Biallelic Loss of 7q34 () and 9p21.3 (/) in Adult Ph-Negative Acute T-Lymphoblastic Leukemia.

Tumor cells of acute lymphoblastic leukemia (ALL) may have various genetic abnormalities. Some of them lead to a complete loss of certain genes. Our aim was to reveal biallelic deletions of genes in Ph-negative T-ALL.

Morphological Reconstruction of a Critical-Sized Bone Defect in the Maxillofacial Region Using Modified Chitosan in Rats with Sub-Compensated Type I Diabetes Mellitus.

It is known that complexes based on natural polysaccharides are able to eliminate bone defects. Prolonged hyperglycemia leads to low bone regeneration and a chronic inflammatory response. The purpose of this study was to increase the efficiency of early bone formation in a cavity of critical size in diabetes mellitus in the experiment.

Experimental Early Stimulation of Bone Tissue Neo-Formation for Critical Size Elimination Defects in the Maxillofacial Region.

A biomaterial is proposed for closing extensive bone defects in the maxillofacial region. The composition of the biomaterial includes high-molecular chitosan, chondroitin sulfate, hyaluronate, heparin, alginate, and inorganic nanostructured hydroxyapatite. The purpose of this study is to demonstrate morphological and histological early signs of reconstruction of a bone cavity of critical size.

[Nutritional factors of bone health in athletes].

The importance of proper nutrition when playing sports is difficult to overestimate. Athletes of any age need to consume a sufficient amount of macro- and micronutrients for bone health. High-quality and balanced nutrition in terms of quantity and composition is important for optimal recovery after training, adaptation to intense physical activity and prevention of sports injuries.

Synthesis, Chemical and Biomedical Aspects of the Use of Sulfated Chitosan.

This work is devoted to the chemical synthesis of sulfated chitosan and its experimental verification in an animal model of early atherosclerosis. The method of chitosan quaternization with sulfate-containing ingredients resulted in a product with a high content of sulfate groups. Implantation of this product into the fascial-muscular sheath of the main limb artery along the leg and thigh in rabbits led to the extraction of cholesterol from the subintimal region.

[Relative energy deficiency in sport: modern approaches to diagnostics, treatment and prevention].

In recent years, close attention has been paid to energy deficiency in professional athletes. More and more studies confirm the widespread prevalence of relative energy deficiency syndrome in sports and its relationship with various pathological conditions that lead to a decrease in the level of athletic performance. Nowadays the possibilities of early diagnosis and treatment of this syndrome are being carefully studied, and clinical protocols are being actively developed to facilitate the early detection of energy deficiency.

Optical interference coating design contest 2016: a dispersive mirror and coating uniformity challenge.

A dispersive mirror and a coating uniformity challenge were the topics of the design contest held in conjunction with the 2016 Optical Interference Coatings topical meeting of The Optical Society (OSA). A total of 18 designers from China, France, Germany, Japan, and the United States submitted 38 total designs for problems A and B. Michael Trubetskov submitted the winning designs for all four design challenges.

Morphological study of burn wound healing with the use of collagen-chitosan wound dressing.

Experiments on the model of thermal skin burn in rats showed that the use of wound dressing based on collagen-chitosan complex Kollakhit-Bol in local treatment of grade IIIb skin burns increased healing rate by accelerating the formation of granulation and fibrous connective tissues and reducing crust thickness in comparison with Kollakhit coating. Kollakhit-Bol provided targeted stimulation of reparative processes in the treatment of grade IIIb burns by creating favorable conditions for grafting full thickness skin transplant or dermal-epidermal skin equivalent. In the topical treatment of thermal burn, Kollakhit-Bol application shortened the phases of alteration and exudation and accelerated transition to the productive phase of the inflammatory process with phagocytosis and neoangiogenesis activation.

Mouse hepatocytes retain the expression of the main differentiation markers during culturing on collagen-chitosan matrices.

Mouse hepatocytes cultured on artificial 3D collagen-chitosan biopolymer matrices retained the expression of hepatocyte markers for 14 days.

Catalase activity of cytochrome C oxidase assayed with hydrogen peroxide-sensitive electrode microsensor.

An iron-hexacyanide-covered microelectrode sensor has been used to continuously monitor the kinetics of hydrogen peroxide decomposition catalyzed by oxidized cytochrome oxidase. At cytochrome oxidase concentration ~1 µM, the catalase activity behaves as a first order process with respect to peroxide at concentrations up to ~300-400 µM and is fully blocked by heat inactivation of the enzyme. The catalase (or, rather, pseudocatalase) activity of bovine cytochrome oxidase is characterized by a second order rate constant of ~2·10(2) M(-1)·sec(-1) at pH 7.

[Use of liquid sorbents based on chitosan for treatment of diffuse forms of peritonitis].

Chitosan-based gelatinous affinity sorbent containing 9 mg/ml of polymyxin B was injected into the infected peritoneal cavity for twenty-four hours for detoxification in diffuse peritonitis. The clinical assay of blood plasma (Spectrum Abbot, USA) revealed that the lavage of the infected abdomen by polymyxin B-containing adsorbent resulted in systemic detoxification. The effect was associated with normalization of direct bilirubin, cholesterol, and triglyceride levels, a 4-fold decrease in uric acid levels, and a 34-fold increase in alkaline phosphatase activity, and a greater than 70-fold enhancement of hepatic aspartate aminotransferase activity.

[Lavage of the infected peritoneal cavity using agarose-based immunoaffinity gel sorbents].

In the paper antistaphylococcus and polymixine liquid sorbents are used to lavage infected rat abdominal cavity against gram-positive and gram-negative microfloral toxins. Sorbents have been revealed to remove erythrocyte antigens from the surface, that are affine to immobilized ligands on the matrix. Polymyxine affine sorbent arises quality of the usual lavage of the infected abdomen.

[The immunocorrective potentials of an antistaphylococcal immunosorbent in extracting staphylotoxin].

The immunocorrective effect of antistaphylococcal adsorbent prepared on the basis of silochrome, a silica matrix, has been revealed in experiments on peripheral blood lymphocytes obtained from 18 donors. The expression of receptors on human immunoregulating cells has been suppressed by the addition of staphylotoxin at a toxic concentration into the system. The experiments have shown that immunosorption is capable of extracting the toxin from the solution to a considerable degree, thus preventing the suppression of the receptor apparatus of immunoregulating lymphocytes.

[The affinity lavage of the abdominal cavity in diffuse peritonitis with liquid sorbents based on cross-linked dextrans].

Experiments were conducted on 35 Wistar rats with generalized purulent peritonitis. Twenty-four hours after infection the authors introduced into the animals' abdominal cavity gels of cross-linked dextrans with immobilized polymixin (50 mg/g dry weight) and terrilithin (100 PE/g dry weight), as well as a combination of these sorbents with 24-hour exposure in a 5 ml volume. The object of the study was the detoxification effect.

[Torsion of the colonic epiploic appendages].

Operations were carried out on 21 patients with torsion of epiploic appendages of the colon (sigmoid 19, ascending 1, hepatic flexure 1). This pathologic condition has no specific manifestations and occurs under the mask of other emergencies. The suggested symptom of "pain displacement" in combination with other signs allowed the correct diagnosis to be established before the operation in two thirds of patients.

[The immunomodulating properties of staphylotoxin].

The action of native staphylotoxin has been tested on T lymphocytes obtained from 21 healthy human donors. As revealed in this investigation, toxic action affects Fc-receptors to IgG, IgM and IgA. The dose-dependent effect of the preparation suggests its direct contacts with Fc-receptors of lymphocytic membranes.

[The effect of the physical hygienic properties of the materials of protective clothing on the length of time working wearing it].

Experimental correlation of physical and chemical properties of materials and its protective properties was found. This correlation may be used in screening of unprotective materials.

[Work capacity of man when using individual respiratory protective devices].

Gas-mask decreased the ability to work by 20-50%. It was caused by decreasing skin and respiratory evaporation. Ventilation of the under mask interspace (90 1/min) was recommended.

[Effects of taktivin on antibody-forming splenic cells in rats].

The paper studied the effect of Taktivin on antibody forming cells of the spleen of Wistar rats which were injected in doses 0.5 Hg and 1.0 Hg.

[Leukocytic index of intoxication and immunologic disorders in diffuse suppurative peritonitis].

Expression of total T- and B-population, T-subpopulation lymphocyte receptors and secretion of IgM, IgG and IgA were measured in 112 patients with diffuse purulent peritonitis and 81 healthy donors. Expression of receptors was correlated with the disease severity identified by leukocytic index of intoxication (LII). The latter appeared to accurately reflect the pattern of immunological impairment.

[Protective effect of anti-staphylococcal immunosorbent on the phenotype of lymphocyte membrane in diffuse Staphylococcus-Pseudomonas peritonitis].

An antistaphylococcal and an antitetanic immunosorbents on a silica carrier were tested in diffuse staphylococcal pyocyanic peritonitis to protect receptor expression on the general T and B lymphocyte populations and of Fc-receptors to IgM, IgG and IgA. Plasma of animals with peritonitis was shown to exert a powerful suppressive effect on lymphocyte receptors. Perfusion of toxic plasma over the antistaphylococcal immunosorbents resulted in specific adsorption of microbial toxins and completely prevented Fc gamma-, Fc mu- and Fc alpha-receptor expression on cells.

[Reaction of peripheral blood leukocytes to wound processes during phytohemagglutinin therapy in rabbits with different hypersensitivity of delayed type].

Altogether 165 rabbits were distributed into strongly and poorly reacting groups in terms of delayed type hypersensitivity (DTH) to phytohemagglutinin (PHA). It was established that leukocyte response to wound process correlated with the intensity of DTH. The strongly reacting animals manifested of DTH.