Chemosensory signalling in human sperm is controlled by Ca influx via CatSper and Ca clearance via plasma membrane Ca ATPases.

Background And Purpose: Loss of function of the sperm-specific Ca channel CatSper is a common channelopathy that causes male infertility. CatSper controls the intracellular Ca concentration and, thereby, the motility of human sperm. Activation of CatSper by oviductal ligands evokes a transient Ca increase, which entails changes in the flagellar beat that are required for fertilisation.

Prospects of digital scientific publishing on blockchain: The concept of DAP.

Background: Traditional publishing models, open access and major publishers, cannot adequately address the key challenges of academic publishing today: Speed of peer review, recognition of work and incentive mechanisms, transparency and thrust of the system.

Methods: To address these challenges, the authors propose Democratisation of Academic Publishing (DAP) platform, which is based on the novel HashNET DLT platform. The DAP introduces several innovative components: tracking the activities of all participants in the peer review process using blockchain and smart contracts, the introduction of the Scholarly Wallet for holding reputation (non-fungible) and reward (fungible) tokens, the use of the Scholarly Wallet as the main interface to the DAP platform, the Virtual Editor that enables automatic discovery of the research area and invitation of reviewers, and finally the global database of evaluated reviewers, ranked by the quality of their previous work.

Exploring potential therapeutic combinations for castration-sensitive prostate cancer using supercomputers: a proof of concept study.

To address the challenge of finding new combination therapies against castration-sensitive prostate cancer, we introduce Vini, a computational tool that predicts the efficacy of drug combinations at the intracellular level by integrating data from the KEGG, DrugBank, Pubchem, Protein Data Bank, Uniprot, NCI-60 and COSMIC databases. Vini is a computational tool that predicts the efficacy of drugs and their combinations at the intracellular level. It addresses the problem comprehensively by considering all known target genes, proteins and small molecules and their mutual interactions involved in the onset and development of cancer.

Psychedelic Drug Microdosing Practices: A Qualitative Online Exploration.

"Microdosing," defined as the consumption of small, sub-hallucinogenic quantities of psychedelic drugs, has gained recent popularity. Microdosing is a relatively new concept, therefore no scientific recommendations exist on how to prepare and consume microdoses. Many consumers obtain microdosing information online.

EPLIN-α and -β Isoforms Modulate Endothelial Cell Dynamics through a Spatiotemporally Differentiated Interaction with Actin.

Actin-binding proteins are essential for linear and branched actin filament dynamics that control shape change, cell migration, and cell junction remodeling in vascular endothelium (endothelial cells [ECs]). The epithelial protein lost in neoplasm (EPLIN) is an actin-binding protein, expressed as EPLIN-α and EPLIN-β by alternative promoters; however, the isoform-specific functions are not yet understood. Aortic compared to cava vein ECs and shear stress-exposed cultured ECs express increased EPLIN-β levels that stabilize stress fibers.

Identification of atheroprone shear stress responsive regulatory elements in endothelial cells.

Aims: Oscillatory shear stress (OSS) is an atheroprone haemodynamic force that occurs in areas of vessel irregularities and is implicated in the pathogenesis of atherosclerosis. Changes in signalling and transcriptional programme in response to OSS have been vigorously studied; however, the underlying changes in the chromatin landscape controlling transcription remain to be elucidated. Here, we investigated the changes in the regulatory element (RE) landscape of endothelial cells under atheroprone OSS conditions in an in vitro model.

Occurrence of protein disulfide bonds in different domains of life: a comparison of proteins from the Protein Data Bank.

Disulfide bonds (SS bonds) are important post-translational modifications of proteins. They stabilize a three-dimensional (3D) structure (structural SS bonds) and also have the catalytic or regulatory functions (redox-active SS bonds). Although SS bonds are present in all groups of organisms, no comparative analyses of their frequency in proteins from different domains of life have been made to date.

Improving the selectivity of antimicrobial peptides from anuran skin.

Anuran skin is known to be a rich source of antimicrobial peptides although their therapeutic potential is often limited due to their toxicity against mammalian cells. The analysis of structure-activity relationships among anuran antimicrobial peptides provided the parameters to construct the "Mutator" tool for improving their selectivity for bacterial cells, by suggesting appropriate point substitutions. Double substitution analogues [K2, K16] of the Xenopus tropicalis peptide XT-7 and [I2, K19] of the Ascaphus truei peptide ascaphin-8 were predicted by this tool to have an increased 'therapeutic index' (TI = HC(50)/MIC for erythrocytes with respect to bacteria) > 80.

DADP: the database of anuran defense peptides.

Anuran tissues, and especially skin, are a rich source of bioactive peptides and their precursors. We here present a manually curated database of antimicrobial and other defense peptides with a total of 2571 entries, most of them in the precursor form with demarcated signal peptide (SP), acidic proregion(s) and bioactive moiety(s) corresponding to 1923 non-identical bioactive sequences. Search functions on the corresponding web server facilitate the extraction of six distinct SP classes.

Knowledge-based computational methods for identifying or designing novel, non-homologous antimicrobial peptides.

We describe computational approaches for identifying promising lead candidates for the development of peptide antibiotics, in the context of quantitative structure-activity relationships (QSAR) studies for this type of molecule. A first approach deals with predicting the selectivity properties of generated antimicrobial peptide sequences in terms of measured therapeutic indices (TI) for known antimicrobial peptides (AMPs). Based on a training set of anuran AMPs, the concept of sequence moments was used to construct algorithms that could predict TIs for a second test set of natural AMPs and could also predict the effect of point mutations on TI values.

[Surgical treatment of Arnold-Chiari malformation type I in an adult patient].

Background: Herniation of the cerebellar tonsils through the foramen magnum into the cervical spinal canal with obliteration of the cerebellomedullary cistern is the primary feature of Arnold-Chiari type I malformation (ACM I). It is considered to be congenital malformation, although there have been reported cases of an acquired form.

Case Report: We presented a female patient, 45-year old, with ACM I without syringomyelia as a rare and unusual clinical image, as well as the effect of decompressive surgery in the treatment of this malformation.

Hormone receptors in benign intracranial meningiomas.

Purpose: Although some embryologic data suggest that the neural crest elements, which are not a target tissue for estrogen and progesterone, participate in the forming of meningothelial cells, meningiomas show hormone receptors. The aim of this study was to investigate the estrogen (ER) and progesterone receptors (PR) content of benign meningiomas (WHO grade I) which were completely resected.

Materials And Methods: Paraffin-embedded tissue sections of 30 intracranial meningiomas were examined immunohistochemically using monoclonal antibodies for ER and PR.

[Significance of sensory evoked potentials in determination of the level of brachial plexus injuries].

The aim of this work is to show the highlights of electrophysiological diagnostics, i.e. its potentials in level diagnostics of traumatic disfunction of brachial plexus (BP).