Clinical identification and endodontic management of furcation canals: a case series.

In the case of endodontic infection, the presence of furcation canals can be at the origin of a periodontal lesion located in the furcation. The furcation being very close to the marginal periodontium, this type of lesion can be favorable to the genesis of an endo-periodontal lesion. These furcation canals are lateral canals located on the pulp chamber floor and constitute one of the many physiological communication pathways between endodontic and periodontal tissues.

Acute appendicitis caused by : Observations from a case report.

is the most common parasitic worm responsible for gastrointestinal infection worldwide. Acute appendicitis due to is a rare infection, affecting mostly the children. In this report, we present the case of a 5-year-old girl infant infected by found in the appendix lumen.

Endoperiodontal Lesions and Chicago's New Classification of Periodontal and Peri-implant Diseases and Conditions.

Aim: To illustrate, with two clinical cases of endoperiodontal lesions, the clinical application of the new classification of periodontal and peri-implant diseases and conditions.

Background: The endodont and the periodont are two entities that communicate with each other through physiological communication channels (apical foramen, lateral and secondary canals, and dentinal tubules) resulting in close anatomical and functional interaction. An endoperiodontal lesion is defined by pathological communication between the endodontic and periodontal tissues in a given tooth, according to the definition given by the new classification of periodontal and peri-implant diseases and conditions from the work of the Chicago Consensus Conference in 2017.

Performance of Fluorescence-based Systems in Early Caries Detection: A Public Health Issue.

Aim: Modern clinical caries management involves early stage caries diagnosis and should fit with dental health policy. The objective of this study was to achieve early caries detection in enamel and dentine with a laser-based system (DIAGNOdent™ pen) first and secondary with a new fluorescence intra-oral camera (Soprolife®). A visual inspection with a loupe was used as control.

Adjuvant therapeutic potential of tonabersat in the standard treatment of glioblastoma: A preclinical F98 glioblastoma rat model study.

Purpose: Even with an optimal treatment protocol, the median survival of glioblastoma (GB) patients is only 12-15 months. Hence, there is need for novel effective therapies that improve survival outcomes. Recent evidence suggests an important role for connexin (Cx) proteins (especially Cx43) in the microenvironment of malignant glioma.

Heat-moulded versus custom-made mandibular advancement devices for obstructive sleep apnoea: a randomised non-inferiority trial.

Rationale: Custom-made mandibular advancement devices (MADs) are reported as providing higher efficacy rates compared with thermoplastic heat-moulded MADs but at the price of higher costs and treatment delays.

Objective: To determine whether a thermoplastic heat-moulded titratable MAD (ONIRIS; ONIRIS SAS, Rueil Malmaison, France) is non-inferior to a custom-made acrylic titratable MAD (TALI; ONIRIS SAS, Rueil Malmaison, France) for obstructive sleep apnoea (OSA).

Methods: We conducted a multicentre, open, randomised controlled trial of patients with OSA refusing or not tolerating continuous positive airway pressure (CPAP).

In vitro effects of two silicate-based materials, Biodentine and BioRoot RCS, on dental pulp stem cells in models of reactionary and reparative dentinogenesis.

Background: Calcium silicate-based cements are biomaterials with calcium oxide and carbonate filler additives. Their properties are close to those of dentin, making them useful in restorative dentistry and endodontics. The aim of this study was to evaluate the in vitro biological effects of two such calcium silicate cements, Biodentine (BD) and Bioroot (BR), on dental stem cells in both direct and indirect contact models.

Effects of High-Temperature-Pressure Polymerized Resin-Infiltrated Ceramic Networks on Oral Stem Cells.

Objectives: The development of CAD-CAM techniques called for new materials suited to this technique and offering a safe and sustainable clinical implementation. The infiltration of resin in a ceramic network under high pressure and high temperature defines a new class of hybrid materials, namely polymer infiltrated ceramics network (PICN), for this purpose which requires to be evaluated biologically. We used oral stem cells (gingival and pulpal) as an in vitro experimental model.

Cognitive impairment in a young marmoset reveals lateral ventriculomegaly and a mild hippocampal atrophy: a case report.

The number of studies that use the common marmoset (Callithrix jacchus) in various fields of neurosciences is increasing dramatically. In general, animals enter the study when their health status is considered satisfactory on the basis of classical clinical investigations. In behavioral studies, variations of score between individuals are frequently observed, some of them being considered as poor performers or outliers.

MTA versus Ca(OH)2 in apexification of non-vital immature permanent teeth: a randomized clinical trial comparison.

Objectives: The aim of this prospective randomized clinical trial was to compare mineral trioxide aggregate (MTA) with calcium hydroxide (CH) as materials for inducing root apex closure in immature necrotic permanent incisors.

Methods: The design of this study has been extensively described in the authors' previous report. Children (n = 30), aged from 6 to 18 years and presenting a non-vital permanent incisor, were treated.

Outcome of endodontic treatments made by postgraduate students in the dental clinic of bretonneau hospital.

Objective. The objective of this retrospective study is double: (1) to assess the 1-4 years of outcome of endodontic treatment performed by postgraduate students in endodontics in the Dental Clinic of Bretonneau Hospital and (2) to examine outcome predictors. Method.

Age effects on transfer index performance and executive control in baboons (Papio papio).

Reversal performance in the transfer index (TI) task is known to improve from prosimians to apes, suggesting that this task is a marker of cognitive evolution within the primate taxa (Rumbaugh, 1970). However, the cognitive processes recruited by this task remain unclear. In the present study, 19 socially-housed baboons (Papio papio) from 1.

Management of internal root resorption on permanent teeth.

Internal root resorption (IRR) is a particular category of pulp disease characterized by the loss of dentine as a result of the action of clastic cells stimulated by pulpal inflammation. This review article explains the etiology, the prevalence of IRR, and, in addition to the clinical data, the contribution of the three-dimensional imaging (CBCT) to the diagnosis, the clinical decision, and the therapeutic management of IRR. The authors discussed the various therapeutic options including the orthograde or retrograde fillings of the root canal resorption area.

Minimal intervention dentistry: part 6. Caries inhibition by resin infiltration.

Resin infiltration has made possible an innovative way of treating initial carious lesions that fits perfectly with the concept of minimal intervention dentistry. Infiltration of carious lesions represents a new approach to the treatment of non-cavitated lesions of proximal and smooth surfaces of deciduous and permanent teeth. The major advantage of this method is that it is a non-invasive treatment, preserving tooth structure and that it can be achieved in a single visit.

Minimal intervention dentistry: part 4. Detection and diagnosis of initial caries lesions.

The detection of carious lesions is focused on the identification of early mineral changes to allow the demineralisation process to be managed by non-invasive interventions. The methods recommended for clinical diagnosis of initial carious lesions are discussed and illustrated. These include the early detection of lesions, evaluation of the extent of the lesion and its state of activity and the establishment of appropriate monitoring.

Age-dependent behavioral strategies in a visual search task in baboons (Papio papio) and their relation to inhibitory control.

A computerized visual search task was presented to 18 guinea baboons (Papio papio) ranging from 2.7 to 14.3 years of age.

Mineral trioxyde aggregate versus calcium hydroxide in apexification of non vital immature teeth: study protocol for a randomized controlled trial.

Background: Pulp necrosis is one of the main complications of dental trauma. When it happens on an immature tooth, pulp necrosis implies a lack of root maturation and apical closure. A therapy called apexification is required to induce the formation of a calcified apical barrier allowing a permanent and hermetic root filling.

Executive control of perceptual features and abstract relations by baboons (Papio papio).

Studies of executive control often reveal significant limitations in nonhuman primate performance relative to that of humans. In the present study, 24 socially housed baboons were tested on a computerized version of the Wisconsin Card Sorting Task (WCST) to assess individual differences in executive control. In a second experiment, the same baboons completed a version of the WCST with paired-relational stimuli rather than those that varied on a single dimension to evaluate their set-shifting abilities with abstract relations (same/different).

Automated testing of cognitive performance in monkeys: use of a battery of computerized test systems by a troop of semi-free-ranging baboons (Papio papio).

Fagot and Paleressompoulle (2009) published an automated learning device for monkeys (ALDM) to test the cognitive functions of nonhuman primates within their social groups, but the efficiency of the ALDM procedure with large groups remains unknown. In the present study, 10 ALDM systems were provided ad lib to a troop of 26 semi-free-ranging baboons that were initially naive with computerized testing. The test program taught baboons to solve two-alternative forced choice (2AFC) and matching-to-sample (MTS) tasks.

Prevalence of apical periodontitis in root canal-treated teeth from an urban French population: influence of the quality of root canal fillings and coronal restorations.

This cross-sectional study determined the prevalence of apical periodontitis in 1035 root canal-treated teeth from adult French patients and investigated the influence of the quality of canal fillings and coronal restorations on the periradicular status. Periapical radiographs were used for analyses, and teeth were classified as healthy or diseased according to the periapical index scoring system. Overall, the prevalence of apical periodontitis in root canal-treated teeth was 33%.

Preparation of chromatin assembly extracts from preblastoderm Drosophila embryos.

A rigorous biochemical analysis of chromatin structure and function requires the assembly of chromatin in vitro. A useful alternative to reconstituting nucleosomal arrays from pure or recombinant histones by salt gradient dialysis is the assembly of more complex chromatin from assembly extracts under physiological conditions. Extracts from preblastoderm embryos have proven to be particularly efficient, due to the presence of large stores of native complexes of histones, histone chaperones and ATP-dependent nucleosome spacing factors.

Concept of uprightness in baboons: assessment with pictures of realistic scenes.

How nonhuman primates process pictures of natural scenes or objects remains a matter of debates. This issue was addressed in the current research by questioning the processing of the canonical orientation of pictures in baboons. Two adult guinea baboons were trained to use an interactive key (IK) on a touch-screen to change the orientation of target pictures showing humans or quadruped mammals until upright.

Isolation of transcription factor complexes by in vivo biotinylation tagging and direct binding to streptavidin beads.

Efficient tagging methodologies are an integral aspect of protein complex characterization by proteomic approaches. Owing to the very high affinity of biotin for avidin and streptavidin, biotinylation tagging offers an attractive approach for the efficient purification of protein complexes. The very high affinity of the biotin/(strept)avidin system also offers the potential for the single-step capture of lower abundance protein complexes, such as transcription factor complexes.

GATA-1 forms distinct activating and repressive complexes in erythroid cells.

GATA-1 is essential for the generation of the erythroid, megakaryocytic, eosinophilic and mast cell lineages. It acts as an activator and repressor of different target genes, for example, in erythroid cells it represses cell proliferation and early hematopoietic genes while activating erythroid genes, yet it is not clear how both of these functions are mediated. Using a biotinylation tagging/proteomics approach in erythroid cells, we describe distinct GATA-1 interactions with the essential hematopoietic factor Gfi-1b, the repressive MeCP1 complex and the chromatin remodeling ACF/WCRF complex, in addition to the known GATA-1/FOG-1 and GATA-1/TAL-1 complexes.