The effectiveness of resveratrol in treatment of PCOS on the basis of experimental model in rats.

Purpose Of The Study: Polycystic ovary syndrome (PCOS) is a widespread endocrine disorder in women of reproductive age. Further research is required to justify new directions of effective targeted therapy of this condition. Resveratrol possesses anti-inflammatory, antioxidant and antidiabetic properties.

[Investigation of interaction between silver ions and rutin in water by physical methods].

UV-vis, ASM, AAS, SERS, thermodynamics, kinetics were used to study the reduction of silver ions in excess of rutin to nanoparticles with and without addition of alkali. The reaction consists of a fast redox stage of cluster Ag (1 min) with adsorbed rutin. The secondary stage of a primary nanoparticles ends after 30 minutes.

Does malnutrition in utero determine diabetes and coronary heart disease in adulthood? Results from the Leningrad siege study, a cross sectional study.

Objective: To investigate the relation between decreased maternal food intake and risk factors for coronary heart disease in adult life.

Design: Cross sectional study.

Subjects: 169 subjects exposed to malnutrition in utero (intrauterine group) during the siege of Leningrad (now St Petersburg) in 1941-4; 192 subjects born in Leningrad just before rationing began, before the siege (infant group); and 188 subjects born concurrently with the first two groups but outside the area of the siege (unexposed group).

[Prognostic significance of bicycle ergometry test in patients with myocarditis].

A total of 42 patients with rheumatic carditis were examined in the acute-subacute period and following 3-5 years. Seventeen patients were diagnosed as having primary rheumatic carditis, 9 presented with tonsillogenic rheumatic carditis, and 16 had viral rheumatic carditis. The diagnosis of myocarditis was established on the basis of clinical, immunological, and virological findings.

[The adequacy and safety of low epidural anesthesia in urological operations on patients with concomitant ischemic heart disease].

An electrocardiographic evaluation of the functional state of the myocardium in patients operated upon for urological diseases under conditions of low epidural anesthesia has shown that in the absence of a cardiovascular pathology the method of anesthesia fails to exert a substantial influence on the circulation homeokinesis and functional state of the myocardium. The following use of the low epidural anesthesia in 105 patients with a coexistent ischemic heart disease during urological operative interventions results in two times less amount of cardiovascular complications, lethality and duration of the postoperative period.

[Pregnancy and diabetes mellitus].

The dynamics of the placental lactogenic hormone level, insulin requirements, and glycaemia during pregnancy was studied in diabetes I and II patients and diabetic pregnancies. Fructose amine and C-peptide levels in maternal and neonatal blood were measured in different diabetes types. The risk of diabetes I development in relation to the incidence of the HLA-system antigens was assessed.

[Cytochemical properties of prematurely condensed chromosomes].

Prematurely condensed chromosomes (PCC) have been obtained by polyethylene glycol (PEG) induced fusion in suspension of the Chinese hamster metaphase cultured cells with those in interphase. As alternative approach the PEG-fusion of the Chinese hamster asynchronous culture cells in monolayer with subsequent incubation in free medium was used. A comparative cytofluorimetric investigation of PCC and chromatin of the interphase nuclei of corresponding ploidy has shown some increase (up to 10%) of acridine orange and olivomycin binding with PCC chromatin.

[Fluorescence microscopic study of cells and polykaryons in the early periods following polyethylene glycol treatment].

A fluorescence-microscopical study is made of cultured murine fibroblasts (L-cells) in early periods after the treatment with polyethylene glycol (PEG). Optimal conditions of fusion procedure were found under which the effectiveness of fusion was the highest and the toxical effect of PEG the lowest. The number of dead cells after the treatment with PEG did not exceed 10%.

[Effect of the antitumor antibiotic actinoxanthine on cells cultured in vitro].

Significant changes in the nucleus structure, complete suppression of the mitotic activity, markedly decreased synthesis of RNA (by 70--80 per cent according to incorporation of 3H-uridine) and decreased levels of DNA (by 40 per cent according to olivomycin binding) were observed in the fibroblasts cultivated in vitro due to exposure to actinoxanthine in an amount of 50 microgram/ml. The data indicate direct damaging effect of the drug on the cell chromatin. The above nuclear changes were also observed after a short-term exposure of the cells to the drug (up to 5 minutes).

[Electro- and phonocardiographic changes in tonsillogenic cardiopathies and primary rheumocarditis].

With the use of computers it proved possible to distinguish the most informative electro- and phonocardiographic signs in variants of tonsillogenic affection of the heart and primary rheumatic carditis as the result of which the severity of the myocardial affection could be judged. These signs are very important in the choice of the treatment and in prognosis. Cardiac-type neurocirculatory dystonias are characterized by the fewest informative signs indicative of vegetative effects produced on the heart.

[Use of the antibiotic, olivomycin, for the cytochemical study of chromatin].

The possibility to use the antibiotic olivomycin for fluorescence cytochemistry of chromatin properties has been shown. It is found that the binding of olivomycin to nuclei reveals the functional state of chromatin and changes in the course of its activation. The essential condition for the application of the method described is the use of ethanol-aceton fixative.

[Fluorescence microscopy demonstration of mitochondria in tissue culture cells using berberine].

The possibility of fluorescence microscopical examination of mitochondria in living animal cells using fluorochrome berberine sulphate is shown. At concentrations of 30--50 g per ml the chemical is accumulated selectively in mitochondria of living cells. The specificity of berberine sulphate accumulation in mitochondria was shown by comparative phase contrast and fluorescence microscopy.