Evaluation of an immunoluminometric assay for the AFP determination.

We evaluated a two-site immunoluminometric assay (AFP LIA-mat byk) for the alpha-fetoprotein (AFP) determination. The assay, involving two monoclonal antibodies which recognize two different epitopes of the AFP, is rapid (approximately 4 h), with a wide working range (0-600 IU/ml) and a good sensitivity of detection. It presents a good reproducibility (CV less than 10% for the total working range).