Introducción: El enojo es frecuente en los trastornos emocionales y se asocia a mayor severidad y peor respuesta al tratamiento. Las intervenciones realizadas desde la terapia dialéctico comportamental (DBT) pueden ser eficaces para lograr un manejo efectivo del enojo. El objetivo del trabajo fue determinar la efectividad de participar de un grupo de entrenamiento en habilidades para el manejo efectivo del enojo.
View Article and Find Full Text PDFChitinases are promising enzymes for a multitude of applications, including chitooligosaccharide (COS) synthesis for food and pharmaceutical uses and marine waste management. Owing to fungal diversity, fungal chitinases may offer alternatives for chitin degradation and industrial applications. The rapid reproduction cycle, inexpensive growth media, and ease of handling of fungi may also contribute to reducing enzyme production costs.
View Article and Find Full Text PDFScience journalists, traditionally, play a key role in delivering science information to a wider audience. However, changes in the media ecosystem and the science-media relationship are posing challenges to reliable news production. Additionally, recent developments such as ChatGPT and Artificial Intelligence (AI) more generally, may have further consequences for the work of (science) journalists.
View Article and Find Full Text PDFThe increase in the generation of chicken feathers, due to the large production of the poultry industry, has created the need to search for ecologically safer ways to manage these residues. As a sustainable alternative for recycling keratin waste, we investigated the ability of the bacterium to hydrolyze chicken feathers and the valorization of the resulting enzymes and protein hydrolysate. In submerged fermentation with three different inoculum sizes (2.
View Article and Find Full Text PDFThe article aims to understand the process through which scientific experts gain and maintain remarkable media visibility. It has been analysed a corpus of 213,875 articles published by the eight most important Italian newspapers across the Covid-19 pandemic in 2020 and 2021. By exploring this process along the different phases of the management of the emergency in Italy, it was observed that some scientific experts achieve high media visibility-and sometimes notwithstanding their low academic reputation-thus becoming a sort of "media star".
View Article and Find Full Text PDFHumic-like substances (HLS) are molecules extracted in an alkaline medium from different materials that have not been subjected to the natural process of humification that occurs in the soil. HLS have the potential to be used as organic fertilizers due to their ability to incorporate micronutrients such as Cu(II) and Co(II); in addition, they represent an alternative for the remediation of contaminated areas due to their high affinity for metals. HLS can be extracted from hydrochar (HC) but only with low yields of approximately 5%.
View Article and Find Full Text PDFFor 2G ethanol production, pentose fermentation and yeast tolerance to lignocellulosic hydrolyzate components are essential to improve biorefinery yields. Generally, physicochemical pre-treatment methodologies are used to facilitate access to cellulose and hemicellulose in plant material, which consequently can generate microbial growth inhibitory compounds, such as furans, weak acids, and phenolic compounds. Because of the unsatisfactory yield of wild-type Saccharomyces cerevisiae during pentose fermentation, the search for xylose-fermenting yeasts tolerant to microbial growth inhibitors has gained attention.
View Article and Find Full Text PDFDescribed is the enhancement of fluorescence intensity due to the interaction of a humic-like substance (HLS 1%) extracted from process water (PW) and Cd(II) ions in aqueous solution. Using Canonical Polyadic/Parallel Factor Analysis (CP/PARAFAC), two main components were seen that contributed to fluorescence, the first one increased it and the second one kept it constant in both static and dynamic fluorescence studies. Two-dimensional FTIR analysis indicated that the interaction of HLS 1% and Cd(II) ions occurred in the following order of affinity with the groups: C-O bonds in polysaccharides > C-O bonds in carboxylic acid.
View Article and Find Full Text PDFJ Biotechnol
March 2022
Xylooligosaccharides (XOs) are a promising class of prebiotics capable of selectively stimulating the growth of the beneficial intestinal microbiota against intestinal pathogens. They can be obtained from xylan present in residual lignocellulosic material from agriculture. Thus, in this study we produced XOs by extracting xylan from sugarcane bagasse and hydrolyzing it using the GH10 xylanase from Thermoascus aurantiacus expressed by Pichia pastoris.
View Article and Find Full Text PDFβ-Glucosidases have been extensively investigated to integrate the enzyme complex for cellulose fiber saccharification and for improving the aroma of wine. To produce these enzymes, greater attention has been given to filamentous fungi and bacteria, and few investigations have targeted the potential applications of enzymes secreted by yeasts. Addressing this issue, in this study, β-glucosidases were produced by the and yeasts, via solid state fermentation with wheat bran as a substrate.
View Article and Find Full Text PDFA future multi-TeV muon collider requires new ideas to tackle the problems of muon production, accumulation and acceleration. In the Low EMittance Muon Accelerator concept a 45 GeV positron beam, stored in an accumulation ring with high energy acceptance and low angular divergence, is extracted and driven to a target system in order to produce muon pairs near the kinematic threshold. However, this scheme requires an intensity of the impinging positron beam so high that the energy dissipation and the target maintenance are crucial aspects to be investigated.
View Article and Find Full Text PDFSucrose methacrylate (SM) is a key monomer for synthesizing biocompatible polymers with a carbohydrate core. However, controlled SM synthesis is challenging due to the possible formation of regio-isomers. This study describes SM synthesis that involves ultrasound irradiation of a homogeneous basic medium.
View Article and Find Full Text PDFKeratinases are proteolytic enzymes with a particular ability to cleave peptide bonds in keratin, and in other proteins. Due to their broad-spectrum of activity, keratinases are considered viable substitutes for chemical and thermal treatments of protein-rich industrial by-products. Among these protein residues, special attention has been given to keratinous materials (feathers, hair, horns, etc.
View Article and Find Full Text PDFDerived compounds from lignin have been used as substrates for chemical and biological processes for obtainment bioproducts. The ferulic acid is a lignocellulosic biomass whose biotransformation in flavors compounds was described. The objective of this study was the bioconversion of ferulic acid to 4-vinylguaiacol by Klebsiella pneumoniae TD 4.
View Article and Find Full Text PDFA protease from the fungus URM 4133, capable of producing bioactive peptides from goat casein, was purified. SDS-PAGE and zymography showed a molecular mass of 30 kDa. The enzyme was active and stable in a wide pH range (6.
View Article and Find Full Text PDFBackground: Animal models and few clinical reports suggest the involvement of the complement system in the onset of severe manifestations of coronavirus disease-2019 (COVID-19). However, complement contribution to endotheliopathy and hypercoagulability has not been elucidated yet.
Objective: To evaluate the association among complement activation, endothelial damage and disease severity or activity in COVID-19 patients.
The main organochlorinated compounds used on agricultural crops are often recalcitrant, affecting nontarget organisms and contaminating rivers or groundwater. Diuron (N-(3,4-dichlorophenyl)-N',N'-dimethylurea) is a chlorinated herbicide widely used in sugarcane plantations. Here, we evaluated the ability of 13 basidiomycete strains of growing in a contaminated culture medium and degrading the xenobiotic.
View Article and Find Full Text PDFBackground: Atrazine is one of the most widespread chlorinated herbicides, leaving large bulks in soils and groundwater. The biodegradation of atrazine by bacteria is well described, but many aspects of the fungal metabolism of this compound remain unclear. Thus, we investigated the toxicity and degradation of atrazine by 13 rainforest basidiomycete strains.
View Article and Find Full Text PDFAppl Biochem Biotechnol
January 2021
Fungal enzymes are widely used in technological processes and have some interesting features to be applied in a variety of biosynthetic courses. Here, free and substrate-immobilised lipases from Fusarium verticillioides P24 were obtained by solid-state fermentation using wheat bran as substrate and fungal carrier. Based on their hydrolytic and transesterification activities, the lipases were characterised as pH-dependent in both reactions, with higher substrate conversion in an alkaline environment.
View Article and Find Full Text PDFBiotechnol Lett
November 2020
Objectives: Keratinases are proteolytic enzymes that emerge as an alternative for dealing with the disposal of chicken feathers. In this study, we aimed to investigate the keratin-degrading enzymes secreted by the fungus Coriolopsis byrsina and their partial biochemical characterization to adapt their use for keratin decomposition, detergent additive applications, and collagen degradation.
Results: We observed the secretion of different proteolytic enzymes that possessed caseinolytic activity that peaked at pH 7.
Lipase stability in organic solvent is crucial for its application in many biotechnological processes as biocatalyst. One way to improve lipase's activity and stability in unusual reaction medium is its immobilization on inert supports. Here, lipases from different sources and immobilized through weak chemical interactions on hydrophobic and ionic supports had their transesterification ability dramatically dependent on the support and also on the solvent that had been used.
View Article and Find Full Text PDFThe present review describes the chemistry and physiological properties of the sucrose esters (SEs) obtained from natural or synthetic pathways, with emphasis on those that have aliphatic and phenylpropanoid substituents on their sucrose moiety. Synthesis, extraction and characterization methods for the SEs and NSEs are discussed in terms of synthetic procedures, separation techniques and spectroscopic methods. The physiological properties are discussed taking into account the nature of the substituent groups and their regiochemistry (position and number of substitutions) on the sucrose moiety.
View Article and Find Full Text PDF