Publications by authors named "Borislava Tsafarova"
Visualization of the individual blood microbiome to study the etiology of sarcoidosis.
Comput Struct Biotechnol J
October 2023
Introduction: Single microbial pathogens or host-microbiome dysbiosis are the causes of lung diseases with suspected infectious etiology. Metagenome sequencing provides an overview of the microbiome content. Due to the rarity of most granulomatous lung diseases collecting large systematic datasets is challenging.
View Article and Find Full Text PDFMorphology of blood microbiota in healthy individuals assessed by light and electron microscopy.
Front Cell Infect Microbiol
February 2023
Introduction: The blood microbiome is still an enigma. The existence of blood microbiota in clinically healthy individuals was proven during the last 50 years. Indirect evidence from radiometric analysis suggested the existence of living microbial forms in erythrocytes.
View Article and Find Full Text PDFSequences of multidrug-resistant (MDR) Mycobacterium tuberculosis strains are of particular interest to study the molecular mechanisms of drug resistance evolution. Here, we report the draft genome sequences of 77 endemic multidrug-resistant Mycobacterium tuberculosis strains of SIT41 (TUR) spoligotype from Bulgaria. SIT41 spoligotype is dominant (>40%) among the MDR-TB strains in Bulgaria.
View Article and Find Full Text PDFArticle Synopsis
- Next-generation sequencing (NGS) and metagenomics have transformed our ability to analyze and identify microbial communities in complex samples, confirming the existence of a blood microbiome in healthy individuals but raising questions about the nature of this microbiome as either actual bacteria/fungi or cell-free circulating DNA.
- A study quantitatively evaluated the culturable part of the blood microbiota using a medium enriched with vitamin K and found significant differences in the bacterial and fungal composition between cultured and non-cultured blood samples from healthy individuals.
- Among the findings, non-cultured samples showed a dominance of Proteobacteria, while cultured samples presented a more diverse distribution, revealing a total of 241 bacterial OTUs and 272 fungal OTUs,
bacillus Calmette-Guérin (BCG) is the only live attenuated vaccine available against tuberculosis. The first BCG vaccination was done exactly 100 years ago, in 1921. The BCG vaccine strains used worldwide represent a family of daughter sub-strains with distinct genotypic characteristics.
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