brucellosis were retrospectively analysed. They were divided into four groups according to illness duration before initiating therapy: <10 days; 11-30 days; 31-90 days; and >90 days.
View Article and Find Full Text PDFBrucellosis in pregnancy: results of multicenter ID-IRI study.
Eur J Clin Microbiol Infect Dis
July 2019
Article Synopsis
- The study focuses on the impact of brucellosis in pregnant women, highlighting that it is linked to various obstetric complications, yet there is limited data on human cases.
- Data was gathered from 242 pregnant women diagnosed with brucellosis across 23 hospitals over a 14-year period, revealing that 14.0% experienced obstetric complications, with spontaneous abortion being the most common at 6.1%.
- Key factors related to obstetric complications included symptoms like splenomegaly, nausea, and anemia, emphasizing the need for investigation in pregnant women who show such signs, especially in endemic areas.
Introduction: The study aimed to compare the etiologic spectrum of diseases causing fever of unknown origin (FUO) and methods for definitive diagnosis in a tertiary care hospital in the Republic of North Macedonia during two different time periods.
Patients And Methods: There were analysed retrospectively the causes for FUO and final diagnostic approaches in 185 patients with classic FUO that were treated at the University Hospital for Infectious Diseases in Skopje during two time periods. Seventy nine patients were treated during 1991 to 1995 and 106 patients during 2011 to 2015.
Background: Liver biopsy for evaluation of liver fibrosis has several adverse effects, for which reason noninvasive tests have been developed.
Aim: To evaluate the usefulness of noninvasive biomarkers, qHBsAg and HBV DNA levels in predicting liver fibrosis in patients with hepatitis Be antigen (HBeAg) negative chronic hepatitis B (CHB).
Material And Methods: This prospective study included 50 patients with HBeAg negative CHB.