Fabrication of 3D-printed molds for polydimethylsiloxane-based microfluidic devices using a liquid crystal display-based vat photopolymerization process: printing quality, drug response and 3D invasion cell culture assays.

Microfluidic platforms enable more precise control of biological stimuli and environment dimensionality than conventional macroscale cell-based assays; however, long fabrication times and high-cost specialized equipment limit the widespread adoption of microfluidic technologies. Recent improvements in vat photopolymerization three-dimensional (3D) printing technologies such as liquid crystal display (LCD) printing offer rapid prototyping and a cost-effective solution to microfluidic fabrication. Limited information is available about how 3D printing parameters and resin cytocompatibility impact the performance of 3D-printed molds for the fabrication of polydimethylsiloxane (PDMS)-based microfluidic platforms for cellular studies.

Disability in context: a community-based learning experience.

SUMMARY This article describes a course in disability awareness that integrates didactic content with community-based Level 1 fieldwork experience. Course content includes the experience of disability, effective writing skills, peer review, interviewing techniques, and therapeutic relationships. In home and community settings, students interview and observe volunteers who have disabilities.

Diode laser thermokeratoplasty: application strategy and dosimetry.

Purpose: To investigate suitable application parameters for efficient hyperopic correction by laser thermokeratoplasty (LTK) using mid-infrared laser diodes.

Setting: Medical Laser Center Lübeck, Lübeck, Germany.

Method: A tunable continuous-wave laser diode in the spectral range between 1.

Measuring inhibitory control in children.

The Stop Signal Task is a measure of inhibitory control in which subjects must press a button in response to a stimulus. On certain trials, the subject receives a second stimulus (the Stop Signal) after the primary stimulus and must withhold his/her response during those trials. The onset of the Stop Signal is varied, sometimes coming immediately after the primary stimulus (inhibition is easy); at other times, the Stop Signal arrives quite late, making inhibition difficult.

Quinolones: a practical review of clinical uses, dosing considerations, and drug interactions.

A review of the literature on quinolones reveals numerous clinically relevant points regarding indications, dosing considerations, and drug interactions. Quinolones are useful in the treatment of several infectious diseases. Unfortunately, indiscriminate use of these valuable antimicrobials has resulted in increased patterns of resistance.

Classical conditioning in children with attention deficit hyperactivity disorder (ADHD) and anxiety disorders: a test of Quay's model.

Quay (1988) put forward a model of childhood mental disorders based on Gray's (1982) theory that there exists within the brain a behavioral inhibition system (BIS), which processes signals related to aversive or punishing stimuli. According to this model, children with attention deficit hyperactivity disorder (ADHD) show lower than optimal levels of activity in this system, which leads to less responsiveness at a physiological level to signals related to punishment. Children with ADHD and controls were compared on a classical conditioning paradigm.

Calcium-channel antagonists for prevention of atherosclerosis.

Objective: To critically evaluate the current literature regarding the role of calcium-channel antagonists in preventing atherosclerosis.

Data Sources: English language clinical studies, abstracts, conference proceedings, and review articles pertaining to calcium-channel antagonists and atherosclerosis.

Study Selection: Relevant animal and human studies examining the role of calcium-channel antagonists in atherosclerosis prevention and treatment.

Two- and four-day rifampin chemoprophylaxis regimens induce oxidative metabolism.

The effects of two short-term chemoprophylaxis regimens of rifampin (2 or 4 days) on oxidative metabolism were investigated in 14 healthy subjects. Seven subjects received 600 mg of rifampin twice daily on study days 6 and 7 (group A), and seven subjects received 600 mg of rifampin once daily on days 4, 5, 6, and 7 (group B). Antipyrine (18 mg/kg of body weight) was administered orally on days 1, 8, and 15.

Cardiac sympathetic and parasympathetic activity during self-regulation of heart period.

Fourteen subjects attempted to increase and 15 attempted to decrease cardiac interbeat interval (IBI) while being provided with biofeedback of IBI, T-wave vector magnitude (TWVM), or respiratory sinus arrhythmia (RSA). Subjects in both groups showed directional change in IBI relative to a tracking control task, but the three types of feedback did not differentially affect performance. Voluntary IBI increases were associated with significant increases in TWVM and RSA, and voluntary IBI decreases were associated with significant reductions in RSA and nonsignificant reductions in TWVM.

The use of electromyography and muscle palpation in the diagnosis of tension-type headache with and without pericranial muscle involvement.

The Headache Classification Committee of the International Headache Society recently issued revised diagnostic criteria for headache disorders. According to these criteria, tension-type headache may be subclassified depending upon whether pericranial muscle disorder is found. The presence or absence of pericranial muscle disorder was to be determined by palpating the muscles for tenderness or by measuring electromyographic (EMG) activity.

Update on rifampin drug interactions. II.

Rifampin is a potent inducer of hepatic P450 oxidative enzymes. Clinically important drug interactions have been documented between rifampin and numerous other drugs, such as oral anticoagulants, oral contraceptives, cyclosporine, digitalis, and ketoconazole. New, potentially clinically significant rifampin drug interactions have been reported for haloperidol, several antiarrhythmics, fluconazole, diltiazem, and select benzodiazepines.

Electromyographic and affective responses of episodic tension-type headache patients and headache-free controls during stressful task performance.

Thirty-four subjects meeting diagnostic criteria for episodic tension-type headache and 42 who rarely experienced headaches participated in two laboratory sessions in which cephalic electromyographic (EMG) activity, electrodermal activity, heart rate, and finger temperature were recorded. Subjects performed relaxation, choice reaction time, psychomotor tracking, voluntary muscle contraction, and cold pressor tasks. Headache subjects showed significantly greater EMG activity than controls during baseline and stressful task performance.

A naturalistic study of the relationships among electromyographic activity, psychological stress, and pain in ambulatory tension-type headache patients and headache-free controls.

Twelve subjects who met diagnostic criteria for episodic tension-type headache and nine subjects who rarely or never suffered from headaches wore a computer-controlled electromyographic (EMG) activity recorder in their natural environment for 48 to 96 consecutive hours. EMG activity of the posterior neck or frontal muscles was recorded 24 hr per day. During waking hours, subjects rated their perceived levels of stress, pain, and negative affect at 30-min intervals.

Changes in the T-wave vector loop of the three-dimensional vectorcardiogram during exposure to cold pressor stress.

Three-dimensional vectorcardiography was used to characterize changes in the T-wave vector loop during exposure to cold pressor stress. Data were collected from 8 subjects during baseline, cold pressor, and recovery periods. Maximum vector length, polar angle of the longest vector, azimuth angle of the longest vector, sum of all vectors, polar angle of the vector sum, azimuth angle of the vector sum, surface area of the loop, and maximum distance between adjacent vectors were computed from the T-wave vector loop.

Cardiopulmonary adjustments during operant heart rate control.

Twenty-three healthy men and women participated in a 5-session experiment in which they attempted to increase and decrease their heart rate with the assistance of visual analogue biofeedback. As a group subjects did successfully raise and lower heart rate from resting baseline. These changes in heart rate were closely paralleled by changes in V, a measure of cardiac vagal tone.

Effect of experimental stress on masseter and temporalis muscle activity in human subjects with temporomandibular disorders.

Temporomandibular (TM) disorders have an uncertain aetiology. The purpose of this study was to replicate and extend the work of Yemm (1969) (Archs oral Biol. 14, 873-878, 1437-1439; Br.