ANALYSIS OF THE INFLUENCE OF PROLONGED IRRADIATION ON HEMATOPOIETIC PROGENITOR CELLS IN GEL DIFFUSION CHAMBERS USING MATHEMATICAL MODELLING.

Objective: determining of the functional activity of mice bone marrow hematopoietic progenitor cells, cultivated in gel diffusion chambers, on the stages of hematopoiesis recovery after their prolonged irradiation in the lethal dose in a comparative aspect with the method of colony forming in spleen using mathematical model.

Materials And Methods: The method of cell cultivation in gel diffusion chambers, cytological methods, mathematical modeling, and statistical methods of research were used. Bone marrow samples extracted from the femur of mice irradiated with a total dose of 8 Gy with a power 0.

HUMANIZED MODEL OF ISOLATED SUSPENSION CULTIVATION OF HEMATOPOIETIC PROGENITOR CELLS FOR THE INVESTIGATION OF IONIZING RADIATION INFLUENCE IN VIVO.

Objective: development of the humanized system for cells cultivation outside the human organism (human-mouse)and investigation of the influence of ionizing radiation in increasing doses on the colony-forming ability ofhematopoietic progenitor cells.

Materials And Methods: Bone marrow samples of individuals without blood system diseases were cultivated in geldiffusion chambers with semi-solid agar in the abdominal cavity of CBA mice exposed to ionizing radiation action.Cell aggregates, which were obtained in the culture of diffusion chambers in vivo, were counted and colony-formingefficiency of bone marrow cells was determined.

COMPARATIVE MATHEMATICAL ANALYSIS OF COLONY-FORMING ABILITYOF THE BONE MARROW OF MICE LETHALLY AND NON-LETHALLY IRRADIATED WITH EQUAL DOSE RATE INTENSITY.

Objective: To perform comparative analysis of the characteristics of population functioning process of mice bonemarrow colony-forming units after their prolonged irradiation in lethal and non-lethal doses with equal dose rateintensity with the aid of mathematical model.

Materials And Methods: Assigned task is solved by means of mathematical model of alterations in the number ofbone marrow colony-forming units after continuous irradiation, described in previous works, with the use of experimental results of K. S.

MATHEMATICAL ANALYSIS OF FUNCTIONAL PROPERTIES OF MICE BONE MARROW IN THE INITIAL PHASE OF ACUTE FRACTIONATED IRRADIATION.

Objective: to determine the quantitative characteristics of population functioning of mice bone marrow colony-forming units during seven days of acute fractionated irradiation.

Materials And Methods: Assigned task is solved by means of described in works R. V.

Development of -containing scintillating bolometers for a high-sensitivity neutrinoless double-beta decay search.

This paper reports on the development of a technology involving -enriched scintillating bolometers, compatible with the goals of CUPID, a proposed next-generation bolometric experiment to search for neutrinoless double-beta decay. Large mass ( ), high optical quality, radiopure -containing zinc and lithium molybdate crystals have been produced and used to develop high performance single detector modules based on 0.2-0.

[Specific aspects of pathogenesis and strategies in the comprehensive surgical treatment of the diabetic foot in patients with diabetes mellitus].

Retrospective analysis of complex surgical treatment of 590 patients with diabetes mellitus (DM), complicated by necrotic-inflammatory affection (NIAF) of foot for 1991-2000 yrs period was conducted. At the time of first hospitalization age of patients had constituted at average (60.3 +/- 0.

[Method of counterstaining preparations for immunofluorescent studies of the pituitary].

To elucidate nonfluorescent structural elements of the hypophyseal parenchyma for immunofluorescent investigations, properties of some dyes most commonly applied for hypophysis staining have been studied. Such dyes as paraldehide-fuchsin, light green, orange G, chromotrop 2R, hematoxylin, eosin, fuchsin, azocarmin possess their own intensive luminescence and block immunofluorescence completely. Some other dyes (trypan blue, bromthymol blue, aniline blue, malachite green, methyl green) though not blocking immunofluorescence, they do not reveal hypophyseal cellular elements distinctly enough.

[Modification of the Heidenhain's method of staining with iron hematoxylin for detection of the A-cells of the islands of Langerhans].

The authors suggest to use iron hematoxylin according to Haidenhain's prescription, with the subsequent passage of stained and differentiated histological sections through the ammonia solution for detection of A-cells in the pancreatic islets. The A-cells cytoplasm stained dark blue intensively; as to the nuclear structures--they acquired bluish-black tints. The method can be used for the study of morphology, topography and the number of A-cells in the islets.

[Immunohistochemical characteristics of cells of the adenohypophysis producing adrenocorticotropic hormone].

By means of immune fluorescence and histochemical methods, adenohypophyseal corticotropocytes from intact rats and the rats subjected to immobilization stress were studied. The data obtained demonstrated that according to their tinctorial properties, corticotropocytes are chromophobic adenocytes and some of them contain small aldehyde-fuchsinophil and PAS-positives granules. After an acute stress, corticotropocytes secrete ACTH into the intracellular space, and the cytoplasmic processes secrete it into the pericapillar space.