Expression of VEGFR2 Ligand Binding Domain in Pichia pink™ 4 Cells and Evaluation of Its Interactions with VEGF-A Receptor Binding Domain.

Vascular endothelial growth factor A (VEGF-A) and VEGF receptor 2 (KDR) are important mediators of angiogenesis. We aimed to express the soluble KDR ligand-binding domain (sKDR1-3) and evaluate its interaction with the VEGF-A receptor-binding domain (VEGFA-RBD). sKDR1-3 DNA was designed and subcloned into pPinkα-HC plasmid.

Clodronic Acid has Strong Inhibitory Interactions with the Urease Enzyme of : Computer-aided Design and Confirmation.

Background: (HP) infection could lead to various gastrointestinal diseases. Urease is the most important virulence factor of HP. It protects the bacterium against gastric acid.

Streptomyces strains can improve the quality properties and antifungal bioactivities of tomato fruits by impacting WRKY70 transcription factor gene and nitrate accumulation.

The current study evaluated the effect of plant growth-promoting (PGP) strains of Streptomyces on yield, quality, and nitrate content of fruits, plant-microbe responses, and antifungal effect against blight disease caused by fungus pathogen Alternaria solani on tomato fruits in commercial greenhouse conditions. Greenhouse trials were done with four treatments including strains Y28, IC10, IT25, and commercial bio-fertilizer (Barvar NPK®) on tomato plants. In PGP treatments, the number of infected fruits significantly reduced (60%) compared to Barvar and control.

Data on cell survival, apoptosis, ceramide metabolism and oxidative stress in A-494 renal cell carcinoma cell line treated with hesperetin and hesperetin-7-O-acetate.

Ceramide pathway is a key regulator in cell proliferation and apoptosis and oxidative stress up-regulate ceramides. Acid ceramidase (ACDase), neutral sphingomyelinase (NSMase) and glucosylceramide synthase (GCS) are critical enzymatic systems in ceramide metabolism. Our data represent the comparative assessment of Hesperetin (Hst) and hesperetin-7-O-acetate (HTA) effects on A-494 renal carcinoma cells include cell survival, caspase-3 and 9 activities, total cellular ceramide and the activities of ACDase, NSMase, GCS and superoxide dismutase (SOD).

Inhibition of liver alanine aminotransferase and aspartate aminotransferase by hesperidin and its aglycone hesperetin: An in vitro and in silico study.

Aims: This study aimed to investigate the inhibitory effects of two natural flavonoids, hesperetin (HT) and hesperidin (HD), on two gluconeogenesis enzymes, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities and their possible mechanisms of action.

Main Methods: Rat liver incubated with different concentrations of HT and HD was used to measure enzyme activities spectrophotometrically, based on monitoring the oxidation of NADH to NAD at 340nm. Molecular docking simulation was also applied to reveal the molecular mechanism of the inhibition caused by HT and HD.

Possible involvement of nitrergic and opioidergic systems in the modulatory effect of acute chloroquine treatment on pentylenetetrazol induced convulsions in mice.

Chloroquine has long been used for the treatment of malaria and rheumatological disorders. Accumulating evidence suggests potential use of chloroquine as a neuroprotective agent. Several studies have reported that endogenous opioids and nitric oxide (NO) system mediate the chloroquine effects.

Plasmin digest of κ-casein as a source of antibacterial peptides.

This study investigated the antibacterial properties of plasmin, the plasmin hydrolysis of bovine κ-casein and the fractions (named κC1, κC2, κC3, κC4, and κC5) liberated from it using RP-HPLC. The target bacteria were Escherichia coli, Staphylococcus aureus (pathogenic), Lactobacillus casei and Lactobacillus acidophilus (probiotic). Three peptides (kC1, kC3, and kC4) were found to have antibacterial activity, with κC3 peptide being the most active.

Impact of water deficit stress on biochemical characteristics of safflower cultivars.

Water deficit stress is one of the severe limitations of crop growth especially in arid and semiarid regions of the world as it effect the plant growth at all stages of development. In the present study, four safflower genotypes Esfahan native, Esfahan-14, PI537,598 and IL111 were tested for their growth and crop yield under water-deficit stress. A detailed biochemical analysis was carried out at various levels of irrigation to find out the genotypic variation and the activity of several enzymes known to play significant role under drought stress.

Longitudinal follow-up of oxidative stress and DNA damage parameters in detergent workers.

Background: The aim of this study was the follow-up of work place enzyme and detergent dust exposure effects and smoking habit on DNA damage parameters of workers and the evaluation of their antioxidant enzyme activities and lipid peroxidation with regard to bag-filter installation in the work place.

Material And Methods: All investigated parameters were studied in a group of 153 workers of enzyme-free detergent production plant (E-free) and a group of 138 workers of enzyme-plus detergent plant (E-plus) and compared with 45 controls 7.2 years before and 3.

Level of oxidative stress markers among physicians in a medical residency program.

The authors investigated the effect of engaging in a medical residency program, as a stressful situation, on blood and urine levels of oxidative stress markers. Newly admitted medical residents participated in the study, along with a control group of (nonmedical) students. The authors assessed superoxide dismutase, glutathione peroxidase, catalase, malondialdehyde, micronuclei test, sister chromatid exchange, and 8-hydroxy-2'-deoxyguanosine level.

Comparative evaluation of oxidative stress status and manganese availability in plants growing on manganese mine.

This study pioneered an approach that determined the effects of excess manganese (Mn) on three species; Datura stramonium, Alhagi camelthorn and Chenopodium ambrosioides. We investigated their levels of Mn, antioxidative enzymes and oxidative damage biomarkers in plants (zone 1) in and outside (zone 2) the Mn mine. The results showed that total and available Mn were at toxic levels for plants growing on zone 1.

On the cytotoxicity and status of oxidative stress of two novel synthesized tri-aza macrocyclic diamides as studied in the V79 cell lines.

Two tri-aza macrocycles as diamide derivatives of macrocyclic compounds possess a hydrophilic cavity surrounded by hydrophobic ring, which enables them to diffuse cell membrane and interfere with different living systems. In this study, we comparatively evaluated cytotoxicity effects of tri-aza dibenzo sulfoxide (TSD) and dibenzo sulfide (TTS) macrocyclic diamides in a range of doses (0.5-8mM) and the role of oxidative stress in V79 cell culture.

The copper tolerance strategies and the role of antioxidative enzymes in three plant species grown on copper mine.

This study was undertaken to identify the strategies and the status of antioxidant enzyme activities involved in three plant species tolerance against Cu-toxicity in copper mine. The following methods were used for evaluations in three wild type species; Datura stramonium, Malva sylvestris and Chenopodium ambrosioides. The level of chlorophyll and the activities of superoxide dismutase (SOD), glutathione peroxidase (GPX) and catalase (CAT) by spectrometry, malondialdehyde (MDA) and dityrosine by HPLC and the levels of Cu in tissues and soils by atomic absorption spectrometry (AAS).

Organ culture studies on the development of mouse embryo limb buds under EMF influence.

Purpose: To investigate the effects of an electromagnetic field (EMF) on limb bud development in vitro, an organ culture system was applied.

Materials And Methods: Three test groups of amputated mouse limb buds included the experimental (E) group which received EMF (50 Hz/13.1 mT, for 2 h), a sham (Sh) group exposed to no EMF treatment and the control (C) group.

Development and reduction of hypertension and oxidative stress among detergent industry workers.

Hypertension status and oxidative stress parameters were assessed in 291 workers (hypertensive workers were divided into three grades, non-equivalently) at two detergent production plants, one of which included enzymes in the detergent (n=138) and another which did not (n=153), and 45 control workers in another industry three times (at the time of employment, 7 yrs later at the time of installation of a filter system, and about 3 yrs later). Malondialdehyde (MDA) was measured by high-performance liquid chromatography, antioxidant enzymes and lipid status by ultraviolet-visible spectrophotometry, trace elements by atomic absorption spectroscopy, and blood pressure using an oscilometric device. Prior to filter system installation, enzyme-exposed workers had significantly higher MDA, antioxidant enzyme activities, and prevalence of hypertension, compared with controls.

Oxidative response of rat lung tissue after crown ethers exposure and the effects of alpha-tocopherol treatment.

Background: Ionophoric properties of crown ethers enable them ideally to transport across membranes and interfere with different living systems. We studied cytotoxicity effects of 18-crown-6 and 15-crown-5 and the role of oxidative stress in rat lung tissue culture.

Methods: We assayed the effects of these crown ethers in a range of doses (0.

Cytotoxicity and the levels of oxidative stress parameters in WI38 cells following 2 macrocyclic crown ethers treatment.

Background: Crown ethers as macrocyclic polyethers possess a hydrophilic cavity surrounded by hydrophobic ring which enable them to diffuse cell membrane. We evaluated cytotoxicity effects of 15-crown-5 and 18-crown-6 and the role of oxidative stress in WI38 cells culture.

Methods: The effect of these ethers in a range of doses (0.

Long-term follow-up of workplace and well water manganese effects on iron status indexes in manganese miners.

The authors assessed the effect of water reconstitution in the workplace by evaluating the iron status of manganese mine workers during a long-term study. Subsequent analyses and biological monitoring were performed in a group of 150 manganese miners before, and 2.8 yr after, reconstitution of drinking water in the miners' workplace.

A longitudinal follow-up of pulmonary function and respiratory symptoms in workers exposed to manganese.

The purpose of the investigation was to study the effects on the respiratory system in mine workers with long-term exposure to manganese (Mn) in the workplace. The study included a follow-up of pulmonary function and respiratory symptoms among 145 workers employed in a large Mn mine and 65 matched controls, and the assessment of Mn concentrations in environment and biological samples. Lung function was measured by recording spirometric parameters.

The effect of indomethacin on colony-stimulating-factor production by the lung.

In this study, indomethacin was used to investigate the production of colony-stimulating-factor by the lung tissue. Addition of various concentrations of indomethacin (10(-5)-1 mg/ml) to the lung culture showed that it enhances CSF production in a dose dependent manner. The number of colonies reached a maximum at 1 microgram/ml and gradually diminished at higher concentrations.