Construction and Characterization of a Full-Length Infectious cDNA Clone of a Strain of Watermelon Mosaic Virus Isolated from Melon.

Watermelon mosaic virus (WMV), a member of the genus Potyvirus, causes serious economic losses in cucurbit crops. For molecular biological studies of viruses, it is necessary to construct an infectious clone that can facilitate gene functional analysis. In this study, we constructed an infectious cDNA clone of WMV genomic RNA by Gibson assembly and evaluated its virulence and symptoms on a variety of host plants.

First report of beet western yellows virus in radish in Korea.

Radish ( L.) is an important root vegetable widely consumed in kimchi in Korea. In October 2021, radish leaves with virus-like symptoms of mosaic and yellowing were collected in three fields around Naju, Korea (Fig.

Complete Genome Sequence of a Novel Monopartite , Soybean Geminivirus B, Isolated from Soybean ( (L.) Merrill).

Soybean is one of the most important crops in Korea. To identify the viruses infecting soybean, we conducted RNA sequencing with samples displaying symptoms of viral disease. A contig displaying sequence similarity to the known was identified.

Development of a transcranial direct current stimulation (tDCS) device based on polarity interchangeable electrodes.

Background: Transcranial direct current stimulation (tDCS) is a noninvasive brain stimulation technique that can help modulate cortical excitability by transmitting direct current (DC) between a pair of scalp electrodes. To date, most studies on tDCS have been relatively short-lived, and the DC stimulations only lasted a few minutes. Conventional tDCS devices usually have some problems such as needing a lot of patches and lead lines.

The Development of Simple Methods for the Maintenance and Quantification of .

, a root endoparasite of several cereal species, is considered to be non-pathogenic but serves as a vector of various plant viruses belonging to the genera , , and . Specifically, it reduces barley productivity by transmitting the (BaYMV). To date, due to its obligate biotrophic property, no artificial culturing of was reported and its quantification was also technically challenging.

A systems approach for identifying resistance factors to Rice stripe virus.

Rice stripe virus (RSV) causes disease that can severely affect the productivity of rice (Oryza sativa). Several RSV-resistant cultivars have been developed. However, host factors conferring RSV resistance in these cultivars are still elusive.

Suppression of NS3 and MP is important for the stable inheritance of RNAi-mediated rice stripe virus (RSV) resistance obtained by targeting the fully complementary RSV-CP gene.

Rice stripe virus (RSV) is a viral disease that seriously impacts rice production in East Asia, most notably in Korea, China, and Japan. Highly RSV-resistant transgenic japonica rice plants were generated using a dsRNAi construct designed to silence the entire sequence region of the RSV-CP gene. Transgenic rice plants were inoculated with a population of viruliferous insects, small brown planthoppers (SBPH), and their resistance was evaluated using ELISA and an infection rate assay.

G7H, a New Soybean mosaic virus Strain: Its Virulence and Nucleotide Sequence of CI Gene.

A new Soybean mosaic virus (SMV) strain was isolated in Korea and designated as G7H. Its virulence on eight differentials and 42 Korean soybean cultivars was compared with existing SMV strains. G7H caused the same symptoms as G7 did on the eight differential cultivars.

Molecular characterization of the gene encoding rice allene oxide synthase and its expression.

The gene encoding rice allene oxide synthase, OsAOS, was intronless and had nucleotide sequences with the high GC content of 67%. Deduced amino acid sequences had very high similarity with other AOS proteins, in particular 74% similarity to barley, characterized by the conserved motifs of P450 cytochrome of the CYP74A family. Purified recombinant rice AOS protein expressed in Escherichia coli converted 13-hydroperoxylinolenic acid to allene oxide.