Nest site selection and fidelity of European pond turtle (Emys orbicularis) population of Babat Valley (Gödöllő, Hungary).

Background: The conservation of aquatic and semiaquatic turtles requires knowledge of the area and vegetation structure of habitat used for nesting, and nesting migration route. We aimed to survey the effects of habitat features to the nest site selection, nesting success, and test the possibility of nest site fidelity. Our study was carried out at 10 different nesting areas, with special emphasis on data from returning females in a pond system in Hungary between 2014 and 2017.

Additive and dose-dependent mixture effects of Flumite 200 (flufenzin, acaricide) and Quadris (azoxystrobin, fungicide) on the reproduction and survival of Folsomia candida (Collembola).

Chemical plant protection is still a dominant agricultural practice in the world, and usually fields are treated with several pesticides many times per year. This means that not only the single substances affect the environment and the non-target organisms, but their mixtures. Our model organism was Folsomia candida (Collembola).

Urbanization decreases species richness, and increases abundance in dry climates whereas decreases in wet climates: A global meta-analysis.

Soil invertebrates have an essential role in decomposition, nutrient turnover and soil structure formation, all of which are strongly threatened by urbanization. Sealing, compaction by trampling and pollution destroy and degrade city soils and potentially damage soil-living invertebrates. The existing literature on how urbanization affects soil invertebrates is inconsistent, presenting both negative and positive effects.

Aspirin Mediates Its Antitumoral Effect Through Inhibiting PTTG1 in Pituitary Adenoma.

Context: DNA demethylation and inhibitory effects of aspirin on pituitary cell proliferation have been demonstrated.

Objective: Our aim was to clarify the molecular mechanisms behind the aspirin-related effects in pituitary cells.

Methods: DNA methylome and whole transcriptome profile were investigated in RC-4B/C and GH3 pituitary cell lines upon aspirin treatment.

Urbanization hampers biological control of insect pests: A global meta-analysis.

Biological control is a major ecosystem service provided by pest natural enemies, even in densely populated areas where the use of pesticides poses severe risks to human and environmental health. However, the impact of urbanization on this service and the abundance patterns of relevant functional groups of arthropods (herbivores, predators, and parasitoids) remain contested. Here, we synthesize current evidence through three hierarchical meta-analyses and show that advancing urbanization leads to outbreaks of sap-feeding insects, declining numbers of predators with low dispersal abilities, and weakened overall biological pest control delivered by arthropods.

Pollution impacts on water bugs (Nepomorpha, Gerromorpha): state of the art and their biomonitoring potential.

As water pollution poses an increasing risk worldwide, it is timely to assess the achievements of the aquatic macroinvertebrate ecotoxicology to provide a sound basis for the discipline's future and support the development of biomonitoring. Aquatic and semi-aquatic bugs (Hemiptera: Nepomorpha, Gerromorpha) are ubiquitous in almost all water types, sometimes in high densities, and play a significant role in organic material turnover and energy flow. Nevertheless, they are ignored in the water pollution biomonitoring schemes.

A relative quantitation method for measuring DNA methylation and hydroxymethylation using guanine as an internal standard.

Global DNA methylation and hydroxymethylation play an important role in gene expression. They can be connected with several diseases. The modification status could be a biomarker to determine the status of disease.

Three Dimensional Cell Culturing for Modeling Adrenal and Pituitary Tumors.

monolayer conditions are not able to reproduce the complexity of solid tumors, still, there is scarce information about the 3D cell culture models of endocrine tumor types. Therefore, our aim was to develop 3D tumor models by different methodologies for adrenocortical carcinoma (H295R), pituitary neuroendocrine tumor (RC-4B/C and GH3) and pheochromocytoma (PC-12). Various methodologies were tested.

The inter-individual variance can provide additional information for the ecotoxicologists beside the mean.

The hypothesis that the inter-individual parameter variability is an unexploited area of ecotoxicology was proposed several decades ago. Although some illustrative examples were presented to support this hypothesis in the last decades, it has never been tested on an extensive, coherent database. In this study, variance changes of 105 dose-response curves were analysed.

Demethylation Status of Somatic DNA Extracted From Pituitary Neuroendocrine Tumors Indicates Proliferative Behavior.

Background: Cytosine intermediaries 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC), epigenetic hallmarks, have never been investigated in pituitary neuroendocrine tumors (PitNET).

Objective: To examine methylation-demethylation status of global deoxyribonucleic acid (DNA) in PitNET tissues and to assess its correlation with clinical and biological parameters.

Materials And Methods: Altogether, 57 PitNET and 25 corresponding plasma samples were collected.

Comprehensive Analysis of Circulating miRNAs in the Plasma of Patients With Pituitary Adenomas.

Background: Circulating miRNAs in pituitary adenomas would improve patient care, especially as minimally invasive biomarkers of tumor recurrence and progression in nonfunctioning adenoma cases.

Aim: Our aim was to investigate plasma miRNA profiles in patients with pituitary adenomas.

Materials And Methods: A total of 149 plasma and extracellular vesicle (preoperative, early postoperative, and late postoperative) samples were collected from 45 patients with pituitary adenomas.

Transgenerational and multigenerational stress gene responses to the insecticide etofenprox in Folsomia candida (Collembola).

Insecticide exposure may cause both transgenerational and multigenerational effects on populations, but the molecular mechanisms of these changes remain largely unclear. Many studies have focused on either transgenerational or multigenerational mechanisms but did neglect the comparative aspects. This study assessed whether the pyrethroid insecticide etofenprox (formulation Trebon 30 EC) shows transgenerational and/or multigenerational effects on the survival and reproduction of Folsomia candida (Collembola).

Survivin as a potential therapeutic target of acetylsalicylic acid in pituitary adenomas.

Acetylsalicylic acid (ASA) is known as a cancer preventing agent, but there is no data available regarding the effect of ASA on pituitary cells. We investigated 66 nonfunctioning (NFPA) and growth hormone (GH)-producing adenomas and 15 normal pituitary samples. Functional assays (cell viability, proliferation, flow cytometry cell cycle analysis, caspase-3 activation and DNA degradation) were applied to explore the effect of ASA, YM155 (survivin inhibitor), survivin-targeting siRNA and TNF-related apoptosis-inducing ligand (TRAIL) in RC-4B/C and GH3 cells.

New method for automatic body length measurement of the collembolan, Folsomia candida Willem 1902 (Insecta: Collembola).

The collembolan, Folsomia candida, is widely used in soil ecotoxicology. In recent years, growth rate of collembolans has become as frequently used endpoint as reproduction rate in ecotoxicological studies. However, measuring collembolan body sizes to estimate growth rate is a complicated and time-consuming task.