Impacts of ocean acidification and hypoxia on cellular immunity, oxygen consumption and antioxidant status in Mediterranean mussel.

There is growing recognition that the hypoxic regions of the ocean are also becoming more acidic due to increasing levels of global carbon dioxide emissions. The impact of water acidification on marine life is largely unknown, as most previous studies have not taken into account the effects of hypoxia, which may affect how organisms respond to low pH levels. In this study, we experimentally examined the consequences of water acidification in combination with normoxic or hypoxic conditions on cellular immune parameters in Mediterranean mussels.

How water acidification influences the organism antioxidant capacity and gill structure of Mediterranean mussel (Mytilus galloprovincialis, Lamarck, 1819) at normoxia and hypoxia.

The effect of water acidification in combination with normoxia or hypoxia on the antioxidant capacity and oxidative stress markers in gills and hemolymph of the Mediterranean mussel (Mytilus galloprovincialis), as well as on gill microstructure, has been evaluated through an in vivo experiment. Mussels were exposed to a low pH (7.3) under normal dissolved oxygen (DO) conditions (8 mg/L), and hypoxia (2 mg/L) for 8 days, and samples were collected on days 1, 3, 6, and 8 to evaluate dynamic changes of physiological responses.

Effect of specific irradiance on productivity and pigment and protein production of Porphyridium purpureum (Rhodophyta) semi-continuous culture.

Porphyridium purpureum is a promising microalga species due to the content of various valuable compounds. In this study, specific irradiance parameter, representing the amount of light energy per unit of microalgae biomass, was introduced. The growth characteristics and pigments and protein accumulation of P.

Growth and B-Phycoerythrin Production of Red Microalga (Porphyridiales, Rhodophyta) under Different Carbon Supply.

Red microalga (Bory) Drew is a well-known object of biotechnology due to its unique ability to synthesize a wide range of biologically active compounds. Enough minerals in an accessible form in a medium are a prerequisite for maintaining a high growth rate of . Carbon is the main element of microalgal biomass and is a component of all organic compounds.

Determination of Buckling Behavior of Web-Stiffened Cold-Formed Steel Built-Up Column under Axial Compression.

The practice of utilizing cold-drawn steel for structural and non-structural elements has expanded nowadays due to it being lighter in weight, economic section, desirable in fabrication, and its preferred post-buckling behavior over hot rolled sections. The cold-drawn steel section back to the back-lipped channel section has a wide application as a structural member. The fasteners are provided at regular intervals for the long-span structure to prevent individual failures.

Finite Element Analysis of Customized Acetabular Implant and Bone after Pelvic Tumour Resection throughout the Gait Cycle.

The aim of this paper is to investigate and compare the stress distribution of a reconstructed pelvis under different screw forces in a typical walking pattern. Computer-aided design models of the pelvic bones and sacrum made based on computer tomography images and individually designed implants are the basis for creating finite element models, which are imported into ABAQUS software. The screws provide compression loading and bring the implant and pelvic bones together.

Productivity and morphometric parameters of the microalga IBSS-2 under pilot cultivation in continental mid-latitude climate in spring.

In this study, we aimed to investigate the taxonomy and various characteristics of IBSS-2 strain and describe its cultivation potential in mid-latitude climate during springtime. In addition, our analysis confirmed the essentiality of combining morphological, physiological, and other characteristics when identifying new species and strains of the genus , along with the molecular marker (internal transcribed spacer (ITS) of rDNA gene). The pilot cultivation of microalgae during the springtime in the south of Russia demonstrated that the climatic conditions of this region allow cultivation for biomass accumulation during this season, highlighting light and temperature conditions as the main factors determining the growth rate of .

Acute appendicitis caused by : Observations from a case report.

is the most common parasitic worm responsible for gastrointestinal infection worldwide. Acute appendicitis due to is a rare infection, affecting mostly the children. In this report, we present the case of a 5-year-old girl infant infected by found in the appendix lumen.

Immunization of Pigs by DNA Prime and Recombinant Vaccinia Virus Boost To Identify and Rank African Swine Fever Virus Immunogenic and Protective Proteins.

African swine fever virus (ASFV) causes an acute hemorrhagic fever in domestic pigs, with high socioeconomic impact. No vaccine is available, limiting options for control. Although live attenuated ASFV can induce up to 100% protection against lethal challenge, little is known of the antigens which induce this protective response.

Protective antigens against glanders identified by expression library immunization.

Burkholderia are highly evolved Gram-negative bacteria that primarily infect solipeds but are transmitted to humans by ingestion and cutaneous or aerosol exposures. Heightened concern over human infections of Burkholderia mallei and the very closely related species B. pseudomallei is due to the pathogens' proven effectiveness as bioweapons, and to the increased potential for natural opportunistic infections in the growing diabetic and immuno-compromised populations.

Novel Chlamydia pneumoniae vaccine candidates confirmed by Th1-enhanced genetic immunization.

Identification of highly immunogenic antigens is critical for the construction of an efficacious subunit vaccine against Chlamydia pneumoniae infections. A previous project used a genome-wide screen to identify 12 protective C. pneumoniae candidate genes in an A/J mouse lung disease model (Li et al.

Impact of molecular weight in four-branched star vectors with narrow molecular weight distribution on gene delivery efficiency.

A series of star-shaped cationic polymers, termed star vectors (SVs), has been developed as effective nonviral gene delivery carriers. In this study, we separated SVs into several fractions having different molecular weights with very narrow molecular weight distributions in order to examine in detail the influence of the molecular weight of the SVs on the gene transfection efficiency. As a model compound for several types of SVs, 4-branched poly(N,N-dimethylaminopropyl acrylamide) having a molecular weight (M(n)) of approximately 35 kDa and polydispersity of 1.

New classes of orthopoxvirus vaccine candidates by functionally screening a synthetic library for protective antigens.

The licensed smallpox vaccine, comprised of infectious vaccinia, is no longer popular as it is associated with a variety of adverse events. Safer vaccines have been explored such as further attenuated viruses and component designs. However, these alternatives typically provide compromised breadth and strength of protection.

Self-assembled micronanoplexes for improved biolistic delivery of nucleic acids.

A new method for biolistic delivery of nucleic acids using a combination of cationic micro- and nanoparticles is reported. The new method is simpler to perform than the conventional calcium/spermidine-based formulations and shows 11-fold improved nucleic acid binding capacity and dose-dependent performance both for in vitro and in vivo applications relative to either the conventional preparation or our recently reported direct cationic microparticle method. These features may enable higher throughput gene delivery and genetic immunization programs and open new venues for the biolistic delivery method.

Cationic gold microparticles for biolistic delivery of nucleic acids.

Here we report preparation and properties of positively charged gold microparticles, and their use for biolistic DNA delivery. Micron-sized gold microparticles were modified by building self-assembling polyethylenimine monolayers on their surfaces, which enabled their electrostatic interaction with negatively charged molecules such as DNA. One milligram of the surface-modified microparticles was able to bind directly to up to 3.

Mouse model of respiratory Chlamydia pneumoniae infection for a genomic screen of subunit vaccine candidates.

An inbred A/J mouse respiratory challenge model was validated for vaccine testing against Chlamydia (C.) pneumoniae and used to screen the C. pneumoniae genome for vaccine candidates by expression library immunization (ELI).

Organization and transcription of the gene encoding potato UDP-glucose pyrophosphorylase.

The organization of the gene encoding potato UDP-glucose pyrophosphorylase, one of the key enzymes of carbohydrate metabolic pathway is presented. The gene cloned from cultivar (cv.) Lemhi consists of a 6.

A tandemly repeated sequence from the Plasmopara halstedii genome.

A 747-bp tandem repeat element from the genome of the fungus Plasmopara halstedii, the causal agent of downy mildew of sunflower, was cloned and analyzed. The clone can be used as a probe to distinguish races of the pathogen. Sequence analysis of a copy of this element revealed the presence of 103 direct repeats of 6 bp or greater and two potential ORFs.

[Changes in the albumin content of mouse hepatocytes in vitro].

Using the immunoenzyme method followed by the cytophotometrical analysis the dynamics of albumin content changes in isolated mouse hepatocytes in culture was studied. The data obtained point to cyclic changes of the albumin content in the liver cells with the 1 hour periodicity similar to the changes in the rate of protein synthesis revealed for the same cells.

[Sequences homologous to the rat ID element in mouse DNA].

Sequences homologous to the rat frain specific identifier sequences were found in the mouse genome. These sequences were defined by dot- and blotting-hybridisation in different DNA and RNA preparations. It was shown that there are (1-2) X 10(3) copies of these sequences per genome.