Molecular investigation of staphylococcal cassette chromosome mec types and genotypic relations of methicillin-resistant staphylococci isolated from before and after hospital exposed students.

Background: Reservoir of methicillin resistance genes called staphylococcal cassette chromosome mec (SCCmec), plasmids and genomic characterisations of isolates have been widely investigated in epidemiologic research. However, the extent to which these organisms are transported by patients or hospital staff is not entirely clear.

Aim: This study aims to investigate the molecular relatedness and plasmid profiles of MR staphylococci isolated from nursing students before and after hospital training, to find out the possible source.

[Investigation of intestinal parasites among elementary school students in the Mugla province].

Objective: In this study prevalence of intestinal parasite agents was investigated among elementary school students in the Mugla city centre.

Methods: Only 663 relatives among 3560 students allowed us to investigate and obtain fecal specimens of the students. Specimens were examined by native and lugol staining.

Drug susceptibility testing of Mycobacterium tuberculosis with nitrate reductase assay.

The nitrate reductase assay (NRA) was evaluated for susceptibility testing of Mycobacterium tuberculosis using 80 clinical isolates of M. tuberculosis and H37Rv as a control strain. All isolates were tested by the proportion method and the NRA for isoniazid (INH), rifampicin (RIF), streptomycin (STR) and ethambutol (ETM).

[Investigation of the effects of efflux pump inhibitors on ciprofloxacin MIC values of high level fluoroquinolone resistant Escherichia coli clinical isolates].

In this study, effects of efflux pump inhibitors, phe-arg-beta-naphthylamide (MC-207,110), verapamil, omeprazole and lansoprazole, on ciprofloxacin minimal inhibitory concentrations (MIC) against Escherichia coli clinical isolates with high level fluoroquinolone (FQ) resistance, were evaluated. Fourteen FQ resistant clinical isolates of E. coli isolated from urine samples and identified by BD BBL CRYSTAL Identification Systems Enteric/Nonfermenter ID kit (Becton Dickinson and Company Sparks, USA) were tested.

A multidrug efflux pump inhibitor reduces fluoroquinolone resistance in Pseudomonas aeruginosa isolates.

In general, resistance to fluoroquinolones (FQs) in gram-negative bacteria is acquired either by mutations in DNA gyrase and topoisomerase IV or by active export of the agents via antibiotic efflux pumps. Reduced porin expression is also proposed to be another mechanism leading to resistance. In this study, interaction between levofloxacin, ofloxacin, and ciprofloxacin with MC-207,110 (multidrug efflux pump inhibitor) was investigated by a checkerboard assay using Pseudomonas aeruginosa.

Drug susceptibility testing of Mycobacterium tuberculosis by the broth microdilution method with 7H9 broth.

In this study, we have evaluated the broth microdilution method (BMM) for susceptibility testing of Mycobacterium tuberculosis. A total of 43 clinical isolates of M. tuberculosis and H37Rv as a control strain were studied.

[Antibacterial effect of nitric oxide].

In this study, the antibacterial effects of nitric oxide (NO) have been investigated against 4 strains of multidrug-resistant Mycobacterium tuberculosis and 8 Klebsiella, 7 Escherichia coli, 7 Staphylococcus, 5 Enterobacter, 7 Pseudomonas and 7 Proteus clinical isolates by using DETA-NO ((Z)-1-[N-(2-aminoethyl)-N-(2-ammonioethyl)amino]diazen-1-ium-1,2-diolate) as the NO donor. Minimum inhibitory concentrations (MICs) were determined by broth microdilution method for all tested microorganisms. The MIC values found in our study were as follows; 0.

[In vitro susceptibility of Salmonella enterica serovar Typhimurium to reactive oxygen and nitrogen products].

The aim of this study was to investigate the in-vitro susceptibility of Salmonella enterica serovar Typhimurium against reactive oxygen and nitrogen intermediates, on five different strains. SH5014 as a parental strain, PP120 as a soxRS mutant, SH7616 as a acrAB efflux pump mutant, SZH KUEN 557 as a standard strain and a clinical isolate, were included to the study. Hydrogen peroxide (at 0.

Comparison of the proportion method with mycobacteria growth indicator tube and E-test for susceptibility testing of Mycobacterium tuberculosis.

The aim of this study was to investigate the correlation between proportion method with mycobacteria growth indicator tube (MGIT) and E-test for Mycobacterium tuberculosis. Forty clinical isolates were tested. MGIT and E-test with the first line antituberculous drugs correlated with the proportion method.