Comparison of pcr diagnostic method and mass spectrometry of microbial markers method as applied to the evaluation of oral microbiota.

Rapid informative methods for assessing the species and quantitative composition of the microflora of the periodontal and oropharyngeal covering tissues are necessary for operative diagnostics, including those of the dentofacial system. The use of classical bacteriological methods, including seeding, incubation, counting and identification of microorganisms takes up to 5 days, resulting in a significant delay in obtaining the necessary information, which makes it difficult to carry out operative treatment measures. Therefore, the search for means and methods of operative microbiological control is urgent.

[Liver and Its Lymph Region at Benzo[a]pyrene Effects in an Experiment].

Background: Benzo[a]pyrene (BaP) is the widespread environmental toxicant with carcinogenic activity. BaP undergoes metabolic activation in the liver microsomal monooxygenase system, and its regional lymph nodes act as peripheral filters, purifying lymph formed in the liver. It remains an open question about the significance of the integral liver and lymphatic system work in supporting processes of adaptation and resistance to the BaP effects.

[About the advantages of endovenous laser coagulation of subcutaneous veins].

From February 2007 to March 2010 42 operations concerning the varicose vein disease of lower limbs with magistral valvate inefficiency at the stage of chronic venous insufficiency with the usage of endophleboid laser congelation were performed in clinical outpatient conditions. Diode lasers with wave-length 980 nm. The period of temporary disablement was 14,5 days, after the standard combined phlebectomy with cirsotome--19,5 days.

[Perspectives of improvement of outpatient surgical care in the Armed Forces].

The article presents experience of work of surgical units of the 52nd consultative-diagnostic center of MD RF in ingrafting stationary-staying technologies. In the hospital there were realized all necessary conditions for effectuating a wide range of operations of heightened for policlinic level complexity (hospital register). There is a day patient facility, firstly created in medical service of the Armed Forces in 1989.

[Micro- and ultrastructural characteristics of liver and nuclear endonuclease activity in hepatocytes after the combined exposure to industrial frequency magnetic field and continuous illumination].

The effects of combined action of the industrial frequency magnetic field (MF) and continuous illumination (CI) on microcirculation, ultrastructural features of liver cells and apoptosis were studied in Wistar rats (n=56). It was found that in the liver of rats exposed to MF, CI and CI+MF for 14 days, parenchyma relative area was reduced, and that occupied by the sinusoidal capillaries was increased together with the liver vascularization coefficient. Combined exposure resulted in most pronounced ultrastructural changes (plasma membrane lysis in hepatocytes and endothelial cells, doubling of osmiophilic layers in tight intercellular junctions).

[Morphological criteria of the state of the microcirculation and the lymphatic drainage in the synovial membrane of the knee joint under normal and pathological conditions].

Using transmission electron microscopy, the ultrastructural organization of the endothelium of blood and lymphatic capillaries and the structures of the interstitium of synovial membrane were studied in the patients with osteoarthrosis of a knee joint of I-II and II-III stages. Material, obtained from the patients with traumatic lesions of the knee joint components, was used as a control. The structural signs indicative of the microcirculation and lymphatic drainage disturbances were detected in the synovial membrane and were most expressed in II-III stages of osteoarthrosis.

[Na+,K+,2Cl(-)-cotransport and chloride permeability of the cell membrane in mezaton and histamine regulation of electrical and contractile activity in smooth muscle cells from the guinea pig ureter].

Influence of Na+,K+,2Cl(-)-cotransport and chloride permeability of the cell membrane on electrically-induced action potential and contraction of smooth muscle cells from guinea pig ureter was examined with the methods of the double sucrose gap junction. Mesatone (10 microM) and histamine (10 microM) induced prolongation of the action potential and elevation of smooth muscle cell contraction, whereas hyperosmic medium (+150 mM sucrose), and recovery of solution osmolality in hyposmic condition (70 mM NaCl) after a single contraction. Inhibitor Na+,K+,2Cl(-)-cotransport bumetanide (10 microM) and chloride permeability blockers niflumic acid (10-100 microM) and SITS (10-500 microM) attenuated stimulating effects of mesatone, histamine and hyperosmic medium.

[Preclinical toxicological study of the new enterosorbent noolit].

The safety of the new enterosorbent noolit has been evaluated within the framework of its preclinical characterization. Single introduction of noolit to rats and mice (females and males) in maximum doses for intragastric administration (5.0 - 20.

[Sanatorium rehabilitation of patients with chronic prostatitis].

A total of 75 males from married couples with circulatory prostatitis aged 19-47 were examined and treated in Barnaulsky sanatorium. The patients were trained by walking with the load power 80-110 W for 2.5 to 3 hours.

[Mesenteric lymph nodes in the newborns with inflammatory and non-inflammatory abdominal diseases].

Light microscopy was used to study mesenteric lymph nodes in 46 neonatal infants who had died from small bowel atresia, inflammatory abdominal processes and diseases. There were no lymphoid follicles with germinative centers in the mesenteric lymph nodes of all neonatal infants, which seemed to result from the lack of antigenic stimulation during intrauterine development. Medullary substance malformation in the lymph nodes promotes the occurrence of inflammation in the region of their lymph collection and favors the progression and generalization of a pathological process.

[Lymphatic system and water homeostasis].

Using the methods of light and electron microscopy as well as histochemistry, the complex study of structural-cellular state of the wall of small intestine and its grouped lymphoid nodules, mesenterial and iliac lymph nodes and thymus was performed in rats subjected to the changes of a type of drinking water. Tap, distilled and radon waters were used. The organism was found to respond to the changes in the type of drinking water by both non-specific (increase in sectional area of lymphatic vessels and the number of eosinophilic granulocytes in the wall of small intestine, in lymphoid nodule number containing germinal centers in lymph nodes, in proportion of thymus connective tissue component, increased lymphocyte dehydrogenase activity) and specific reactions, which were characteristic only to a given type of water.

[Regional lymphatic drainage and lymphodetoxication].

Drainage and detoxication functions of lymphatic system at regional level are discussed. Lymphodetoxication is regarded as a unity of biophysical, biochemical and immune actions upon the lymph in lymphoid structures of an anatomical region. Regional lymphatic drainage is considered as a three-component mechanism, that includes interstitial pathways of non-vascular microcirculation, regional lymphatic vessels and lymph nodes.

[Studying cGMP-dependent mechanisms of vinpocetine effect on smooth muscle cells].

The results of the membrane potential measurements (by the double sucrose gap junction technique) and the smooth muscle tension determination (by the mechanical force measurements) in the rat aorta showed that vinpocetine potentiates the effect of sodium nitroprusside and nitroglycerin on the smooth muscle cells. In the concentration range of 2-20 microM, vinpocetine produced a dose-dependent inhibition of the Ca2+ conductivity of the membrane and decreased the smooth muscle contractility response. At a concentration of 1 microM, the drug acted as an inhibitor of the phosphodiestherase (PDE) activity and produced the effects similar top those of dibutyryl-cGMP (rather than dibutyryl-cAMP).

[Myogenic effects of cyclic guanosine monophosphate in smooth muscle cells. Role of protein kinase C].

Heterogeneity of the cGMP-dependent contractility effects of the smooth cells (SMC) was shown in guinea pig ureter by the methods of the double sucrose gap junction. Sodium nitroprusside (SN, 0.1-100 microM) relaxed the high-K+ depolarisationprecontracted SMC but strengthened the SMC constriction triggered by electrical stimulation.

[Mechanisms of NO-dependent relaxation in smooth muscles of the rat aorta with nitro compounds].

The membrane potential and smooth muscle tension in rat aorta were studied by the method of sucrose gap junction. It was found that sodium nitroprusside and nitroglycerin produced a dose-dependent membrane repolarization and smooth muscle cell relaxation in rat aorta preliminarily contracted and depolarized by hyperpotassium (40 mM) or phenylephrine solutions. The relaxation effect of sodium nitroprusside was more pronounced on the phenylephrine background.

[Effect of the enteric sorbent noolit on the psychoemotional status in mice].

The enterosorbent noolit (representing a mineral matrix base with immobilized lithium and silicon compounds) significantly affects the psychoemotional state of mice with depression model induced by prolonged social emotional stress. Noolit administration produces a pronounced anxiolytic and antidepressant effect and reduced the level of behavioral deficiency in the test animals.

[Morphological changes of lymphatic system in patients with the lymphedema of lower extremities].

Morphological changes in inguinal lymph nodes, lymphatic collector and subcutaneous space were studied up in patients with the lower extremities lymphoedema. While the lower extremities lymphoedema occurs in inguinal lymph nodes the connective tissues spreading in two variants is observed. In the first one fibroblasts and collagen fibres appears in medullar substance (chordae medullaris and medullar sinuses) and in the second the sclerosis process begins from cortical plateau and paracortical zone.

[The ultrastructural changes in the cellular lining of lymph node sinuses in organochlorine pesticide poisoning].

In conditions of exotoxicosis arising after the effect of 2,4-dichlorphenoxyacetic acid cytoplasmic cells of littoral cells of marginal and medullary sinuses undergo alike changes and their cell organelles (rough endoplasmic reticulum, Golgi complex, micropinocytotic vesicles and mitochondria) mass is reduced. These changes are more pronounced in littoral cells of marginal sinus when the dos under the effect of 4 permissible doses of the poison. Intensity of intracellular structures reaction to the toxicant action depends on the cell localization on the lymph passage insid within the lymph node (marginal or medullar sinuses).

[The artificial lymph node].

Adsorption of lymphocytes on the sorbents in correction of inflammatory lesions has significant clinical perspectives as the cells and active biological substances they secrete function actively in inflamed tissues acting in the site of its application: every cell adsorbs and inactivates foreign substances, every cytokin affects certain target cells. The products of these cells functioning and dissociation as well as the products of immune system reaction to them remain on the sorbent and are eliminated together with granules during bandaging or by the end of the treatment thus do not entering the organism. Lymphocytic monolayer on the sorbent does not prevent its specific action in pathologic nidus.

[The state of the lymphatic system in inflammation of the internal sexual organs].

In conditions of experimental inflammation of internal sexual organs in female rats fast and adequate reorganization of its lymphatic bed was found. In the nidus of inflammation sufficient drainage of tissues was retained. Loose and widened sinuses of ileal lymph nodes provided sufficient intranodal lymphodynamics.

[The valves of the marginal sinus of the lymph nodes].

Popliteal lymph nodes of male Wistar rats were studied in scanning regime of electron microscope. On the base of the data obtained it is concluded that on the lymph node surface and in the thickness of a capsule afferent lymphatic vessels branch into thin rami supplied with valves from the inside of the capsule in the site of falling into marginal sinus. Valvular apparatus found obviously gives possibility to regulate lymph flow in the lymph node.