[Effect of mold fungus spore consumption with food on systemic anaphylaxis in rats].

An influence was studied in experiment of intragastrically administrated milk cream contaminated with mould fungi spores on systemic anaphylactic reaction gravity in Wistar albino rats sensitized with egg albumin (EA). During 28 days sensitized rats intragastrically received cream containing 10(3) or 10(6) colony forming units of 5 fungal species in one cm3. After being challenged rats developed mild anaphylactic shock followed by elevation of small intestinal permeability for macromolecular tracer polyethylene glycol 4000.

[The antagonism of the natural microflora of grain as a regulating factor in Fusarium sporotrichiella toxin formation].

The ability of 32 fungi species of 9 genera that belong to field grain microflora to inhibit the growth of F. sporotrichiella strain 53315 producing T2 toxin has been studied. Antagonistic properties have been found in representatives of Epicoccum and Drechslera genera.

[The search for microorganisms sensitive to deoxynivalenol, zearalenone and aflatoxin B1].

Sensitivity to T-2 toxin, deoxynivalenol (DOV), zearalenon (ZL) and aflatoxin (AT) B1 was comparatively studied in yeast strains Saccharomyces fragilis 25 D, Candida pseudotropicalis 44 nk and Saccharomyces lactis BKMY-459, sensitive to T-2 toxin, and strain Bacillus megaterium BKMB-44, sensitive to toxic metabolites of Aspergillus species of fungi. Minimum amounts of T-2 toxin inducing on Silufol plates sites of the yeast strain growth suppression, comprised 20-50 ng, maximum sensitivity was recorded in Saccharomyces lactis BKMY-459. Minimum content of DOV suppressing the yeast growth comprised 10-50 micrograms, and that of ZL 10-25 micrograms.

[Detection of toxigenic Fusarium strains, producing T-2 toxin, in wheat grain mycoflora by microbiologic assay].

Twenty-three Fusarium strains were isolated from wheat grain harvested in the Moscow region. The ability of the fungi cultures isolated for producing T-2 toxin was studied by the microbiological assay with the use of Saccharomyces lactis culture (BKMU-459) susceptible to T-2 toxin. The toxigenic properties were shown by 9 cultures.

[Characteristics of the action of extracts of pink-stained grain of fusarial and nonfusarial origin on the structure of the organs in growing rat pups].

The characteristics of the toxic effect of rose-stained grain of fusarial and non-fusarial nature, as well as the grain experimentally challenged with the toxic strain Fusarium sporotrichiella 53315, were studied basing on the analysis of morphological changes in the internal organs of young rat given orally extracts from the samples studied. The tissue aging processes under the effect of the extract doses producing no irreversible pathologic changes were investigated. It was shown that the extracts of rose-stained natural grain, fusarial and without signs of fusaridiosis, produced similar pathological changes in the morphological structures of the organs in the young rats, the toxicity grew with the increase of the per cent of rose-stained grain.

[Origin of the pinkish coloration of rye grain].

The grain requires pink coloration while in the fields during grain ripening in the stage of picking maturity with this coloration being absent in the stage of gold ripeness. Of the microorganisms that form pink, red or orange pigments, Fusarium, Trichothecium roseum and Epicoccum, were detected to reveal high degree of fungal affection, particularly with Fusarium, of freshly harvested pink-colored grain and grain in the stage of gold ripeness during rainy and cool summer. It was demonstrated that upon grain storage the fungi Fusarium rapidly disappear and are detected in far less quantities on mycological analysis.

[Dynamics of B1 aflatoxin and Aspergillus flavus spore formation on rye and wheat grain].

Rye and wheat grain was infected with Aspergillus flavus spores. The dynamics of B1 aflatoxin and spore formation was studied. The synthesis of B1 aflatoxin in the mold-affected grain started simultaneously with the emergence of fungal spores; its further accumulation in the grain correlated with an increase in the spore number till the stage of maximum spore formation.