Effectors of anterior morphogenesis in C. elegans embryos.

During embryogenesis the nascent Caenorhabditis elegans epidermis secretes an apical extracellular matrix (aECM) that serves as an external stabilizer, preventing deformation of the epidermis by mechanical forces exerted during morphogenesis. At present, the factors that contribute to aECM function are mostly unknown, including the aECM components themselves, their posttranslational regulators, and the pathways required for their secretion. Here we showed that two proteins previously linked to aECM function, SYM-3/FAM102A and SYM-4/WDR44, colocalize to intracellular and membrane-associated puncta and likely function in a complex.

Pathways that affect anterior morphogenesis in embryos.

Unlabelled: During embryogenesis the nascent epidermis secretes an apical extracellular matrix (aECM) that serves as an external stabilizer, preventing deformation of the epidermis by mechanical forces exerted during morphogenesis. We showed that two conserved proteins linked to this process, SYM-3/FAM102A and SYM-4/WDR44, colocalize to intracellular and membrane-associated puncta and likely function together in a complex. Proteomics data also suggested potential roles for FAM102A and WDR44 family proteins in intracellular trafficking, consistent with their localization patterns.

p38-MAPK recruits the proteolytic pathways in Caenorhabditis elegans during bacterial infection.

In eukaryotic organisms, cell-signalling completely relies on Post Translational Modifications (PTMs) that can function as regulatory switches. Phosphorylation is a fundamental and frequently occurring PTM in almost all eukaryotes. Herein, we have studied the importance of protein phosphorylation using classical proteomic techniques in C.

Biomedical Applications and Bioavailability of Curcumin-An Updated Overview.

Curcumin, a yellow-colored molecule derived from the rhizome of , has been identified as the bioactive compound responsible for numerous pharmacological activities of turmeric, including anticancer, antimicrobial, anti-inflammatory, antioxidant, antidiabetic, etc. Nevertheless, the clinical application of curcumin is inadequate due to its low solubility, poor absorption, rapid metabolism and elimination. Advancements in recent research have shown several components and techniques to increase the bioavailability of curcumin.

Physiological and Metabolite Alterations Associated with Neuronal Signals of during Infections.

Metabolomic reprogramming plays a crucial role in the activation of several regulatory mechanisms including neuronal responses of the host. In the present study, alterations at physiological and biochemical levels were initially assessed to monitor the impact of the candidate pathogen on the nematode host . The abnormal behavioral responses were observed in infected worms in terms of hyperosmolarity and high viscous chemicals.

Proteomic analysis of Caenorhabditis elegans against Salmonella Typhi toxic proteins.

Bacterial effector molecules are crucial infectious agents that can cause pathogenesis. In the present study, the pathogenesis of toxic Salmonella enterica serovar Typhi (S. Typhi) proteins on the model host Caenorhabditis elegans was investigated by exploring the host's regulatory proteins during infection through the quantitative proteomics approach.

Salmonella enterica Serovar Typhi exposure elicits deliberate physiological alterations and triggers the involvement of ubiquitin mediated proteolysis pathway in Caenorhabditis elegans.

Involvement of several candidate immune regulatory players at transcriptomic levels during microbial interactions were reported by involving C. elegans as a model system for the past few years. In the present study, we have identified a wide range of phenotypical, physiological and biochemical alterations in C.

Phytol loaded PLGA nanoparticles regulate the expression of Alzheimer's related genes and neuronal apoptosis against amyloid-β induced toxicity in Neuro-2a cells and transgenic Caenorhabditis elegans.

Amyloid β (Aβ) induced neurotoxicity has been postulated to initiate synaptic loss and subsequent neuronal degeneration in Alzheimer's disease (AD). The nanoparticles based drug carrier system is considered as a promising therapeutic strategy to combat this incurable disease. It was also found to inhibit cholinesterase activity and apoptosis mediated cell death in Neuro-2a cells.

Amyloid-β induced neuropathological actions are suppressed by Padina gymnospora (Phaeophyceae) and its active constituent α-bisabolol in Neuro2a cells and transgenic Caenorhabditis elegans Alzheimer's model.

The inhibition of Aβ peptide development and aggregation is a hopeful curative approach for the discovery of disease modifying drugs for Alzheimer's disease (AD) treatment. Recent research mainly focuses on the discovery of drugs from marine setting due to their immense therapeutic potential. The present study aims to evaluate the brown macroalga Padina gymnospora and its active constituent α-bisabolol against Aβ induced neurotoxicity in Neuro2a cells and transgenic Caenorhabditis elegans (CL2006 and CL4176).

Analysis of Caenorhabditis elegans phosphoproteome reveals the involvement of a molecular chaperone, HSP-90 protein during Salmonella enterica Serovar Typhi infection.

Being a primary and prerequisite Post Translational Modification (PTM), protein phosphorylation mediates the defense mechanisms that presides host defense against a pathogen attack. Hence, the current study was intended to uncover the role of regulatory proteins and their PTMs with special attention to phosphorylation during pathogen attack, using C. elegans as a host and S.

In vitro and in vivo biofilm inhibitory efficacy of geraniol-cefotaxime combination against Staphylococcus spp.

In humans, the occurrence of bacterial communities in the form of biofilm is considered as a major intrinsic factor accountable for a variety of stubborn infections. Staphylococcus aureus and S. epidermidis have gained considerable attention in clinical settings owing to the formation of intractable and long-lasting biofilms in medical device.

Synergistic Effect of Quinic Acid Derived From and Undecanoic Acid Against spp. Biofilm and Virulence.

In recent decades, fungal infections have incredibly increased with genus as the major cause of morbidity and mortality in hospitalized and immunocompromised patients. Most of the species are proficient in biofilm formation on implanted medical devices as well as human tissues. Biofilm related infections are very difficult to treat using common antifungal agents owing to their increased drug resistance.

Quorum quelling efficacy of marine cyclic dipeptide -cyclo(L-leucyl-L-prolyl) against the uropathogen Serratia marcescens.

In the current study, the anti-quorum sensing (QS) efficacy of cyclic dipeptide -cyclo(L-leucyl-L-prolyl) (CLP) of marine origin was explored against Serratia marcescens. Minimal -inhibitory (MIC) and -bactericidal concentrations (MBC) of CLP against both reference as well as a clinical isolate of S. marcescens was identified to be 200 and 400 µg/mL, respectively.

-GlcNAcylation confers protection against infection in through ubiquitination.

Glycosylation is one of the most prevalent post-translational modifications in biological systems. In , -GlcNAcylation has been shown to be actively involved in the regulation of dauer formation and detoxification of toxins secreted by invading pathogens. On this backdrop, the present study is focused on understanding the role of -GlcNAcylation in during infection using a gel based proteomic approach.

Green synthesized silver nanoparticles demonstrating enhanced in vitro and in vivo antibiofilm activity against Candida spp.

Candida species are opportunistic fungal pathogens, which are known for their biofilm associated infections on implanted medical devices in clinical settings. Broad spectrum usage of azole groups and other antifungal agents leads to the occurrence of drug resistance among Candida species. Most of the antifungal agents have failed to treat the biofilm mediated Candida infections.

RACK-1, a multifaceted regulator is required for C. elegans innate immunity against S. flexneri M9OT infection.

The nematode C. elegans has the ability to clear off bacterial colonization in the intestine using pathogen specific innate immune response. Here, we show that C.